[1]滕小春,刘海峰,房殿春,等.NHE-1反义基因对胃癌细胞的酸化作用及其意义[J].第三军医大学学报,2007,29(03):240-242.
 TENG Xiao-chun,LIU Hai-feng,FANG Dian-chun,et al.Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance[J].J Third Mil Med Univ,2007,29(03):240-242.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第03期
页码:
240-242
栏目:
论著
出版日期:
2007-02-15

文章信息/Info

Title:
Acidification of antisense NHE-1 gene transfection on gastric cancer cell line SGC-7901 and its significance
作者:
滕小春刘海峰房殿春杨仕明王国安陈刚
第三军医大学西南医院全军消化内科中心; 武警总医院消化内科
Author(s):
TENG Xiao-chun LIU Hai-feng FANG Dian-chun YANG Shi-ming WANG Guo-an CHEN Gang
Gastroentero-logy Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, Department of Gastroenterology, General Hospital of the Armed Police Forces, Beijing 100039, China
关键词:
NHE1基因真核表达载体 反义基因治疗胃癌
Keywords:
Na+-H+ exchanger 1 eukaryotic expression vector antisense gene therapy gastric cancer
分类号:
R394-33;R394.2;R735.2
文献标志码:
A
摘要:
目的     探讨Na+-H+交换泵-1(Na+-H+ exchanger1,NHE-1)反义基因转染对人胃癌细胞内pH值(intracellular pH,pHi)的调节作用及其在肿瘤基因治疗中的价值。     方法     构建人NHE-1基因反义真核表达载体,采用脂质体法将其转染至SGC-7901胃癌细胞中, 采用荧光探针检测转染前后细胞内pH值的变化以及对细胞增殖和凋亡的影响。     结果     转染反义基因的细胞pHi明显降低,增殖能力降低,细胞凋亡率增加。     结论     NHE-1基因的表达在肿瘤细胞pHi及增殖和凋亡调节中起重要作用。
Abstract:
Objective     To study the effects of the transefection of antisense human Na+-H+ exchanger 1 (NHE-1) on the pHi of SGC-7901 and its potential role in the gene therapy for cancers.      Methods     Eukaryotic expression vector pcDNA3.1 encoding antisense NHE-1 was constructed with recombinant DNA technique and transfected into SGC-7901 with liposome DOTAP. The pHi changes of the transfected cells and untransfected cells was detected with a fluorescent probe, BCECF. The proliferation and apoptosis of the cells was studied with flow cytometry.      Results     Antisense NHE-1 gene transfection induced the acidification, decreased proliferation, and elevated apoptosis of SGC-7901 cells.      Conclusion     The results suggest that NHE-1 gene plays an important role in the regulation of pHi, reproduction and apoptosis of tumor cells. Inhibiting the over-expression and activity of NHE-1 may be of important clinical value.

参考文献/References:

滕小春, 刘海峰, 房殿春, 等. NHE-1反义基因对胃癌细胞的酸化作用及其意义[J]. 第三军医大学学报,2007,29(3):240-242.

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更新日期/Last Update: 2008-11-13