[1]张紫森,刘良明.周细胞对脓毒症大鼠血管反应性的保护作用[J].第三军医大学学报,2019,41(21):2029-2034.
 ZHANG Zisen,LIU Liangming.Role and mechanism of pericyte in protection of vascular reactivity in septic rats[J].J Third Mil Med Univ,2019,41(21):2029-2034.
点击复制

周细胞对脓毒症大鼠血管反应性的保护作用(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第21期
页码:
2029-2034
栏目:
基础医学
出版日期:
2019-11-15

文章信息/Info

Title:
Role and mechanism of pericyte in protection of vascular reactivity in septic rats
作者:
张紫森刘良明
陆军军医大学(第三军医大学)野战外科研究所第二研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
ZHANG Zisen LIU Liangming

Department 2, Institute of Surgery Research, Army Medical University (Third Military Medical University), Chongqing, 400042, China

关键词:
脓毒症周细胞血管反应性p-MLC20
Keywords:
sepsis pericytes vascular reactivity p-MLC20
分类号:
R322.13; R363.22; R631
文献标志码:
A
摘要:

目的观察周细胞(pericyte, PC)对脓毒症大鼠血管舒缩反应性的保护作用。方法采用盲肠结扎穿孔诱导大鼠脓毒症模型,将SD大鼠(12~14周龄,雌雄各半,体质量180~220 g)按随机单位设计方法分为4组(n=16):正常对照组、脓毒症组、常规治疗组(乳酸林格液体复苏+血管活性药多巴胺+头孢呋辛钠)、PC治疗组(PC 106/只+常规治疗),观察不同处理组大鼠肠系膜微动脉舒缩反应性、肠系膜微血管动静脉流速、肝肾血流量及肠系膜上动脉p-MLC20表达的变化。结果与正常对照组相比,脓毒症大鼠血管反应性明显降低(P<0.01);常规治疗可轻微改善脓毒症血管反应性;PC治疗后,脓毒症大鼠微动脉的舒缩反应性、组织器官的灌注量明显改善(P<0.01),肠系膜微动脉的收缩和舒张反应性分别增加至81.5%和86.9%。机制研究发现PC对脓毒症大鼠血管反应性的保护作用与血管p-MLC20有关(P<0.01)。结论 PC通过改善脓毒症大鼠血管收缩和舒张反应性从而实现了对整体器官的保护作用。

Abstract:

ObjectiveTo determine the protective effects of pericytes (PCs) on the mesenteric vasomotor reactivity in septic rats. MethodsThe sepsis model of rats was established with cecal ligation and puncture (CLP). SD rats (both sexes, 12~14 weeks old, weighing 180 to 220 g) were randomly divided into normal control, sepsis, conventional treatment (CT group), and PCs+CT group, with 16 rats in each group. The rats from the CT group were given conventional fluid resuscitation (Ringer lactate liquid)+dopamine+cefuroxime, and those of the PCs+CT group were given venous injection of 106 cells PCs in addition. The vascular reactivity in mesenteric arteriole, velocity of red blood cells in mesenteric arteriole and venule, amounts of liver and kidney blood flow, and expression level of p-MLC20 in superior mesenteric artery were observed and compared among the groups. ResultsCompared with normal control group, the vascular reactivity was significantly decreased after septic shock (P<0.01). Conventional treatment slightly improved the vascular reactivity. PCs treatment significantly improved vascular hyporeactivity and organ perfusion in rats with septic shock (P<0.01), with constriction and relaxation of mesenteric arteriole increased to 81.5% and 86.9%, respectively. The protective effect of PCs on vascular reactivity was associated with the expression of p-MLC20 (P<0.01). ConclusionPCs protect the vital organ function by improving the vascular constriction and relaxation in septic rats.

参考文献/References:

[1]RABBOLINI D J, ANGE N, WALTERS G D, et al. Systemic capillary leak syndrome: recognition prevents morbidity and mortality[J]. Intern Med J, 2013, 43(10): 1145-1147. DOI:10.1111/imj.12271.
[2]SHARAWY N, LEHMANN C. New directions for sepsis and septic shock research[J]. J Surg Res, 2015, 194(2): 520-527. DOI:10.1016/j.jss.2014.12.014.
[3]AHMAD A A, TABOADA C B, GASSMANN M, et al. Astrocytes and pericytes differentially modulate blood: brain barrier characteristics during development and hypoxic insult[J]. J Cereb Blood Flow Metab, 2011, 31(2): 693-705. DOI:10.1038/jcbfm.2010.148.
[4]GERTZ K, KRONENBERG G, UHLEMANN R, et al. Partial loss of VE-cadherin improves long-term outcome and cerebral blood flow after transient brain ischemia in mice[J]. BMC Neurol, 2016, 16: 144. DOI:10.1186/s12883-016-0670-8.
[5]FERNANDEZ-KLETT F, OFFENHAUSER N, DIRNAGL U, et al. Pericytes in capillaries are contractile in vivo, but arterioles mediate functional hyperemia in the mouse brain[J]. Proc Natl Acad Sci U S A, 2010, 107(51): 22290-22295. DOI:10.1073/pnas.1011321108.
[6]KUTCHER M E, KOLYADA A Y, SURKS H K, et al. Pericyte rho GTPase mediates both pericyte contractile phenotype and capillary endothelial growth state[J]. Am J Pathol, 2007, 171(2): 693-701. DOI:10.2353/ajpath.2007.070102.
[7]HODGE R G, RIDLEY A J. Regulating rho GTPases and their regulators[J]. Nat Rev Mol Cell Biol, 2016, 17(8): 496-510. DOI:10.1038/nrm.2016.67.
[8]DANEMAN R, ZHOU L, KEBEDE A A, et al. Pericytes are required for blood-brain barrier integrity during embryogenesis[J]. Nature, 2010, 468(7323): 562-566. DOI:10.1038/nature09513.
[9]BELL R D, WINKLER E A, SAGARE A P, et al. Pericytes control key neurovascular functions and neuronal phenotype in the adult brain and during brain aging[J]. Neuron, 2010, 68(3): 409-427. DOI:10.1016/j.neuron.2010.09.043.
[10]WINKLER E A, BELL R D, ZLOKOVIC B V. Central nervous system pericytes in health and disease[J]. Nat Neurosci, 2011, 14(11): 1398-1405. DOI:10.1038/nn.2946.
[11]PEPPIATT C M, HOWARTH C, MOBBS P, et al. Bidirectional control of CNS capillary diameter by pericytes[J]. Nature, 2006, 443(7112): 700-704. DOI:10.1038/nature05193.
[12]KAMOUCHI M, KITAZONO T, AGO T, et al. Calcium influx pathways in rat CNS pericytes[J]. Mol Brain Res, 2004, 126(2): 114-120. DOI:10.1016/j.molbrainres.2004.03.008.
[13]CAO C H, GOO J H, LEE-KWON W, et al. Vasa recta pericytes express a strong inward rectifier K+ conductance[J]. Am J Physiol-Regul Integr Comp Physiol, 2006, 290(6): R1601-R1607. DOI:10.1152/ajpregu.00877.2005.
[14]HIRAO M, OKU H, GOTO W, et al. Effects of adenosine on optic nerve head circulation in rabbits[J]. Exp Eye Res, 2004, 79(5): 729-735. DOI:10.1016/j.exer.2004.08.008.
[15]KITAZAWA T, ETO M, WOODSOME T P, et al. Agonists trigger G protein-mediated activation of the CPI-17 inhibitor phosphoprotein of myosin light chain phosphatase to enhance vascular smooth muscle contractility[J]. J Biol Chem, 2000, 275(14): 9897-9900. DOI:10.1074/jbc.275.14.9897.
[16]LI T, LIU L M, LIU J C, et al. Mechanisms of rho kinase regulation of vascular reactivity after hemorrhagic shock in rats[J]. Shock, 2008, 29(1): 65-70. DOI:10.1097/shk.0b013e318063e477.

相似文献/References:

[1]孙慧勤,冯一梅,章容,等.周细胞在创面修复早期血管生成中的分布与意义[J].第三军医大学学报,2009,31(12):1135.
 SUN Hui-qin,FENG Yi-mei,ZHANG Rong,et al.Roles of pericytes in the early angiogenesis of wound healing[J].J Third Mil Med Univ,2009,31(21):1135.

更新日期/Last Update: 2019-11-12