[1]干磊,吴雄飞,吴亿.反义α肌动蛋白基因腺病毒载体对TGF-β1刺激的肾小管上皮细胞转分化的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(13):1301-1304.
　GAN Lei,WU Xiong-fei,WU Yi.Inhibiting tubular epithelial myofibroblast transdifferentiation by transgene of antisense vascular smooth muscle alpha actin in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1301-1304.
点击复制

反义α肌动蛋白基因腺病毒载体对TGF-β1刺激的肾小管上皮细胞转分化的影响(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第13期
页码:
1301-1304
栏目:
论著
出版日期:
2007-07-15

文章信息/Info

Title:
Inhibiting tubular epithelial myofibroblast transdifferentiation by transgene of antisense vascular smooth muscle alpha actin in vitro
作者:
干磊;  吴雄飞;  吴亿
第三军医大学西南医院肾科
Author(s):
GAN Lei;  WU Xiong-fei;  WU Yi
Department of Kidney, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
血管平滑肌α肌动蛋白基因反义腺病毒载体; 转化生长因子β1; 肾小管上皮细胞-肌成纤维细胞转分化
Keywords:
antisense vascular smooth muscle alpha adenovirus vector;  transforming growth factor bete1;  tubular epithelial myofibroblast transdifferentiation
分类号:
R322.61; R329.24; R394.2
文献标志码:
A
摘要:
目的    观察大鼠血管平滑肌α肌动蛋白基因反义腺病毒载体（pAdanti-α-SMA）转染对转化生长因子β1（TGF-β1）所诱导的肾小管上皮细胞-肌成纤维细胞转分化（TEMT）的影响。    方法    pAdanti-α-SMA载体构建并鉴定，转染肾小管上皮细胞（TEC）后免疫荧光染色和Western blot检测α-SMA水平。实验分为5组,A组：正常肾小管上皮细胞（TEC，对照组）；B组：TEC＋2 μl  pAdanti-α-SMA；C组：TEC＋1   μg天然TGF-β1；D组：TEC＋1 μg天然TGF-β1＋2 μl pAdanti-α-SMA；E组：TEC＋1 μg天然TGF-β1＋10 μl pAdanti-α-SMA。    结果    成功构建pAdanti-α-SMA，pAdanti-α-SMA转染TEC后抑制了TGF-β1诱导的TEC 的α-SMA水平并呈剂量依赖性。    结论    pAdanti-α-SMA可有效地抑制肾小管上皮细胞的肌成纤维细胞转分化。
Abstract:
Objective    To evaluate the inhibiting effect of antisense vascular smooth muscle alpha adenovirus vector on tubular epithelial myofibroblast transdifferentiation induced by TGF-β1.      Methods    The pAdanti-α-SMA was constructed and identified. The α-SMA expression in tubular epithelial cells (TEC) was detected by immunofluorescence and Western blot. Five groups were designed as control (A), TEC transfected by 2 μl pAdanti-α-SMA (B), TEC induced by TGF-β1 (C), TEC induced by TGF-β1 and transfected by 2 μl pAdanti-α-SMA (D), TEC induced by TGF-β1 and transfected by 10 μl pAdanti-α-SMA (E).      Results    The pAdanti-α-SMA was successfully constructed. The expression of α-SMA in TEC induced by TGF-β1 was inhibited by pAdanti-α-SMA.       Conclusion    Tubular epithelial myofibroblast transdifferentiation can be inhibited by pAdanti-α-SMA.

相似文献/References:

[1]赵凯,陈姗,龚如军,等.GSK3β促进TGF-β1诱导的肾小管上皮HK-2细胞纤维化[J].陆军军医大学学报(原第三军医大学学报),2012,34(19):1921.
　Zhao Kai,Chen Shan,Gong Rujun,et al.GSK3β plays pro-fibrogenic activity through TGF-beta/Smad signaling pathway in renal tubular epithelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1921.
[2]迟雁青,林海英,张涛,等.肾炎四味片可减轻5/6肾切除大鼠的肾脏纤维化[J].陆军军医大学学报(原第三军医大学学报),2013,35(02):127.
　Chi Yanqing,Lin Haiying,Zhang Tao,et al.Shenyansiwei Tablet alleviates renal fibrosis in 5/6 subtotal nephrectomized rats[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):127.
[3]张庆华,卢根生,李雪梅,等.TGF-β1在复发性尿道狭窄发生中的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):837.
　ZHANG Qing-hua,LU Gen-sheng,LI Xue-mei,et al.Effect of transforming growth factor β1 in recurrent urethral stricture[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):837.
[4]谢江,李廷玉,曾丽玲,等.骨髓间充质干细胞促进大鼠皮肤创面愈合的作用及机制研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(23):2316.
[5]曾柱,龙金华.肝癌细胞对树突状细胞白介素-12表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1305.
　Zeng Zhu,Long Jinhua.Hepatocellular carcinoma cell line Bel7402 suppresses IL-12 secretion of dendritic cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1305.
[6]周宝尚,张璟,陶光利.shRNA干扰β-catenin对TGF-β1诱导的肾小管上皮细胞转分化的作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2166.
　Zhou Baoshang,Zhang Jing,Tao Guangli.Short hairpin RNA of β-catenin attenuates TGF-β1-induced epithelial mesenchymal transition in human proximal tubular epithelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):2166.
[7]毛春谱,李小毅,李伟.参芪降糖颗粒对早期糖尿病肾病患者血清TGF-β1、VEGF的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1475.
[8]赵倩倩,邓春,郭春宝,等.宫内致敏及生后高氧暴露对新生小鼠TGF-β1及α-SMA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):642.
　Zhao Qianqian,Deng Chun,Guo Chunbao,et al.Intra-amniotic endotoxin and postborn hyperoxic exposure upregulate transforming growth factor-β1 and α-smooth muscle actin in newborn mice lung[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):642.
[9]兰天,曾志,陈颖华.栀子苷改善CCl4引起的小鼠肝纤维化损伤的实验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1752.
　Lan Tian,Zeng Zhi,Chen Yinghua.Geniposide attenuates liver fibrosis in CCl4-induced mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):1752.
[10]张荣华,闫国和,汪国建,等.慢病毒介导shRNA沉默鼠肝星状细胞TGFβ1对α1Ⅰ型胶原表达的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(05):456.
　Zhang Ronghua,Yan Guohe,Wang Guojian,et al.Lentiviral vector-mediated shRNA silencing of TGFβ1 on Col1α1 expression in rat hepatic stellate cells[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(13):456.

更新日期/Last Update: 2008-10-15