[1]周希瑗,李琳,江雪丰,等.血管内皮生长因子在糖尿病大鼠脉络膜视网膜的表达[J].第三军医大学学报,2007,29(01):47-50.
 ZHOU Xi-yuan,LI Lin,JIANG Xue-feng,et al.VEGF expression in choroid of streptozotocin-diabetic Wistar rats[J].J Third Mil Med Univ,2007,29(01):47-50.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第01期
页码:
47-50
栏目:
论著
出版日期:
2007-01-15

文章信息/Info

Title:
VEGF expression in choroid of streptozotocin-diabetic Wistar rats
作者:
周希瑗李琳 江雪丰 乔岗
重庆医科大学附属第二医院眼科;武警重庆总队医院眼科
Author(s):
ZHOU Xi-yuan LI Lin JIANG Xue-feng QIAO Gang
Department of Ophthalmology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, Department of Ophthalmology, The Chongqing General Troop of Armed Police, Chongqing 400061, China
关键词:
糖尿病视网膜病变糖尿病脉络膜病变免疫组织化学血管内皮生长因子
Keywords:
diabetic retinopathy diabetic choroidopathy immunohistochemistry vessel endothelial growth factor
分类号:
R392.1; R587.2; R770.2
文献标志码:
A
摘要:
目的     在糖尿病的不同病程中,通过免疫组织化学方法观察血管内皮生长因子(vessel endothelial growth factor, VEGF)在糖尿病大鼠脉络膜中表达的时间、部位,比较正常大鼠及糖尿病大鼠视网膜、脉络膜组织中VEGF的表达情况。     方法     选取健康雄性Wistar大鼠,采用随机数字表法分成糖尿病组和正常对照组,用链脲佐菌素(STZ)大剂量一次性腹腔注射诱导1型糖尿病模型。取不同组、不同时期大鼠视网膜脉络膜,利用免疫组织化学方法,检测VEGF蛋白质的表达情况。     结果      ①糖尿病1个月组(M1)、血糖恢复组(Mh)大鼠视网膜脉络膜VEGF蛋白质的表达与正常对照组(M0)无明显区别。②糖尿病2个月组(M2)脉络膜可见VEGF蛋白质的阳性表达(33.3%),视网膜上VEGF蛋白质的阳性表达与正常对照组无明显区别。③糖尿病3个月组(M3)脉络膜VEGF蛋白表达阳性(55.6%),视网膜的内核层及节细胞层VEGF蛋白表达阳性与正常对照组(33.3%)比较有明显差异。④糖尿病4个月组(M4)脉络膜VEGF蛋白表达阳性(66.7%),视网膜的内界膜、内核层、外核层及节细胞层VEGF蛋白表达阳性(77.8%)。⑤ 糖尿病5个月组(M5)脉络膜VEGF蛋白表达阳性(88.9%),视网膜全层均有VEGF蛋白阳性表达(88.9%)。VEGF在脉络膜和视网膜的阳性染色密度随病程逐渐增加(P<0.05)。     结论     VEGF在大鼠脉络膜视网膜中的表达与病程有关,VEGF蛋白在脉络膜的表达先与视网膜。
Abstract:
Objective     To investigate the expression time and the position of VEGF in the choroid of diabetic rats and compare them with the VEGF expression in the choroid and retina of normal rats.      Methods     Healthy male Wistar rats were selected and randomly divided into diabetic group and normal group. Type 1 diabetes rat model was induced by being injected of large-dose streptozotocinum (STZ) into abdominal cavity. The retina and choroid of rats were obtained to detect the VEGF expression by immunohistochemistry at 1st, 2nd, 3rd, 4th, 5th month after diabetes induction.      Results     VEGF expression in retina and choroid of rats was of obvious difference between normal group and one-month diabetic group. In two-month diabetic group, VEGF expression in choroid was positive (33.3%), while VEGF expression in retina was not significantly different with that of normal group. In three-month diabetic group, VEGF expression in choroid was positive (55.6%), and VEGF expression in inner nuclear layer and ganglion cell layer of retina was positive (33.3%). In four-month diabetic group, VEGF expression in choroid was positive (66.7%), and VEGF expression in inner limiting membrane, inner nuclear layer, outer nuclear layer and ganglion cell layer of retina was positive (77.8%). In five-month diabetic group, VEGF expression in choroid was positive (88.9%), and VEGF expression in the whole retina was positive (88.9%). The expression density of VEGF in choroid and retina gradually increased (P<0.05).      Conclusion     VEGF expression in choroid and retina of rats is concerned with the pathogenesis of diabetes, and VEGF expression in choroid had precedence over that in retina.

参考文献/References:

周希瑗, 李琳, 江雪丰, 等. 血管内皮生长因子在糖尿病大鼠脉络膜视网膜的表达[J]. 第三军医大学学报,2007,29(1):47-50.

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更新日期/Last Update: 2008-11-13