[1]吴远,刘吉宇,张琴.香叶木素调控STX17-AS1/miR-383-5p轴对结直肠癌细胞增殖、凋亡和迁移的影响[J].第三军医大学学报,2021,43(17):1642-1649.
 WU Yuan,LIU Jiyu,ZHANG Qin.Effect of diosmetin on proliferation, apoptosis and migration of colorectal cancer cells by regulating STX17-AS1/miR-383-5p axis[J].J Third Mil Med Univ,2021,43(17):1642-1649.
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香叶木素调控STX17-AS1/miR-383-5p轴对结直肠癌细胞增殖、凋亡和迁移的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
43卷
期数:
2021年第17期
页码:
1642-1649
栏目:
基础医学
出版日期:
2021-09-15

文章信息/Info

Title:
Effect of diosmetin on proliferation, apoptosis and migration of colorectal cancer cells by regulating STX17-AS1/miR-383-5p axis
作者:
吴远刘吉宇张琴
遵义市第一人民医院(遵义医科大学第三附属医院):肛肠科,肿瘤科
Author(s):
WU Yuan LIU Jiyu ZHANG Qin

Department of Anorectal Disorders, 2Department of Oncology, Zunyi First People’s Hospital (Third Affiliated Hospital of Zunyi Medical University), Zunyi, Guizhou Province, 563000, China

关键词:
香叶木素结直肠癌STX17-AS1miR-383-5p细胞增殖迁移凋亡
Keywords:
diosmetin colorectal cancer STX17-AS1 miR-383-5p cell proliferation migration apoptosis
分类号:
R73-361; R735.35; R977.7
文献标志码:
A
摘要:

目的探讨香叶木素对结直肠癌细胞增殖、凋亡和迁移的影响,分析其机制是否与调控STX17-AS1/miR-383-5p轴有关。方法通过细胞计数试剂盒(CCK-8)、集落形成实验、划痕愈合实验、流式细胞术、实时定量PCR(RT-qPCR)分析香叶木素(10、20、40 μmol/L)对结直肠癌细胞HCT116的活力、集落形成数、划痕愈合率、凋亡以及STX17-AS1、miR-383-5p表达的影响。RT-qPCR分析2019年本院29例结直肠癌患者癌组织和对应癌旁组织中STX17-AS1、miR-383-5p相对水平。双荧光素酶报告实验分析STX17-AS1和miR-383-5p的靶向关系。分别转染STX17-AS1的小干扰RNA、miR-383-5p模拟物至HCT116细胞,观察抑制STX17-AS1或过表达miR-383-5p对HCT116细胞增殖、迁移和侵袭的影响。结果香叶木素(10、20、40 μmol/L)处理显著降低HCT116细胞活力、集落形成数、划痕愈合率(P<0.05),增加细胞凋亡率(P<0.05),降低STX17-AS1表达水平(P<0.05),增加miR-383-5p表达水平(P<0.05)。结直肠癌组织中STX17-AS1的表达水平较癌旁组织显著升高(P<0.05),miR-383-5p的表达水平较癌旁组织显著降低(P<0.05)。STX17-AS1与miR-383-5p直接结合。抑制STX17-AS1表达显著降低HCT116细胞活力、集落形成数、划痕愈合率(P<0.05),增加细胞凋亡率(P<0.05),增加miR-383-5p表达水平(P<0.05)。过表达miR-383-5p显著降低HCT116细胞活力、集落形成数、划痕愈合率(P<0.05),增加细胞凋亡率(P<0.05)。过表达STX17-AS1或抑制miR-383-5p表达显著减弱香叶木素对HCT116细胞活力、集落形成数、划痕愈合率和凋亡率的影响(P<0.05)。结论香叶木素通过下调STX17-AS1/miR-383-5p轴可抑制结直肠癌细胞增殖和迁移,诱导细胞凋亡。

Abstract:

ObjectiveTo investigate the effect of diosmetin on the proliferation, apoptosis and migration of colorectal cancer cells, and further to analyze whether its mechanism is related to the regulation of STX17-AS1/miR-383-5p axis. MethodsCell counting kit (CCK-8) assay, colony formation test, scratch healing test, flow cytometry and real-time quantitative PCR (RT-qPCR) were used to analyze the effects of diosmetin (10, 20 and 40 μmol/L) on cell viability, colony formation, scratch healing rate, apoptosis, and STX17-AS1 and miR-383-5p expression in HCT116 cells. The relative expression levels of STX17-AS1 and miR-383-5p in cancer tissues and adjacent tissues of 29 patients with colorectal cancer in our hospital were assessed using RT-qPCR. Dual luciferase reporter assay system was employed to analyze the targeting relationship between STX17-AS1 and miR-383-5p. The small interfering RNA of STX17-AS1 and miR-383-5p mimics of STX17-AS1 were transfected into HCT116 cells respectively, and the effects of STX17-AS1 inhibition or miR-383-5p overexpression on the proliferation, apoptosis and migration of HCT116 cells were also analyzed. ResultsDiosmetin treatment (10, 20, 40 μmol/L) significantly reduced cell viability, colony formation numbers and scratch healing rate (P<0.05), increased cell apoptotic rate (P<0.05), decreased STX17-AS1 expression level (P<0.05), and increased the expression level of miR-383-5p in HCT116 cells (P<0.05). The expression level of STX17-AS1 was significantly higher (P<0.05), and that of miR-383-5p was significantly lower in the colorectal cancer tissues than the adjacent tissues (P<0.05). STX17-AS1 directly bound to miR-383-5p. Inhibition of STX17-AS1 significantly reduced cell viability, colony formation numbers and scratch healing rate (P<0.05), increased cell apoptotic rate (P<0.05), and enhanced the miR-383-5p expression level in HCT116 cells (P<0.05). While, miR-383-5p overexpression significantly reduced cell viability, colony formation numbers and scratch healing rate (P<0.05), and increased cell apoptosis rate in the cells (P<0.05). Overexpression of STX17-AS1 or inhibition of miR-383-5p significantly weakened the effect of diosmetin on the above indicators of HCT116 cells (P<0.05). ConclusionDiosmetin inhibits the proliferation and migration of colorectal cancer cells and induces apoptosis by down-regulating the STX17-AS1/miR-383-5p axis.

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更新日期/Last Update: 2021-09-03