[1]吴丽娟,刘国栋,陈伟,等.锌指蛋白A20对人单核细胞LPS应答的影响[J].第三军医大学学报,2007,29(14):1388-1390.
 WU Li-juan,LIU Guo-dong,CHEN Wei,et al.Effects of zinc finger protein A20 on lipopolysaccharide responses in human monocytes[J].J Third Mil Med Univ,2007,29(14):1388-1390.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第14期
页码:
1388-1390
栏目:
论著
出版日期:
2007-07-30

文章信息/Info

Title:
Effects of zinc finger protein A20 on lipopolysaccharide responses in human monocytes
作者:
吴丽娟刘国栋陈伟蒋建新朱佩芳
第三军医大学大坪医院野战外科研究所:检验科,第四研究室, 全军交通医学研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
WU Li-juan LIU Guo-dong CHEN Wei JIANG Jian-xin ZHU Pei-fang
Department of Medical Laboratory, State Key Laboratory of Trauma, Burns and Combined injury, Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
锌指蛋白A20单核细胞内毒素RNA干扰技术NF-κBTNF-α
Keywords:
zinc finger protein A20 monocytes lipopolysaccharide RNA interference technique nuclear factor-κB tumor necrosis factor-α
分类号:
R341;R392.11;R392.32
文献标志码:
A
摘要:
目的    调查锌指蛋白A20表达对人单核细胞LPS应答的影响,探讨A20可能的炎症调控作用与机制及其潜在的临床应用价值。    方法    采用细胞转染技术制备锌指蛋白A20人单核细胞转基因细胞模型和基因沉默细胞模型,以LPS攻击细胞,用适时荧光定量PCR技术测定锌指蛋白A20表达水平,用ELISA技术分析细胞核转录因子NF-κB活性及其依赖性炎性细胞因子TNF-α水平。     结果    锌指蛋白A20过表达组细胞遭受LPS攻击后其NF-κB活性和TNF-α水平明显低于LPS对照组(P<0.01),锌指蛋白A20基因表达抑制组细胞其NF-κB活性和TNF-α水平明显高于LPS对照组(P<0.01)。    结论    锌指蛋白A20对人单核细胞LPS应答具有明显的调节作用,可以明显降低炎症重要核转录因子活性和降低促炎细胞因子的表达。对脓毒症过度炎症反应实施干预具有潜在的临床应用价值。
Abstract:
Objective    To investigate the effect of zinc finger protein A20 expression on lipopolysaccharide (LPS) response in human monocytes so as to discuss its regulation roles and mechanism to inflammation in vivo and its potential value of clinical application.     Methods    Cell transfection technique was respectively used to make zinc finger protein A20 transgenic cell model and gene silence cell model, both of which were then treated with LPS. Real-time reverse PCR was employed to analyze the expression of zinc finger protein A20 and ELISA to determine the activity of nuclear transcription factor NF-κB and the levels of dependent inflammatory cytokines including tumor necrosis factor (TNF) alpha.     Results    After LPS treatment, the NF-κB activity and the TNF-alpha level were significantly lower in the cells with overexpressed zinc finger protein A20 than LPS control cells (P<0.01), but significantly higher in zinc finger protein A20 gene silenced cells (P<0.01).     Conclusion    Zinc finger protein A20 can effectively regulate LPS responses in human monocytes and markedly abate expressions of important nuclear factors and proinflammatory cytokines, proving that zinc finger protein A20 has potential clinical value for over inflammatory reaction of sepsis.

参考文献/References:

吴丽娟,刘国栋,陈伟,等. 锌指蛋白A20对人单核细胞LPS应答的影响[J]. 第三军医大学学报, 2007, 29(14):1388-1390.

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更新日期/Last Update: 2008-10-07