[1]刘霞,李平,张恩,等.C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化及意义[J].第三军医大学学报,2007,29(15):1455-1457.
 LIU Xia,LI Ping,ZHANG En,et al.Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury[J].J Third Mil Med Univ,2007,29(15):1455-1457.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第15期
页码:
1455-1457
栏目:
论著
出版日期:
2007-08-15

文章信息/Info

Title:
Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury
作者:
刘霞李平张恩刘苹周元国
第三军医大学大坪医院野战外科研究所分子生物学中心,创伤、烧伤与复合伤国家重点实验室
Author(s):
LIU Xia LI Ping ZHANG En LIU Ping ZHOU Yuan-guo
State Key Laboratory of Trauma, Burns and Combined Injury, Molecular Biology Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
C-skiSmad3成纤维细胞辐射
Keywords:
C-ski Smad3 fibroblast radiation
分类号:
R322.99; R394.2; R818.023
文献标志码:
A
摘要:
目的    研究C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化和意义。     方法    软X线照射诱导大鼠皮肤成纤维细胞辐射性损伤,Western blot和RT-PCR观察C-ski、Smad3蛋白和mRNA水平的变化,EMSA检测Smad3转录活性的变化。    结果    辐射后2 h,C-ski、Smad3蛋白和mRNA水平、Smad3转录活性无显著变化,4 h开始,C-ski表达逐渐下降,Smad3表达和转录活性逐渐升高,在8 h分别达到峰谷和峰顶。    结论    C-ski和Smad3参与大鼠皮肤成纤维细胞辐射性损伤的病理过程,辐射后C-ski下降,Smad3升高,并导致Smad3转录活性增强,细胞增殖受抑,凋亡增加。
Abstract:
Objective    To clarify the changes and significance of C-ski and Smad3 in rat skin fibroblast exposed to radiation.     Methods    The radiation induced injury of rat skin fibroblast was made by exposure to Soft X ray. Protein and mRNA expressions of C-ski and Smad3 were detected by Western blot and RT-PCR. The transcriptional activity of Smad3 was observed by electrophoretic mobility shift assay (EMSA).     Results    At 2 h after radiation, there were no obvious changes in protein and mRNA expression levels of C-ski, Smad3 or the transcriptional activity of Smad3. At 4 h, the expression of C-ski began to gradually decrease, reaching the minimum at 8 h, and Smad3 and Smad3 transcriptional activity began to increase, peaking at 8 h.      Conclusion    C-ski and Smad3 are involved in the pathological process of radiation induced fibroblast injury. Radiation can decrease C-ski and increase Smad3 expression, resulting in elevated Smad3 transcription activity, therefore cell proliferation is repressed and cell apoptosis is increased.

参考文献/References:

刘霞,李平,张恩,等.  C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化及意义[J]. 第三军医大学学报, 2007, 29(15):1455-1457.

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更新日期/Last Update: 2008-09-17