[1]李霞,邓华瑜.格尔德霉素对乳腺癌细胞HSP、突变型p53和CDK4表达的影响[J].第三军医大学学报,2007,29(13):1320-1322.
 LI Xia,DENG Hua-yu.Effects of geldanamycin on expression of HSP, mutant p53 and CDK4 in human breast cancer cells[J].J Third Mil Med Univ,2007,29(13):1320-1322.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第13期
页码:
1320-1322
栏目:
论著
出版日期:
2007-07-15

文章信息/Info

Title:
Effects of geldanamycin on expression of HSP, mutant p53 and CDK4 in human breast cancer cells
作者:
李霞邓华瑜
重庆医科大学基础医学院病理生理学教研室
Author(s):
LI Xia DENG Hua-yu
Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China
关键词:
格尔德霉素乳腺癌HSP增殖应激
Keywords:
geldanamycin breast cancer heat shock protein proliferation stress
分类号:
R737.9; R968; R979.14
文献标志码:
A
摘要:
目的    探讨格尔德霉素(geldanamycin,GA)对乳腺癌MDA-MB- 435s细胞中HSP(HSP90、HSP70、HSP27)和癌蛋白(突变型p53、CDK4)表达的影响。    方法    采用MTT法检测GA对乳腺癌MDA-MB-435s细胞的生长抑制作用,RT-PCR检测GA作用后细胞内HSP90、HSP70、HSP27 mRNA表达变化,用Western blot检测GA作用后细胞内HSP90、HSP70、HSP27、突变型p53和CDK4蛋白质表达变化。    结果    不同浓度GA对MDA-MB-435s细胞有生长抑制作用,呈时效量效依赖关系。400 nmol/L GA处理细胞48 h后,细胞内HSP90、HSP70、HSP27 mRNA表达和蛋白表达均增高,以HSP70增高最明显,而突变型p53和CDK4蛋白表达明显减少。    结论    GA通过下调突变型p53和CDK4表达抑制乳腺癌MDA-MB-435s细胞的增殖,同时GA可诱导细胞内HSP90、HSP70和HSP27表达参与细胞应激保护。
Abstract:
Objective    To investigate the effect of geldanamycin (GA) on the expression of HSP (HSP90, HSP70, HSP27) and oncoprotein (mutant p53, CDK4) in human breast cancer cell line MDA-MB-435s.     Methods    Proliferation of MDA-MB-435s cells was measured with MTT assay. The mRNA expression levels of HSP90, HSP70 and HSP27 were detected by RT-PCR. The protein expression levels of HSP90, HSP70, HSP27, mutant p53 and CDK4 were detected by Western blot.     Results    GA inhibited the proliferation of MDA-MB-435s cells in a time-dose dependent manner. The expression level of mRNA and protein of HSP90, HSP70 and HSP27 were increased, and the increasing of HSP70 was the most significant, while the protein expression level of mutant p53 and CDK4 in MDA-MB-435s cells were reduced obviously after 48-hour treatment with 400 nmol/L GA as compared with that of control cells.     Conclusion    GA inhibits the proliferation of MDA-MB-435s cells by down-regulating the level of mutant p53 and CDK4 protein, and GA involves in the protection of cell stress through increasing the expression of HSP90, HSP70 and HSP27.

参考文献/References:

李霞, 邓华瑜. 格尔德霉素对乳腺癌细胞HSP、突变型p53和CDK4表达的影响[J]. 第三军医大学学报, 2007, 29(13):1320-1322.

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更新日期/Last Update: 2008-10-15