[1]彭惠,洪苏玲,陶永贤,等.糖尿病仓鼠视网膜p38MAPK和TGFβ2的表达[J].第三军医大学学报,2007,29(08):714-716.
 PENG Hui,HONG Su-ling,TAO Yong-xian,et al.Expression of p38MAPK and TGFβ2 in retina of experimental diabetic hamster[J].J Third Mil Med Univ,2007,29(08):714-716.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第08期
页码:
714-716
栏目:
论著
出版日期:
2007-04-30

文章信息/Info

Title:
Expression of p38MAPK and TGFβ2 in retina of experimental diabetic hamster
作者:
彭惠洪苏玲陶永贤彭彦姜蓉
重庆医科大学:附属第一医院眼科,附属第一医院耳鼻咽喉科,基础医学院组织学与胚胎学教研室
Author(s):
PENG Hui HONG Su-ling TAO Yong-xian PENG Yan JIANG Rong
Department of Ophthalmology, Department of Otorhinolaryngology, The First Affiliated Hospital, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China
关键词:
糖尿病视网膜病变RT-PCRWestern blottingp38MAPK信号途径糖尿病仓鼠
Keywords:
diabetic retinopathy RT-PCR Western blotting p38MAPK signal channel diabetic hamster
分类号:
R394.2;R587.2;R774.102
文献标志码:
A
摘要:
目的     探讨p38MAPK、TGFβ2在糖尿病视网膜病变(diabetic retinopathy, DR)发生发展中的作用及其机制。     方法     用链脲佐菌素(streptozotocin, STZ)诱导仓鼠糖尿病(DM)模型,提取视网膜中总RNA,半定量RT-PCR观察视网膜中p38MAPK、TGFβ2 mRNA表达情况,Western blotting检测DM仓鼠p38MAPK、TGFβ2蛋白情况。     结果     DM仓鼠视网膜中p38MAPK、TGFβ2 mRNA表达呈显著升高,蛋白表达增多。     结论     p38MAPK信号途径参与DR的发病机,制阻断p38MAPK的活性可能有助于阻断DR的发生和发展。
Abstract:
Objective     To explore the role of p38MAPK and TGFβ2 in the development of diabetic retinopathy.      Methods     Fifteen Hamsters were induced into diabetic models by intraperitoneal injection of Streptozotocin (40 mg/kg once a day) for 3 d and 13 Hamsters were successfully established, whose blood glucose was over 13.5 mmol/L. Ten Hamsters as controls were intraperitoneally injected of physical saline of the same volume. At 16th week after induction, the total RNA of retina from all sacrificed Hamsters was collected. The mRNA expressions of p38MAPK,TGFβ2 in retina were detected by semi-quantitative RT-PCR and their protein levels by Western blotting.      Results     The mRNA expressions of p38MAPK, TGFβ2 in retina were of high tendency and their protein levels increased.      Conclusion     p38MAPK signal pathway may involve in the pathogenesis of diabetic retinopathy.

参考文献/References:

彭惠,洪苏玲,陶永贤,等. 糖尿病仓鼠视网膜p38MAPK和TGFβ2的表达 [J]. 第三军医大学学报, 2007, 29(8):714-716.

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更新日期/Last Update: 2008-10-28