[1]吴正国,梁贵友,李正勋,等.参芪扶正注射液对心肌挫伤后家兔心肌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):588-591.
　WU Zheng-guo,LIANG Gui-you,LI Zheng-xun,et al.Effects of Senqi Fuzheng injection on myocardial cell apoptosis, Bcl-2 and Bax expressions in rabbits with myocardial contusion[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):588-591.
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参芪扶正注射液对心肌挫伤后家兔心肌细胞凋亡的影响(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第07期
页码:
588-591
栏目:
论著
出版日期:
2007-04-15

文章信息/Info

Title:
Effects of Senqi Fuzheng injection on myocardial cell apoptosis,  Bcl-2 and Bax expressions in rabbits with myocardial contusion
作者:
吴正国; 梁贵友; 李正勋; 刘日辉; 牛义民; 周继红
遵义医学院第一附属医院胸心外科;湖北省仙桃市第一人民医院胸心外科;湖北省襄樊市中心医院胸心外科;第三军医大学大坪医院野战外科研究所第四研究室，创伤、烧伤与复合伤国家重点实验室
Author(s):
WU Zheng-guo;  LIANG Gui-you;  LI Zheng-xun;  LIU Ri-hui;  NIU Yi-min;  ZHOU Ji-hong
Department of Cardiothoracic Surgery, The First Affiliated Hospital of Zunyi Medical College, Zunyi 563003;Department of Cardiothoracic Surgery, The First People’s Hospital of Xiantao, Xiantao 433100; Department of Cardiothoracic Surgery, Central Hospital of Xiangfan, Xiangfan 441000; State Key Laboratory of Trauma, Burns and Combined Injury, Department  4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
参芪扶正注射液; 心肌挫伤; 细胞凋亡; Bcl-2 蛋白; Bax 蛋白; cTnI
Keywords:
Senqi Fuzheng injection;  myocardial contusion;  apoptosis;  Bcl-2 protein;  Bax protein;  cTnI
分类号:
R285.5; R329.28; R654.2
文献标志码:
A
摘要:
目的     探讨参芪扶正注射液对心肌挫伤后家兔心肌细胞凋亡及其Bcl-2和Bax蛋白表达的影响。     方法     健康家兔50只，简单随机化摸球法分为4组，参考组（Reference，n=20）、对照组(Control,n=10)、参芪扶正注射液治疗组（Sen,n=10）和心肌挫伤组(MC,n=10) 。利用BIM-Ⅱ型生物撞击机制备心肌挫伤模型，应用ELISA法测定心肌肌钙蛋白I（cTnI），应用TUNEL法并结合流式细胞术观察心肌细胞凋亡发生的情况，应用免疫组化SABC法观察Bcl-2和Bax蛋白的表达。对照组除不撞击胸部外，余处理均同MC组。     结果     MC组与Sen组在伤后4 h血清cTnI含量均高于各自伤前及对照组相应时相点（P<0.05）；对照组未见TUNEL阳性细胞,MC组和Sen组心肌细胞凋亡指数(AI)分别为(15.686 0±0.688 7)% 和(5.579 0±0.191 7)%(P<0. 05)，凋亡率分别为(6.932 0±0.638 0) %和（3.021 0±0.254 0）%(P<0. 05)；与对照组比较，MC组的Bcl-2 蛋白和Bax蛋白表达光密度值均明显增高(P<0.05)；而Sen组的Bcl-2 蛋白表达光密度值高于MC组(P<0.05) ,Bax 蛋白表达光密度值低于MC组(P<0.05)。     结论     参芪扶正注射液能有效的减少或抑制钝性心肌挫伤家兔心肌细胞的凋亡，对钝性心肌挫伤具有一定的保护作用，其抑制凋亡可能是通过上调Bcl-2蛋白表达和下调Bax蛋白表达来实现的。
Abstract:
Objective     To study the effects of Senqi Fuzheng injection on the apoptosis of myocardial cells and the expressions of Bcl-2 and Bax in experimental rabbits with myocardial contusion (MC).      Methods     Totally 50 healthy rabbits were randomized into 4 groups: reference group (n=20), control group (n=10), Sen group (n=10) and MC group (n=10). MC model was inflicted with aid of BIM-Ⅱbiomedical impact-machine. The apoptosis of myocardial cells was analyzed by TUNEL. The expressions of Bcl-2 and Bax were detected by using immunohistochemistry with SABC method. The rate of apoptosis in myocardial cells was quantified with flow cytometry. Control group was treated similar to the MC group except the impact.      Results     The levels of serum cTnI were significantly increased 4 h after impact in MC and Sen group (P<0.05). Myocardial cell apoptosis was only detected in MC and Sen group, with the apoptosis index (AI) of (15.686 0±0.688 7)% and (5.579 0±0.191 7)% respectively (P<0.05). The rate of apoptosis was (6.932 0±0.638 0) % in MC group and（3.021 0±0.254 0）% in Sen group (P<0. 05). Bcl-2 expression was up-regulated while Bax expression was down-regulated in Sen group compared to those in MC group (P<0.05).      Conclusion     Senqi Fuzheng injection can lower the AI and protect myocardial cells from MC by increasing Bcl-2 expression and decreasing Bax expression.

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更新日期/Last Update: 2008-10-28