[1]陈影,孙航,娄世锋,等.噬血细胞性淋巴组织细胞增多症患者外周血中高迁移率族蛋白B1的表达及临床意义[J].第三军医大学学报,2019,41(23):2307-2312.
 CHEN Ying,SUN Hang,LOU Shifeng,et al.Expression and clinical significance of high mobility group protein B1 in patients with hemophagocytic lymphohistiocytosis[J].J Third Mil Med Univ,2019,41(23):2307-2312.
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噬血细胞性淋巴组织细胞增多症患者外周血中高迁移率族蛋白B1的表达及临床意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第23期
页码:
2307-2312
栏目:
专题报道
出版日期:
2019-12-15

文章信息/Info

Title:
Expression and clinical significance of high mobility group protein B1 in patients with hemophagocytic lymphohistiocytosis
作者:
陈影孙航娄世锋周慷张颖陈姝罗云沈燕龙贤梨邓建川
400010 重庆,重庆医科大学:附属第二医院血液内科,病毒性肝炎研究所,附属第二医院感染科
 
Author(s):
CHEN Ying1 SUN Hang2 LOU Shifeng1 ZHOU Kang1 ZHANG Ying1 CHEN Shu1 LUO Yun1 SHEN Yan1 LONG Xianli2 DENG Jianchuan1

1Department of Hematology, 2Key Laboratory of Molecular Biology for Infectious Diseases, Institute for Viral Hepatitis, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China

关键词:
噬血细胞性淋巴组织细胞增多症高迁移率族蛋白B1灵敏度特异度
Keywords:
hemophagocytic lymphohistiocytosis high mobility group protein B1 sensitivity specificity
分类号:
R341; R363.25; R551.1
文献标志码:
A
摘要:

目的探讨高迁移率族蛋白B1(high mobility group box 1,HMGB1)在噬血细胞性淋巴组织细胞增多症(hemophagocytic lymphohistiocytosis,HLH)患者体内的表达情况及临床意义。方法收集2018年7月至2019年5月重庆医科大学附属第二医院收治的HLH患者26例相关临床资料,健康志愿者30例作为对照。采用酶联免疫吸附实验(ELISA)检测外周血中HMGB1蛋白水平,实时荧光定量PCR检测外周血中HMGB1 mRNA表达水平,分析HMGB1水平与其他临床指标的相关性以及HMGB1对于诊断HLH的灵敏度、特异度及最佳诊断阈值。结果HMGB1在HLH患者外周血中的浓度较健康志愿者明显升高[(1 992.77±883.74) vs (1 237.49±272.85)ng/mL,P<0.01]。HLH患者组HMGB1 mRNA的相对表达量为(4.56±2.10)倍,较健康志愿者组的(2.34±1.32)倍明显升高(P<0.05)。HLH患者血清中HMGB1含量与红细胞计数(r=-0.514 6,P<0.05)、血红蛋白水平(r=-0.453 6, P<0.05)、血小板计数(r=-0.505 5, P<0.05)均呈负相关。骨髓中发现噬血现象的HLH患者,其HMGB1水平显著高于骨髓中未发现噬血现象的HLH患者[(2 246.27±935.14)vs(1 422.38±317.33)ng/mL,P<0.05]。利用HMGB1诊断HLH的曲线下面积为0.812 2(P<0.05),最佳阈值为1 522 ng/mL,灵敏度为69.23%,特异度为86.67%。结论HMGB1在HLH患者的外周血中高表达,有望用于噬血细胞性淋巴组织细胞增多症的诊断、病情评估和预后分析。

Abstract:

Objective To detect the expression of high mobility group box 1 (HMGB1) in hemophagocytic lymphohistiocytosis (HLH) patients and explore its clinical significance in the disease. MethodsA total of 26 HLH patients admitted in our hospital from July 2018 to May 2019 were recruited, and their clinical data were collected in this study. Thirty healthy volunteers were recruited as normal control. The serum level and mRNA level of HMGB1 in peripheral blood samples were measured by ELISA and quantitative real-time PCR, respectively. The correlation between HMGB1 level and clinical data were analyzed, and the optimal cut-off value, sensitivity and specificity of HMGB1 for the diagnosis of HLH were analyzed. ResultsThe serum HMGB1 level was significantly higher in HLH patients than healthy volunteers (1 992.77±883.74 vs 1 237.49±272.85 ng/mL, P<0.01)]. And the mRNA level of the molecule was also obviously increased in the HLH patients than the healthy control (4.56±2.10 vs 2.34±1.32, P<0.05). The serum level of HMGB1 was negatively correlated with red blood cell count (r=-0.514 6, P<0.05), hemoglobin level (r=-0.453 6, P<0.05) and platelet count (r=-0.505 5, P<0.05). The serum level of HMGB1 was notably higher in the HLH patients with hemophagocytosis in bone marrow than those without (2 246.27±935.14 vs 1 422.38±317.33 ng/mL, P<0.05). The area under the curve (AUC) of HLH diagnosed by HMGB1 was 0.812 2 (P<0.05) and the optimal cut-off value was 1 522 ng/mL, with a sensitivity of 69.23% and specificity of 86.67%. ConclusionHMGB1 is highly expressed in the peripheral blood of HLH patients, and can be used in the diagnosis, evaluation and prognosis of the disease.

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更新日期/Last Update: 2019-12-09