[1]童林艳,胡长林,李晋芳,等.两种不同开放血脑屏障方法的比较[J].第三军医大学学报,2007,29(18):1749-1751.
 TONG Lin-yan,HU Chang-lin,LI Jin-fang,et al.Permeability of blood-brain barrier by ultrasound-induced microbubble destruction or mannitol osmotic opening in rats[J].J Third Mil Med Univ,2007,29(18):1749-1751.
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两种不同开放血脑屏障方法的比较(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1749-1751
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Permeability of blood-brain barrier by ultrasound-induced microbubble destruction or mannitol osmotic opening in rats
作者:
童林艳胡长林李晋芳王志刚程远
重庆医科大学附属第二医院:神经内科,超声影像学研究所,神经外科
Author(s):
TONG Lin-yan HU Chang-lin LI Jin-fang WANG Zhi-gang CHENG Yuan
Department of Neurology, Ultrasonography Institute,Department of Neurosurgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
关键词:
超声波微泡造影剂血脑屏障伊文思蓝
Keywords:
ultrasoundmicrobubbleblood brain barrierevans blue
分类号:
R331.54;R445.1
文献标志码:
A
摘要:
目的  比较超声波诱导微泡破坏和甘露醇渗透性开放两种方法对大鼠血脑屏障通透性的影响。 方法  大鼠分为2个实验组和1个对照组,1个实验组大鼠经股静脉注入微泡造影剂后,采用频率43 kHz、声强1.2 W/cm2的超声波经大鼠颅骨照射3 min;另1个实验组大鼠经颈内动脉注入20%甘露醇。干湿重法测定脑水含量,荧光显微镜观察伊文思蓝的渗出。 结果  两种方法都可以可逆开放血脑屏障,利用超声波诱导微泡破坏法开放血脑屏障较甘露醇渗透性开放法局限且持久。 结论  超声波诱导微泡造影剂破坏是一种无创和具靶向性开放血脑屏障的新方法。
Abstract:
Objective    To compare the permeability of blood-brain barrier (BBB) by 2 different opening methods, ultrasound-induced microbubble destruction and mannitol osmotic opening in rats.     Methods    Totally 80 Wistar rats were divided into ultrasound group (A, n=35), mannitol group (B, n=35) and saline group (C, n=10). The rats of group A were sonicated by ultrasound(43 kHz, 1.2 W/cm2)for 3 min after microbubbles injected through femoral vein, and those from group B received an injection of 20% mannitol into the internal carotid artery, those of group C got saline injection into femoral vern. Brain water content was determined by dry-wet weight method. Evans blue dye exudation in the brain was observed with fluorescence microscopy.     Results    Two methods opened BBB reversibly, and the effects of ultrasound-induced microbubble destruction in opening BBB were more localized and durable than mannitol osmotic opening.     Conclusion    Ultrasound-induced microbubble destruction is a promising method for noninvasively and targeted opening BBB.

参考文献/References:

童林艳,胡长林,李晋芳,等.  两种不同开放血脑屏障方法的比较[J].第三军医大学学报,2007,29(18):1749-1751.

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更新日期/Last Update: 2008-07-16