[1]姜友昭,张玲,郑江,等.不同葡萄糖浓度对INS-1细胞PPARδ表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(03):247-249.
 JIANG You-zhao,ZHANG Ling,ZHENG Jiang,et al.Effects of different concentrations of glucose on PPARδ expression in INS-1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(03):247-249.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第03期
页码:
247-249
栏目:
论著
出版日期:
2007-02-15

文章信息/Info

Title:
Effects of different concentrations of glucose on PPARδ expression in INS-1 cells
作者:
姜友昭张玲郑江陈兵
第三军医大学西南医院:内分泌科,门诊部,中心实验室
Author(s):
JIANG You-zhao ZHANG Ling ZHENG Jiang CHEN Bing
Department of Endocrinology, Department of Outpatient, Central Lab, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
PPARδINS-1细胞RT-PCRWestern blot
Keywords:
PPARδ INS-1 cells  RT-PCR  Western blotting
分类号:
R329.25;R335.6;R392.6
文献标志码:
A
摘要:
目的     研究不同葡萄糖浓度对大鼠INS-1 细胞PPARδ表达的影响,以初步阐明PPARδ在胰岛细胞糖脂代谢关联中的作用。     方法     分别用含3、11、20 mmol/L葡萄糖的RPMI1640培养基,培养INS-1细胞24 h后,冻融法裂解细胞,检测INS-1细胞内的甘油三酯水平。提取RNA和核蛋白,RT-PCR半定量检测PPARδ mRNA,Western blot 检测PPARδ蛋白表达。     结果     随着葡萄糖浓度的升高INS-1细胞内甘油三酯含量增高,而PPARδ mRNA和蛋白的表达均明显降低。     结论     葡萄糖促进,INS-1细胞内甘油三酯沉积,而PPARδ的表达下降可能是高糖条件下胰岛细胞脂质沉积的重要原因。
Abstract:
Objectives     To observe the effects of glucose at different concentrations on the expression of PPARδ in the rat insulinoma INS-1 cells so as to illustrate the function of PPARδ in the cross-talk of lipid metabolism with glucose metabolism in pancreatic cells.      Methods     After cultured in RPMI1640 media containing 3, 11 and 20 mmol/L glucose respectively for 1 h, the cells were used to measure the content of intracellular triglyceride. The RNA and nuclear protein of INS-1 cells were extracted, and then the expression of PPARδ mRNA was detected by semi-quantity RT-PCR and the PPARδ protein was analyzed by Western blotting.      Results     With the increment of the concentrations of glucose, the content of intracellular triglyceride in the cultured cells was elevated, while the expressions of PPARδ mRNA and protein were both down-regulated.      Conclusion     Glucose promotes triglyceride deposition in INS-1 cells, and the down-regulation of PPARδ presumably contributes to the lipid deposition in INS-1 cells during hyperglycemic states.

相似文献/References:

[1]王丽,陈卡,刘凯,等.S-雌马酚改善高糖培养条件下INS-1细胞胰岛素分泌功能的研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(05):386.
 Wang Li,Chen Ka,Liu Kai,et al.S-equol ameliorates insulin secretion of INS-1 cells cultured in high glucose[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(03):386.
[2]姜友昭,张玲,周艳,等.小鼠PPARδ腺病毒载体的构建及其在INS-1细胞中的表达[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):68.
 JIANG You-zhao,ZHANG Ling,ZHOU Yan,et al.Construction of mouse PPARδ adenovirus vector and its expression in INS-1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(03):68.

更新日期/Last Update: 2008-11-13