[1]郭晓姝,方海立,李军,等.颈交感神经阻滞对烧伤早期小鼠肝脏干扰素诱导蛋白IFIT1表达的影响[J].第三军医大学学报,2007,29(17):1647-1649.
 GUO Xiao-shu,FANG Hai-li,LI Jun,et al.Effect of cervical sympathetic block on hepatic interferon-induced protein IFIT1 in burned mice during early stage[J].J Third Mil Med Univ,2007,29(17):1647-1649.
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颈交感神经阻滞对烧伤早期小鼠肝脏干扰素诱导蛋白IFIT1表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第17期
页码:
1647-1649
栏目:
论著
出版日期:
2007-09-15

文章信息/Info

Title:
Effect of cervical sympathetic block on hepatic interferon-induced protein IFIT1 in burned mice during early stage
作者:
郭晓姝方海立李军粟永萍
第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室;重庆医科大学基础医学院生理学教研室
Author(s):
GUO Xiao-shu FANG Hai-li LI Jun SU Yong-ping
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury,  College of Preventive Medicine,  Third Military Medical University; Chongqing 400038; Department of Physiology, School of Preclinical Medicine, Chongqing Medical University,Chongqing 400016,China
关键词:
IFIT1颈交感神经阻滞烧伤
Keywords:
IFIT1 cervical sympathetic block burn injury
分类号:
R392.11;R614.4;R644.02
文献标志码:
A
摘要:
目的  检测干扰素诱导蛋白 IFIT1(interferoninduced protein with tetratricopetide repeats 1)在小鼠各器官组织的表达状况,并在此基础上探讨颈交感神经阻滞(SB)对烧伤后早期小鼠肝脏IFIT1表达的影响。  方法  雄性C57/129小鼠50只,分为对照组和SB治疗组,TBSA 15%~20% Ⅲ度小鼠烧伤模型,分别于烧伤前、烧伤后1、6、12 h和24 h取肝组织,采用免疫印迹法(Western blot)观察IFIT1的蛋白表达情况,同时提取正常小鼠心、肝、脾、肺、肾、小肠、淋巴细胞和骨骼肌组织RNA,以RT-PCR检测IFIT1表达情况。  结果  RT-PCR结果显示IFIT1在正常小鼠各组织均有表达,但组织间的表达量存在差异。Western blot结果表明对照组烧伤后肝组织IFIT1表达显著增高(P<0.01),烧伤后SB治疗组IFIT1表达量较对照组相应时相点降低非常显著(P<0.01)。  结论  IFIT1在小鼠体内广泛表达。SB对严重创伤的治疗作用可能是通过抑制肝内IFIT1表达来实现的。
Abstract:
Objective    To study the expression of interferon-induced protein with tetratricopetide repeats 1 (IFIT1)in various organs of mice and detect the effect of cervical sympathetic block (SB) on hepatic IFIT1 during the early stage in burned mice.       Methods    Fifty male C57/129 mice inflicted with 15%-20% TBSA full thickness burn injury were divided randomly into control group (untreated by SB) and SB group(treated immediately by SB). Hepatic tissue was taken before burn injury and at 1st h, 6th h, 12th h, and 24th h after burn injury. The protein expression level of IFIT1 was measured by Western blot.  RNA was extracted from the heart, liver, spleen, lung, kidney, small intestine, lymphocyte and skeletal muscle of normal mice; then the RNA expression level of IFIT1 was detected by RT-PCR.       Results    The RNA expression of IFIT1 was detected in heart, liver, spleen, lung, kidney, small intestine, lymphocyte and skeletal muscle of normal mice, but there was difference in various organs. The result of Western blot displayed that the protein expression level of IFIT1 in control group increased significantly (P<0.01). The level of IFIT1 in SB group was decreased significantly at different time points after burn injury as compared with the control group (P<0.01).        Conclusion    IFIT1 is generally expressed in various organs of mice. SB could enhance the function of GR by exerting suppression on IFIT1 level.

参考文献/References:

郭晓姝,方海立,李军,等.  颈交感神经阻滞对烧伤早期小鼠肝脏干扰素诱导蛋白IFIT1表达的影响[J].第三军医大学学报,2007,29(17):1647-1649.

相似文献/References:

[1]郭晓姝,李洪涛,方海立,等.小鼠IFIT1多克隆抗体的制备[J].第三军医大学学报,2007,29(15):1462.
 GUO Xiao-shu,LI Hong-tao,FANG Hai-li,et al.Preparation of polyclonal antibody of mouse IFIT1[J].J Third Mil Med Univ,2007,29(17):1462.
[2]李军,粟永萍,王军平,等.颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J].第三军医大学学报,2007,29(08):655.
 LI Jun,SU Yong-ping,WANG Jun-ping,et al.Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice[J].J Third Mil Med Univ,2007,29(17):655.
[3]王军平,粟永萍.交感神经调节在严重创伤救治中的作用与展望[J].第三军医大学学报,2009,31(01):10.
[4]田君,刘明华,粟永萍,等.颈交感神经阻滞在严重创伤患者早期应用中的初步探讨[J].第三军医大学学报,2010,32(08):830.
 Tian Jun,Liu Minghua,Su Yongping,et al.Early application of cervical sympathetic ganglia block in severe trauma patients: analysis of 30 cases[J].J Third Mil Med Univ,2010,32(17):830.

更新日期/Last Update: 2008-07-21