[1]唐军,徐海伟,周光纪,等.ES细胞来源的神经前体细胞移植Aβ损伤大鼠海马后的分化及对学习记忆的改善[J].陆军军医大学学报(原第三军医大学学报),2007,29(02):108-111.
 TANG Jun,XU Hai-wei,ZHOU Guang-ji,et al.Differentiation of neural precursor cells derived from mouse embryonic stem cells after transplantation into Aβ injured rat hippocampus and the improvement in memory[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(02):108-111.
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ES细胞来源的神经前体细胞移植Aβ损伤大鼠海马后的分化及对学习记忆的改善(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第02期
页码:
108-111
栏目:
论著
出版日期:
2007-01-30

文章信息/Info

Title:
Differentiation of neural precursor cells derived from mouse embryonic stem cells after transplantation into Aβ injured rat hippocampus and the improvement in memory
作者:
唐军徐海伟周光纪范晓棠李达兵杨丽
第三军医大学基础医学部:生理学教研室,神经生物学教研室,重庆市神经科学研究所
Author(s):
TANG Jun XU Hai-wei ZHOU Guang-ji FAN Xiao-tang LI Da-bingYANG Li
Department of Physiology, Department of Neurobiology, College of Medicine,  Third Military Medical University, Chongqing 400038, China
关键词:
胚胎干细胞β淀粉样蛋白 细胞移植分化大鼠
Keywords:
embryonic stem cell β-amyloid  protein cell transplantation differentiation rats
分类号:
R329.2;R338.64;R749.160.5
文献标志码:
A
摘要:
目的     观察来源于小鼠胚胎干细胞的神经前体细胞移植Aβ海马损伤大鼠后的存活、分化以及细胞移植对模型大鼠的治疗作用。     方法     采用改良的无血清方法将表达绿色荧光的小鼠胚胎干细胞定向诱导为神经前体细胞, 移植至单侧海马注射Aβ1-40损伤模型大鼠注射处;免疫荧光双染观察移植细胞的存活、分化。     结果     胚胎体在改良的N2选择性培养基生长5 d后,90%以上的小鼠胚胎干细胞分化为nestin阳性神经前体细胞。移植到模型大鼠海马后神经前体细胞存活良好,EGFP荧光显示移植细胞呈集落生长,Cy3荧光显示大部分细胞分化为GFAP阳性细胞,移植区周围可见一定数量NF200阳性细胞,其中部分发出类似神经元的长突起。行为学结果显示移植大鼠记忆能力较对照组明显改善。     结论     胚胎干细胞来源的神经前体细胞移植Aβ1-40损伤模型大鼠海马后能有效存活,大部分移植细胞分化为胶质细胞,小部分分化为神经元,细胞移植能改善模型大鼠学习记忆功能障碍。
Abstract:
Objective     To observe the survival, differentiation and the therapeutic effect of neural precursor cells (NPCs) derived from mouse embryonic stem cells (ESc) after transplanted into Aβ-injured rat hippocampus.      Methods     NPCs were generated from mouse ESc expressing EGFP with modified serum-free methods and then transplanted into the hippocampus of Alzheimer’s disease (AD) rat model developed by Aβ1-40 microinjection. The survival and differentiation of transplanted NPCs were observed with immunofluorescence methods. The learning and memory ability of  AD rats were investigated.      Results     About 90% of mouse ESc were differentiated into nestin-positive NPCs 5 d after the induction with modified N2 serum-free medium. The grafted NPCs in the hippocampus of AD rats showed good survival rate and grew into GFP positive colonies, and mostly differentiated into GFAP-positive glia and some into NF200-positive neurons with long evection as to the neuron. The ability in learning and memory of AD rats was much improved after NPCs transplantation compared with that of control group.      Conclusion     The NPCs derived from ESc are survived and mostly differentiated into the glial cells and a few into neurons after grafted into the hippocampus of AD rats, and show therapeutic effect on AD as improved learning and memory abilities.

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更新日期/Last Update: 2008-11-13