[1]王静,刘亮,李金梅,等.青蒿琥酯抗人食管癌作用与调控CDC25A表达的关系[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):428-431.
 WANG Jing,LIU Liang,LI Jin-mei,et al.Inhibitory effect of artesunate on human esophageal carcinoma is associated with CDC25A modulation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(05):428-431.
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青蒿琥酯抗人食管癌作用与调控CDC25A表达的关系(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第05期
页码:
428-431
栏目:
论著
出版日期:
2007-03-15

文章信息/Info

Title:
Inhibitory effect of artesunate on human esophageal carcinoma is associated with CDC25A modulation
作者:
王静刘亮李金梅刘江惠郭建文左连富
河北医科大学第四医院河北省肿瘤研究所流式分析室
Author(s):
WANG Jing LIU Liang LI Jin-mei LIU Jiang-hui GUO Jian-wen ZUO Lian-fu
Department of FCM Analysis, Hebei Provincial Tumor Institute, Fourth Hospital Affiliated to Hebei Medical University, Shijiazhuang 050011, China
关键词:
青蒿琥酯肿瘤细胞周期裸鼠CDC25A
Keywords:
artesunate tumor cell cycle nude mice CDC25A
分类号:
R730.23;R735.1;R979.1
文献标志码:
A
摘要:
目的     观察青蒿琥酯(artesunate, Art)对人食管癌Eca109细胞株及裸鼠移植瘤的抑瘤作用,探讨Art诱导肿瘤细胞周期阻滞是否与CDC25A表达有关。     方法     MTT法测定Art对Eca109细胞及正常人外周血单个核细胞(hPBMC)增殖的影响;流式细胞术(FCM)测定肿瘤细胞的周期变化;观察Art对裸鼠人食管癌移植瘤的抑制情况; 采用RT-PCR及Western blotting方法检测CDC25A mRNA和蛋白表达情况。     结果     Art能显著抑制Eca109细胞的增殖, IC50为 (68.80±0.76)μmol/L,而在刀豆蛋白A(concanavalin A, Con A)刺激下hPBMC的增殖则没有明显抑制作用。低浓度Art可将细胞阻滞于G0/G1期,S期细胞显著减少,当浓度达到100 μmol/L时,Art可将细胞阻滞于G2/M期。Art对裸鼠肿瘤的体积抑瘤率最高可达76.4%,质量抑瘤率可达33.2%。Art可显著抑制Eca109细胞CDC25A mRNA及蛋白表达。     结论     Art可抑制食管癌细胞及裸鼠移植瘤的生长,且无明显毒副作用,通过下调CDC25A的表达而调控细胞周期,是Art发挥抑瘤作用的重要机制。
Abstract:
Objective     To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art.      Methods     MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting.      Results     Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells.      Conclusion     Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating  cell cycle through CDC25A down-regulation.

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更新日期/Last Update: 2008-10-30