KANG Xia,SONG Hanxiang,GUO Qiaonan.Expression and clinical significance of IBP and p53 in gastric adenocarcinoma[J].J Third Mil Med Univ,2019,41(03):219-226.

IBP与p53蛋白在胃腺癌中的表达及临床意义(/HTML )




Expression and clinical significance of IBP and p53 in gastric adenocarcinoma
KANG Xia SONG Hanxiang GUO Qiaonan

Department of Pathology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China

gastric adenocarcinoma IRF-4 binding protein p53 protein
R341; R730.23; R735.2

目的 探讨干扰素调节因子4结合蛋白(IRF-4 binding protein,IBP)在胃腺癌细胞及临床病理组织中的表达、与p53蛋白表达的相关性及临床意义。方法 通过免疫荧光、Real-time PCR和Western blot检测胃腺癌细胞株MKN-45、SGC-7901中IBP的表达情况;收集2015-2016年在本科确诊的胃腺癌HE切片对应存档蜡块150例,采用免疫组化EnVision法检测并标记IBP在150例胃腺癌标本中的表达,并检测突变型p53蛋白的表达,分析IBP在胃腺癌组织中的表达及其与胃腺癌各临床病理资料因子相应的关系,采用Spearman等级相关分析IBP与p53蛋白表达的相关性。结果 免疫荧光检测显示IBP主要表达在胃腺癌细胞株MKN-45的胞质和胞膜中,主要以胞膜表达为主。Western blot及Real-time PCR均证实胃腺癌细胞株MKN-45、SGC-7901中均有IBP的表达,且在MKN45细胞中的表达高于SGC-7901细胞(P<0.05)。在胃腺癌组织中,IBP的阳性表达率显著高于正常胃黏膜组织(P<0.01);p53蛋白的阳性表达率也显著高于正常胃黏膜组织(P<0.01);IBP阳性表达与病理分级呈正相关(P<0.05),与患者年龄、性别、胃壁浸润深度、TNM分期、Lauren分型及肿瘤部位无相关性(P>0.05);p53蛋白的阳性表达与患者的年龄、性别、病理分级、Lauren分型具有相关性(P<0.05),与患者胃壁浸润深度、TNM分期及肿瘤部位无相关性(P>0.05);在胃腺癌中IBP与p53蛋白表达没有显著的相关关系(r=0.013,P>0.05)。结论 IBP在胃腺癌细胞株及临床病理组织中高表达,主要表达于胞膜、胞质;p53蛋白在胃腺癌临床病理组织中高表达,主要表达于胞核;IBP在胃腺癌组织中高表达与患者病理分级正相关,在胃腺癌组织中IBP与p53蛋白没有明显的相关关系。


Objective To investigate the expression of IRF-4 binding protein (IBP) in gastric adenocarcinoma, and explore its correlation with p53 expression and clinical significance. Methods The expression of IBP in gastric adenocarcinoma cell lines MKN-45 and SGC-7901 was detected with immunofluorescence assay, realtime PCR and Western blotting. Immunohistochemical staining with the aid of EnVision system was used to detect and mark the expression of IBP in 150 cases of gastric adenocarcinoma identified in our department from 2015 to 2016. The expression of mutant p53 protein was also detected. The expression of IBP in gastric adenocarcinoma and its relationship with the clinical pathological data were analyzed, and the correlation between IBP and p53 expression was tested by Spearman grade correlation analysis. Results Immunofluorescence assay showed that IBP was expressed in the cytoplasm and membrane of the MKN-45 cells, mainly in the latter. Western blotting and real-time PCR confirmed that IBP was expressed in the MKN-45 and SGC-7901 cells, and the expression level was significantly higher in the former than the latter cells (P<0.05). The positive expression rate of IBP was obviously higher in the gastric adenocarcinoma tissues than the adjacent normal tissues (P<0.01), so was that of p53 (P<0.01). The positive expression of IBP was positively correlated with pathological grade (P<0.05), but not with the patient’s age, gender, gastric wall invasion depth, TNM stage, Lauren type or tumor site (P>0.05). The positive expression of p53 was correlated with age, sex, pathological grade, and Lauren type (P<0.05), but not with depth of gastric wall invasion, TNM stage or tumor site (P>0.05). No significant correlation was seen between the expression of IBP and p53 in gastric adenocarcinoma (r=0.013, P>0.05). Conclusion IBP is highly expressed in the gastric adenocarcinoma cell lines and gastric adenocarcinoma tissues, mainly in the cytoplasm and membrane, and p53 protein was highly expressed in the tumor tissues, mainly in the nucleus. The high expression of IBP in gastric adenocarcinoma is positively correlated with pathological grade, and there was no significant correlation between their expression.


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更新日期/Last Update: 2019-02-14