[1]李军,粟永萍,王军平,等.颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J].第三军医大学学报,2007,29(08):655-657.
 LI Jun,SU Yong-ping,WANG Jun-ping,et al.Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice[J].J Third Mil Med Univ,2007,29(08):655-657.
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颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第08期
页码:
655-657
栏目:
论著
出版日期:
2007-04-30

文章信息/Info

Title:
Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice
作者:
李军粟永萍王军平艾国平邹仲敏
第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
LI Jun SU Yong-ping WANG Jun-ping AI Guo-ping ZOU Zhong-min
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
颈交感神经阻滞烧伤核因子-κB类固醇受体辅活化子-3
Keywords:
cervical sympathetic block burn injury NF-κB steroid receptor coactivator-3
分类号:
R-332;R45;R644.02
文献标志码:
A
摘要:
目的     观察颈交感神经阻滞(sympathetic block, SB)对烧伤小鼠早期炎症介质核因子-κB(NF-κB)及其辅活化子的影响,初步探讨SB在严重创伤中治疗作用的分子机制。     方法     将雄性昆明小鼠分为正常对照组、烧伤对照组、LSB组(烧伤后使用利多卡因进行SB治疗)、RSB组(烧伤后使用罗哌卡因进行SB治疗),每组5只。烧伤为TBSA 15%~20%Ⅲ度。烧伤后6 h采用Western blotting测定肝组织NF-κB P65、类固醇受体辅活化子-3(SRC-3)的蛋白表达及核转位。     结果     烧伤对照组肝组织NF-κB P65、SRC-3蛋白表达水平及核转位显著增加;与烧伤对照组相比,LSB组NF-κB P65蛋白表达无显著变化,SRC-3蛋白表达及NF-κB P65、SRC-3核转位显著下调;RSB组NF-κB P65、SRC-3蛋白表达及核转位均显著低于烧伤对照组,且比LSB组下调更明显。     结论     烧伤早期(6 h)NF-κB及其辅活化子SRC-3蛋白表达水平及核转位显著上调,SB在严重创伤中的治疗作用可能是通过抑制NF-κB功能活性,降低炎症反应程度而实现的。
Abstract:
Objective     To observe the effect of cervical sympathetic block (SB) on NF-κB and its coactivator during the early stage in burned mice.      Methods     Kunming male mice were randomly divided into normal control, burn control, LSB group (treated immediately by SB with Lidocaine after burn injury) and RSB group (treated immediately by SB with Ropivacaine after burn injury). Third degree burn injury involving 15%-20% of TBSA was produced. At 6 h after burn, the expression and nuclear translocation of hepatic NF-κB P65 and steroid receptor coactivator-3 (SRC-3) were detected by Western blotting.      Results     After burn injury, the expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 were markedly elevated as compared with normal control group. The level of SRC-3 and nuclear translocation of NF-κB P65 and SRC-3 in LSB group were significantly downregulated compared with that of burn control group, but the NF-κB P65 was no significant difference with that of burn control group. The expression and nuclear translocation of NF-κB P65 and SRC-3 in RSB group were obviously lower than burn control group and LSB group.      Conclusion     The expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 are markedly elevated during the early stage of burn injury. SB can exert supression on the function of NF-κB and reduce the degree of systemic inflammatory response, which may be the mechanisms for therapeutic effect of SB on severe trauma.

参考文献/References:

李军,粟永萍,王军平,等. 颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J]. 第三军医大学学报, 2007, 29(8):655-657.

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更新日期/Last Update: 2008-10-27