[1]伍灵怡,唐黎,张静,等.胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究[J].第三军医大学学报,2020,42(22):2251-2256.
 WU Lingyi,TANG Li,et al.Characteristics of gut microbiota and their correlation with clinical parameters in patients with cholestatic liver disease[J].J Third Mil Med Univ,2020,42(22):2251-2256.
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胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第22期
页码:
2251-2256
栏目:
临床医学
出版日期:
2020-11-30

文章信息/Info

Title:
Characteristics of gut microbiota and their correlation with clinical parameters in patients with cholestatic liver disease
作者:
伍灵怡唐黎 张静雷媛媛胡世陈雨琳 喻晓刚王应邦唐波杨国栋
川北医学院附属医院消化内科1;陆军军医大学(第三军医大学)第二附属医院消化内科2
Author(s):
WU Lingyi1 2 TANG Li2 ZHANG Jing1 LEI Yuanyuan2 HU Shiping2 CHEN Yulin1 YU Xiaogang1 WANG Yingbang1 TANG Bo2 YANG Guodong
Department of Gastroenterology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, 637000; 2Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China
 
关键词:
胆汁淤积性肝病 肠道菌群 16S rRNA
Keywords:
cholestatic liver disease gut microbiota 16S rRNA
分类号:
R322.45;R378;R575
文献标志码:
A
摘要:

目的分析胆汁淤积性肝病的肠道菌群多样性和结构组成及其与临床参数间的相关性。方法收集胆汁淤积性肝病患者(n=16)和健康人群对照者(n=19)的新鲜粪便,采用16S rRNA分析受试者肠道菌群的多样性和结构特征,运用Pearson法分析差异菌属与临床参数间的相关性。结果基线资料显示研究对象的性别、年龄、BMI无统计学差异(P>0.05),血清学指标有统计学差异(P<0.01)。α多样性表明,胆汁淤积性肝病组的Chao1和Shannon指数明显减少,但无统计学差异,β多样性有统计学差异(P<0.01);菌群结构分析发现Bacteroides(拟杆菌属)、Lachnoclostridium、Veillonella(韦荣氏球菌属)、Roseburia(罗斯氏菌属)、Escherichia_shigella(大肠埃希菌志贺氏菌属)在胆汁淤积性肝病组中明显富集(P<0.05),而Blautia(布劳特氏菌属)明显减少(P<0.05)。Pearson法分析表明,Lachnoclostridium与ALP(P<0.01)、GGT(P<0.05)强相关,Veillonella(韦荣氏菌属)与GGT强相关(P<0.05)。结论胆汁淤积性肝病患者与健康人群对照者相比,肠道菌群存在特征性改变,Veillonella(韦荣氏球菌属)相对高丰度表达与胆汁淤积性肝病强相关。

Abstract:

ObjectiveTo investigate the diversity and composition of gut microbiota in patient with cholestatic liver disease, and explore their correlation with clinical parameters. MethodsFresh stool samples from 16 patients with cholestatic liver disease and 19 healthy controls were collected, and 16S rRNA sequencing was used to analyze the diversity of gut microbiota and composition characteristics. Pearson analysis was employed to analyze the correlation between the different bacteria and clinical parameters. ResultsThere were no statistical differences in the baseline data, such as sex, age and body mass index (BMI) in study subjects (P>0.05), but significant differences were observed in serum indexes among them (P<0.01). The results of α diversity showed that Chao1 and Shannon indexes were lower in the study group, but there was no statistical difference (P>0.05). β diversity results indicated that the species diversity had significant difference between 2 group (P<0.01). Further analysis of microbiota composition showed largely relative abundance of Bacteroides, Lachnoclostridium, Veillonella, Roseburia and Escherichia shigella (P<0.05), while the abundance of Blautia were dramatically decreased (P<0.05). Pearson analysis displayed that Lachnoclostridium was strongly correlated with serum level of alkaline phosphatase (ALP) (P<0.01) and gammaglutamyl transferase (GGT) (P<0.05), and Veillonella was also strongly correlated with GGT (P<0.05). ConclusionCompared with healthy controls, the patients with cholestatic liver disease have obvious characteristic changes in the gut microbiota. The relatively high abundance of Veillonella is closely assocatied with cholestatic liver disease.

更新日期/Last Update: 2020-11-19