[1]余志平,胡明,王洛夫,等.G250纳米抗体靶向的载药纳米微泡抑制肾癌细胞生长的实验研究[J].第三军医大学学报,2018,40(24):2229-2235.
 YU Zhiping,HU Ming,WANG Luofu,et al.Inhibitory effect of G250 nanobody-targeted temsirolimusloaded nanobubbles on growth of renal cancer cells in vitro[J].J Third Mil Med Univ,2018,40(24):2229-2235.
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G250纳米抗体靶向的载药纳米微泡抑制肾癌细胞生长的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第24期
页码:
2229-2235
栏目:
基础医学
出版日期:
2018-12-30

文章信息/Info

Title:
Inhibitory effect of G250 nanobody-targeted temsirolimusloaded nanobubbles on growth of renal cancer cells in vitro
作者:
余志平胡明王洛夫郭燕丽江军兰卫华徐丹朱连华
陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)泌尿外科;陆军军医大学(第三军医大学)第一附属医院超声科
Author(s):
YU Zhiping HU Ming WANG Luofu GUO Yanli JIANG Jun LAN Weihua XU Dan ZHU Lianhua

YU Zhiping, HU Ming, WANG Luofu, GUO Yanli, JIANG Jun, LAN Weihua, XU Dan, ZHU Lianhua

关键词:
靶向载药纳米泡肾透明细胞癌超声辅助药物递送
Keywords:
targeted nanobubbles renal clear cell carcinoma ultrasound-assisted drug delivery systems
分类号:
R73-36;R737.11;R944.9
文献标志码:
A
摘要:

目的   制备G250纳米抗体靶向的携载替西罗莫司的脂质纳米微泡(G250-TEM-NBs),并联合超声靶向纳米微泡破坏(ultrasound targeted nano-bubble destruction, UTMD)辅助药物递送系统实现对肾癌细胞生长的抑制。方法   采用水化薄膜法,联合机械震荡和静电作用制备G250-TEMNBs,检测其粒径、ζ电位、包封率和载药效率。体外观察G250-TEMNBs靶向结合肾癌786-O细胞的能力,通过CCK-8和TUNEL测量G250-TEM-NBs联合UTMD抗肿瘤细胞增殖诱导凋亡效果。结果   G250-TEMNBs平均粒径和ζ电位分别为(399.67±31.01)nm和(23.33±2.62)mV,G250-TEM-NBs与肾癌 786-O细胞的亲和力明显高于TEMNBs(P<0.05),G250-TEM-NBs联合UTMD抗肿瘤细胞增殖能力及诱导肿瘤细胞凋亡作用最强。结论   G250-TEM-NBs联合UTMD能显著抑制肾癌细胞生长,为肾癌靶向治疗提供了一种有效的新方法。

Abstract:

Objective To establish a drug delivery system based on G250 nanobody-targeted lipid nanobubbles carrying temsirolimus (G250-TEM-NBs) combined with ultrasound targeted nanobubble destruction (UTMD) and assess its inhibitory effect on the growth of renal cell carcinoma (RCC) cells in vitro. Methods G250-TEM-NBs were prepared using the hydration membrane method combined with mechanical vibration and electrostatic interaction, and the particle size, zeta potential, entrapment efficiency and drug loading efficiency were assessed. The binding ability of G250-TEM-NBs to renal cancer 786-O cells in vitro was evaluated, and CCK-8 and TUNEL assays were used to evaluate the growth inhibition and apoptosis-inducing effects of G250-TEM-NBs combined with UTMD in 786-O cells. Results The average particle size and zeta potential of G250-TEM-NBs was 399.67±31.01 nm and -23.33±2.62 mV, respectively. The affinity of G250-TEM-NBs to 786-O cells was significantly higher than that of TEMNBs (P<0.05). When combined with UTMD, G250-TEM-NBs showed a strong inhibitory effect on the proliferation and potently induced apoptosis in 786-O cells. Conclusion G250-TEM-NBs combined with UTMD can significantly inhibit the growth of renal cancer cells in vitro, which sheds light on a potentially effective new method for targeted therapy of RCC.

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更新日期/Last Update: 2019-01-02