[1]葛斌,张振明,许爱霞,等.水红花子醇提物抑制大鼠组织脂质过氧化反应的体外作用研究[J].第三军医大学学报,2007,29(06):516-518.
 GE Bin,ZHANG Zhen-ming,XU Ai-xia,et al.Effect of alcoholic extract from Fructus Polygoni Orientalis on lipid peroxidation in rat tissues[J].J Third Mil Med Univ,2007,29(06):516-518.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第06期
页码:
516-518
栏目:
论著
出版日期:
2007-03-30

文章信息/Info

Title:
Effect of alcoholic extract from Fructus Polygoni Orientalis on lipid peroxidation in rat tissues
作者:
葛斌张振明许爱霞高湘雷晓燕
甘肃省人民医院:药剂科,小儿科
Author(s):
GE Bin ZHANG Zhen-ming XU Ai-xia GAO Xiang LEI Xiao-yan
Department of Pharmacy, Department of Pediatrics, The People’s Hospital of Gansu Province, Lanzhou 730000
关键词:
水红花子醇提物丙二醛自由基溶血度红细胞中性粒细胞抗氧化活性
Keywords:
alcoholic extract from Fructus Polygoni Orientalis malondialdehyde free radicals hemolysis erythrocyte neutrophil antioxidation
分类号:
R282.71;R285.5
文献标志码:
A
摘要:
目的     研究水红花子醇提物的抗氧化活性。     方法     用·OH生成系统Fe2++抗坏血酸诱导大鼠心、肝、肾组织匀浆脂质过氧化,用TBA比色法测定MDA含量;用NBT还原法测酵母多糖A刺激大鼠中性白细胞产生的O2-;用分光光度法测H2O2诱发的大鼠红细胞溶血度。     结果     6.3、12.5、25、50、100、200 μg/L EFPO能不同程度抑制Fe2++抗坏血酸诱导的大鼠心、肝、肾脂质过氧化产物MDA生成,其抑制心、肝、肾MDA生成的IC50分别为31.8、 32.5、40.2 μg/L,量效关系均呈负相关,r分别为-0.886、-0.874及-0.918(P<0.01);能不同程度抑制酵母多糖A刺激中性粒细胞生成O2-, IC50为31.9 μg/L,量效关系呈负相关,r为-0.873(P<0.01);能不同程度抑制H2O2诱发的红细胞氧化溶血, IC50为56.2 μg/L,量效关系呈负相关,r为-0.888(P<0.01)。     结论     水红花子醇提物通过清除·OH 、O2-及H2O2发挥抗氧化活性。
Abstract:
Objective     To study the activities of the alcoholic extract from Fructus Polygoni Orientalis (EFPO) against antioxidation.      Methods     The peroxidation in homogenate of rat heart, liver and kidney was induced by ·OH generation system Fe2++ascorbic acid. Malondialdehyde (MDA) was determined by TBA colorimetric method. Superoxide (O2-) from zymosan-stimulated neutrophils of rat was measured by NBT reduction method. H2O2-caused hemolysis of erythrocytes was measured by spectrometry.       Results     MDA in homogenate of heart, liver and kidney of rat that induced by ·OH generation system Fe2++ascorbic acid was inhibited by 6.3, 12.5, 25, 50, 100, 200 μg/L EFPO; IC50 of EFPO to MDA in heart, liver and kidney was 31.8, 32.5 and 40.2 μg/L respectively and its dose-effect relation was of negative relativity and the relative coefficient was -0.886, -0.874 and -0.918 (all P<0.01). O2- generation from zymosan-stimulated neutrophils of rat was inhibited by EFPO; IC50 of EFPO to O2- generation was 31.9 μg/L and its dose-effect relation was of negative relativity and the relative coefficient was -0.873 (P<0.01). H2O2-caused hemolysis of erythrocytes was inhibited by EFPO. IC50 of EFPO to erythrocyte hemolysis was 56.2 μg/L and its dose-effect relation was of negative relativity and the relative coefficient was -0.888(P<0.01).       Conclusion     EFPO exerts its antioxidation by scavenging ·OH, O2- and H2O2 in biological system.

参考文献/References:

葛斌,张振明,许爱霞,等. 水红花子醇提物抑制大鼠组织脂质过氧化反应的体外作用研究 [J]. 第三军医大学学报,2007,29(6):516-518.

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更新日期/Last Update: 2008-10-29