[1]曾建华,钟玲,徐波.地塞米松对宫颈癌移植瘤生长及凋亡的影响[J].第三军医大学学报,2007,29(20):1985-1987.
 ZENG Jian-hua,ZHONG Ling,XU Bo.Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice[J].J Third Mil Med Univ,2007,29(20):1985-1987.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第20期
页码:
1985-1987
栏目:
论著
出版日期:
2007-10-30

文章信息/Info

Title:
Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice
作者:
曾建华钟玲徐波
重庆医科大学附属第二医院妇产科;深圳市妇女儿童医院妇产科
Author(s):
ZENG Jian-hua ZHONG Ling XU Bo
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010,Department of Obstetrics and Gynecology,Shenzhen Women and Children’s Hospital, Shenzhen 518028, China
关键词:
宫颈肿瘤HeLa细胞裸鼠地塞米松凋亡
Keywords:
cervical cancer HeLa cell nude mouse Dexamethasone apoptosis
分类号:
R73-352;R737.33;R977.1
文献标志码:
A
摘要:
目的  探讨不同时间使用地塞米松对宫颈癌HeLa细胞移植瘤生长及凋亡的影响。  方法  BALB/c裸鼠皮下接种HeLa细胞,于成瘤后测量肿瘤直径,不同时间(第6天和第16天)开始经腹腔内注射相同剂量地塞米松(15 mg·kg-1·d-1), 连用10 d,对肿瘤进行透射电镜观察及原位细胞凋亡检测(Terminal deoxynucleotidyl Transferase BiotindUTP Nick End Labeling,TUNEL)凋亡小体数目。  结果  不同时间使用地塞米松体内注射均抑制宫颈癌HeLa细胞裸鼠移植瘤生长,诱导肿瘤细胞凋亡;不同时间药物注射肿瘤的体积大小有差异(P<0.01);早期注射抑瘤率高(94.36%),电镜下凋亡小体明显,TUNEL检测凋亡小体数目较多;晚期注射抑瘤率低(53.57%),只有早期凋亡表现,TUNEL检测凋亡小体数目较少,组间比较有显著性差异(P<0.01)。  结论  不同时间地塞米松体内注射均能抑制肿瘤生长,诱导凋亡发生,并存在时间依从性。
Abstract:
Objective    To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice.     Methods    HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg·kg-1·d-1) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL.     Results    Dexamethasone repressed the growth of cervical cancer and induced tumor cell apoptosis, but the tumor volumes were of statistical difference between the two groups of different injection start time (P<0.01). The inhibition rate of tumor in mice receiving Dexamethasone early was 94.36%; Obvious apoptotic bodies were observed under EM and the number of apoptotic bodies was more. The inhibition rate of tumor in mice receiving Dexamethasone late was 53.57%; Early apoptosis was observed and the number of apoptotic bodies was less.      Conclusion    Dexamethasone can repress cervical tumor growth and induce tumor cell apoptosis in a time-dependent manner.

参考文献/References:

曾建华,钟玲,徐波.  地塞米松对宫颈癌移植瘤生长及凋亡的影响[J].第三军医大学学报,2007,29(20):1985-1987.

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更新日期/Last Update: 2008-07-11