[1]杨继飞,张建,景秀京,等.糖原合酶激酶3β在大鼠脑老化及神经退行性变中的表达与分布改变[J].第三军医大学学报,2007,29(19):1873-1876.
 YANG Ji-fei,ZHANG Jian,JING Xiu-jing,et al.Distribution and expression changes of glycogen synthase kinase-3β in aged and Aβ-induced neurodegenerative rat brain[J].J Third Mil Med Univ,2007,29(19):1873-1876.
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糖原合酶激酶3β在大鼠脑老化及神经退行性变中的表达与分布改变(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第19期
页码:
1873-1876
栏目:
论著
出版日期:
2007-10-15

文章信息/Info

Title:
Distribution and expression changes of glycogen synthase kinase-3β in aged and Aβ-induced neurodegenerative rat brain
作者:
杨继飞张建景秀京胡远兵杨忠
武警四川总队成都医院内一科; 第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所
Author(s):
YANG Ji-fei ZHANG Jian JING Xiu-jing HU Yuan-bing YANG Zhong
Department of Neurology, Chengdu General Hospital of Armed Police Forces, Chengdu 610041; Department of Neurobiology, Third Military Medical University, Chongqing 400038, China
关键词:
糖原合酶激酶3β神经退行性变大鼠
Keywords:
glycogen synthase kinase 3β neurodegeneration rats
分类号:
R345.55; R741.02; R749.16
文献标志码:
A
摘要:
目的  比较糖原合酶激酶3β(glycogen synthase kinase-3β, GSK3β)在正常成年、老年及β淀粉样蛋白(amyloid beta, Aβ)诱导的神经退行性变模型大鼠脑内的表达与分布差异,探讨其在神经退行性变中的功能意义。 方法  立体定位仪下海马内注射Aβ1-42建立神经退行性变模型,免疫组织化学观察GSK3β在不同大鼠脑内的分布与定位,Western印迹法定量检测GSK3β在皮层、海马等区域的表达。 结果  GSK3β在脑内分布广泛,主要定位于神经元样细胞,在皮层锥体细胞层、海马锥体细胞层、齿回颗粒层、纹状体及丘脑等区域分布较为密集。老年及Aβ注射组(一侧)GSK3β免疫阳性细胞密度明显增多,以Aβ注射组为甚,阳性信号除细胞体外还延伸至长突起远端。蛋白定量检测显示GSK3β在老年和Aβ注射大鼠较正常成年大鼠呈现明显增高。 结论  在脑老化及Aβ诱导的神经退行性变中存在GSK3β的表达上调,它可能在相关神经病理事件中扮演了重要角色。
Abstract:
Objective    To compare the distribution and expression differences of glycogen synthase kinase-3β(GSK3β) among normal adult, aged and amyloid beta(Aβ)-induced neurodegenerative rat brains, so as to explore its functional role in neurodegeneration.     Methods    Aggregated Aβ was microinjected into normal adult rat hippocampus under a stereotaxic system. The rats over 12 months were defined as aged rats. The distribution and localization of GSK3β were examined using immunohistochemistry. Western blotting was performed to assess expression change in cortex and hippocampus quantitatively.     Results    The GSK3β positive cells were distributed extensively around the whole brain and almost with neuron-like morphology. In normal adult rats, the strong anti-GSK3β immunoreactivity located in the neocortex pyramidal layer, hippocampus pyramidal layer, dentate gyrus, thalamus, substantia nigra, etc. The amount of GSK3β positive cells was much more in the aged and Aβ-injected group than in normal ones. The immunoreactive signals usually extend to the distal area of neurite in the Aβ-injected ones. Western blot showed that the expression intensity of GSK3β was stronger in the aged and neurodegenerative rat brain than in the normal adult rat brain.     Conclusion    The expression of GSK3β increases apparently in the neurons of aged and Aβ-injected brain. It may play a role in the neurodegenerative process.

参考文献/References:

杨继飞,张建,景秀京,等.  糖原合酶激酶3β在大鼠脑老化及神经退行性变中的表达与分布改变[J].第三军医大学学报,2007,29(19):1873-1876.

更新日期/Last Update: 2008-07-15