[1]陈锦华,杨辉,尹昌林,等.BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用[J].第三军医大学学报,2007,29(08):699-701.
 CHEN Jin-hua,YANG Hui,YIN Chang-lin,et al.Role of BMP2 in differentiation of neural stem cells from anterior subventricular zone into GABAergic neurons[J].J Third Mil Med Univ,2007,29(08):699-701.
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BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第08期
页码:
699-701
栏目:
论著
出版日期:
2007-04-30

文章信息/Info

Title:
Role of BMP2 in differentiation of neural stem cells from anterior subventricular zone into GABAergic neurons
作者:
陈锦华杨辉尹昌林张治元刘仕勇何家全高方友
第三军医大学新桥医院神经外科
Author(s):
CHEN Jin-hua YANG Hui YIN Chang-lin ZHANG Zhi-yuan LIU Shi-yong HE Jia-quan GAO Fang-you
Department of Neurosurgery, Xinqiao Hospital, Third Millitary Medical University, Chongqing 400037, China
关键词:
神经干细胞SVZaBMP2GABA能神经元GFP
Keywords:
neural stem cells SVZa BMP2 GABAergic neuron GFP
分类号:
R329.21;R338.1;R341
文献标志码:
A
摘要:
目的     研究BMP2在SVZa神经干细胞向γ-氨基丁酸(GABA)能神经元分化中的调控作用。     方法     体外分离培养P0昆明小鼠室管膜下区(SVZa)神经干细胞,纯化传代培养3代后,使用不同浓度BMP2诱导SVZa神经干细胞,采用流式细胞仪检测不同浓度BMP2作用下SVZa神经干细胞分化为GABA能神经元的比例;另外利用活体荧光GFP标记GAD67特异性启动子,动态地研究BMP2在SVZa神经干细胞向GABA能神经元分化中的作用。在此基础之上,采用RT-PCR检测不同浓度BMP2作用下Mash1的表达。     结果     不同浓度BMP2作用组分化为GABA能神经元的比例均高于空白对照组,10 ng/ml浓度的BMP2组比例最高;10 ng/ml浓度BMP2组,GAD67-GFP标记阳性细胞数目明显高于对照组;10 ng/ml浓度BMP2组Mash1表达高于其他组。     结论     BMP2促进SVZa神经干细胞向GABA能神经元的分化;10 ng/ml浓度的BMP2显著促进Mash1的表达。
Abstract:
Objective     To investigate the role of BMP2 in the differentiation of SVZa neural stem cells (NSCs) into GABAergic neurons.      Methods     The neural stem cells derived from anterior subventricular zone (SVZa) of Kunming mouse (postnatal day 0, P0) were separated and cultured in vitro. The rate of GABAergic neurons in the differentiated cells of SVZa NSCs induced by BMP2 (1, 5, 10, 20, 100 ng/ml) were detected by flow cytometry (FCM). The role of BMP2 in the differentiation of SVZa NSCs into GABAergic neurons was studied by the GAD67 promoter labeled with GFP. The expression of Mash1 in the SVZa NSCs induced by BMP2 of different concentration was detected by RT-PCR.      Results     FCM and the GAD67 promoter labeled with GFP confirmed that the rates of GABAergic neurons induced by BMP2 were higher than that of blank group. The expression of Mash1 was enhanced by BMP2 in SVZa NSCs, especially by 10 ng/ml BMP2.      Conclusion     BMP2 promotes the differentiation of SVZa NSCs into GABAergic neurons.

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更新日期/Last Update: 2008-10-28