[1]沈世琴,朱京慈.重型颅脑损伤致急性肺损伤发病机制的实验研究[J].第三军医大学学报,2007,29(14):1422-1424.
 SHEN Shi-qin,ZHU Jing-ci.Mechanism of acute lung injury secondary to isolated severe head injury in rats[J].J Third Mil Med Univ,2007,29(14):1422-1424.
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重型颅脑损伤致急性肺损伤发病机制的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第14期
页码:
1422-1424
栏目:
论著
出版日期:
2007-07-30

文章信息/Info

Title:
Mechanism of acute lung injury secondary to isolated severe head injury in rats
作者:
沈世琴朱京慈
第三军医大学护理系
Author(s):
SHEN Shi-qin ZHU Jing-ci
Department of Nursing, Third Military Medical University, Chongqing 400038, China
关键词:
重型颅脑损伤急性肺损伤机制
Keywords:
severe head injury acute lung injury mechanism
分类号:
R392.3;R563.06;R651.150.2
文献标志码:
A
摘要:
目的    从炎性因子角度探讨重型颅脑损伤单一因素致继发肺损伤的机制。    方法    参照第三军医大学大坪医院野战外科研究所第四研究室建立的气动冲击致重型颅脑损伤模型,将22只雄性SD大鼠按随机数字表法分为正常组(n=4)和损伤组(n=18)。损伤组在伤后1、3、7 d各处死6只动物进行观察。用光镜、电镜观察肺组织形态学改变;放射免疫法监测支气管肺泡灌洗液(BALF)中TNF-α和IL-8水平;酶联免疫吸附实验测定BALF中可溶性细胞间黏附分子-1(sICAM-1)的水平。    结果    光镜下损伤组大鼠各时相均可见肺毛细血管充血、炎性细胞渗出及肺泡间隔增宽等病理改变。3 d时肺组织的病理改变评分显著高于1、7 d(P<0.01)。电镜显示,损伤组在各时相均可见血管内皮细胞肿胀,毛细血管充血、炎性细胞渗出,肺泡上皮细胞损伤等改变,以3 d时最为显著。BALF中TNF-α、IL-8、sICAM-1水平在各时相点均明显高于正常组(P<0.01),3 d时达到峰值(P<0.01);相关分析显示,病理改变评分与TNF-α、IL-8水平正相关(r=0.920,0.867, P<0.01),与sICAM-1水平呈中度正相关(r=0.555, P<0.05)。    结论    重型颅脑损伤大鼠在伤后1~7 d均出现显著的肺损伤,3 d时损伤最显著。肺损伤的发生与重型颅脑损伤后肺局部分泌过多的TNF-α、IL-8、sICAM-1等炎性因子有关。
Abstract:
Objective    To study the mechanism of acute lung injury secondary to isolated severe head injury.     Methods    Twenty-two male SD rats were randomly divided into normal group (NG, n=4), and trauma group (TG, n=18) which were sacrificed respectively on day 1, 3, 7. The pathomorphological changes were observed by light and electron microscope. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) in bronchoalveolar lavage fluid (BALF) were investigated by radioassay (RIA). The level of soluble intercellular adhesion molecule-1(sICAM-1) in BALF was measured by enzyme linked immunosorbent assay (ELISA).     Results    Capillary congestion, inflammatory cell infiltration and thickening of alveolar septum were detected in TG from day 1 to 7 by light microscope. The histological score of lung on day 3 in TG was significantly higher than that on day 1 and 7. The ultramicroscopic structure of lung showed swelling of vascular endothelial cells, infiltration of polymorphonuclear neutrophil and monocyte, injury of pulmonary epithelial cells in TG from day 1 to 7. The levels of TNF-α, IL-8 and sICAM-1 in BALF in TG were significantly higher than that in NG and the peak values were on day 3. The histologic score in TG was positively correlated with the levels of TNF-α, IL-8 and sICAM-1 in BALF.     Conclusion    Isolated severe head injury could induce acute lung injury that reached the most severe degree on day 3. The elevation of such proinflammatory cytokines as TNF-α, IL-8 and sICAM-1 in BALF plays an important role in the development of excessive inflammatory response of lung injury secondary to isolated severe head injury.

参考文献/References:

沈世琴,朱京慈. 重型颅脑损伤致急性肺损伤发病机制的实验研究[J]. 第三军医大学学报, 2007, 29(14):1422-1424.

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更新日期/Last Update: 2008-10-07