[1]周永,糜漫天,朱俊东,等.三羟异黄酮对小鼠辐射损伤后造血功能的影响[J].第三军医大学学报,2007,29(10):866-868.
 ZHOU Yong,MI Man-tian,ZHU Jun-dong,et al.Effect of genistein on hematopoiesis in irradiated mice[J].J Third Mil Med Univ,2007,29(10):866-868.
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三羟异黄酮对小鼠辐射损伤后造血功能的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
866-868
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Effect of genistein on hematopoiesis in irradiated mice
作者:
周永糜漫天朱俊东郎海滨
第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室
Author(s):
ZHOU Yong MI Man-tian ZHU Jun-dong LANG Hai-bin
Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing 400038, China
关键词:
三羟异黄酮辐射损伤血清
Keywords:
genistein radiation injury serum
分类号:
R551;R818.74;R977.12
文献标志码:
A
摘要:
目的    探讨三羟异黄酮(genistein, GEN)对辐射损伤造血功能的影响。    方法    6.0 Gy γ射线一次全身照射BALB/c雄性小鼠复制出造血损伤模型。照射前24 h给予GEN灌胃(160 mg/kg体质量),分别观察照射后1、3、7、14、21、30 d不同时相点小鼠血清对粒单系集落形成单位(colony-forming unit-granulocyte/macrophage, CFU-GM)和红系集落形成单位(colony forming unit-erythroid, CFU-E)的支持或抑制活性。    结果    辐射前24 h给予GEN,辐射后该组小鼠血清造血刺激活性显著增加,使正常小鼠骨髓有核细胞CFU-GM、CFU-E形成数量明显增多,而且对CFU-GM的刺激活性较CFU-E强。同时,该组小鼠照后血清对CFU-GM、CFU-E的抑制活性明显降低,但对CFU-E和CFU-GM的抑制活性差异不明显。    结论    提高辐射损伤后小鼠血清刺激活性可能是GEN防护放射性造血损伤、促进受损造血系统重建的分子机制之一。
Abstract:
Objective    To study the effect of genistein on the hematopoiesis in irradiated mice.     Methods    Adult male BALB/c mice were administered orally with genistein (160 mg/kg) 24 h before whole body exposure to gamma irradiation at a sublethal dose of 6.0 Gy. On day 1, 3, 7, 14, 21 and 30 after irradiation injury, the hematopoiesis-stimulating and hematopoiesis-suppressing activities of the serum were determined by CFU-E and CFU-GM culture in vitro, respectively.     Results    The hematopoiesis-stimulating activities of serum enhanced distinctly in all irradiated groups. With genistein administration 24 h before irradiation, the hematopoiesis-stimulating activities of serum became enhanced, resulting in the increase of numbers of CFU-GM and CFU-E, as compared to those in only irradiated mice, and stimulating activities on CFU-GM were more enhanced than that on CFU-E. The hematopoiesis-suppressing activities of serum were decreased in genistein pre-treated mice, but showed no significant difference between CFU-GM and CFU-E.     Conclusion    Enhancing hematopoiesis-stimulating activity of serum in the irradiated mice may be one of the important mechanisms of genistein against irradiation-induced hematopoietic syndrome and accelerating restoration of hematopoiesis.

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更新日期/Last Update: 2008-10-24