[1]王文博,古良琪,刘媛,等.2018年成都周边部队感染55型腺病毒分子流行病学溯源[J].第三军医大学学报,2018,40(24):2211-2216.
 WANG Wenbo,GU Liangqi,LIU Yuan,et al.Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area [J].J Third Mil Med Univ,2018,40(24):2211-2216.
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2018年成都周边部队感染55型腺病毒分子流行病学溯源(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第24期
页码:
2211-2216
栏目:
军事医学
出版日期:
2018-12-30

文章信息/Info

Title:
Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area  
作者:
王文博古良琪刘媛杨洋赵秀华周奕帆张雪莲陈锚锚邱薇范泉水胡小兵
西部战区疾病预防控制中心; 西部战区总医院检验科
Author(s):
WANG Wenbo GU Liangqi LIU Yuan YANG Yang ZHAO Xiuhua ZHOU Yifan ZHANG Xuelian CHEN Maomao QIU Wei FAN Quanshui HU Xiaobing

Center for Disease Control and Prevention of Western Theater Command, Chengdu, Sichuan Province, 610021, China; Department of Clinical Laboratory, the General Hospital of Western Theater Command, Chengdu, Sichuan Province, 610083, China

关键词:
55型腺病毒病原分离全基因测序系统发育进化树分子流行病学
Keywords:
adenovirus type 55 pathogen isolation whole genome sequencing phylogenetic tree molecular epidemiology
分类号:
R181.81;R372;R511.8
文献标志码:
A
摘要:

目的    对成都地区驻地部队暴发55型腺病毒进行病原分离、全基因序列及和遗传进化特点等分子流行病学分析,为揭示55型腺病毒的进化起源及制订疾病防控策略提供参考数据。方法    采集55型腺病毒感染者咽拭子标本,采用人喉癌上皮细胞HEp2分离病毒株。利用分片段PCR和测序获取毒株的全基因序列,检索并下载GenBank数据库中55型腺病毒全基因序列,采用生物信息学分析软件-MEGA6.06进行序列比对和系统发育进化树构建。结果    从成都周边驻地部队3起感染疫情中分离出55型腺病毒5株,分别为24-1株、24-2株、1804001株、1806003株、DYN株。采用12片段分段PCR和测序获得了5株病毒全基因序列,GenBank登录号分别为MH256653~MH256657,5株病毒之间序列差异较小,仅有16处碱基变异。与我国报道的第一株55型腺病毒QSDLL株和重庆株(CQ-2903)序列相比,有30处碱基变异。根据系统发育进化树分析结果,新分离的5株HAdV-55毒株均与QS-DLL株相近,与我们之前分离的西藏株(LS89/Tibet/2016)、云南株(KM03/YN/2016)等进化距离相对较远。结论    本研究分析发现2018年成都周边驻地部队流行的55型腺病毒为QSDLL类似株或重庆分离株CQ-2903株的突变株。

Abstract:

ObjectiveTo conduct a molecular epidemiological analysis of 3 human adenovirus type 55 (HAdV-55) outbreaks in the year 2018 in military forces in Chengdu area and obtain epidemiological evidence to identify the evolutionary origin of HAdV55 for formulating disease prevention and control strategies. MethodsThroat swab specimens from HAdV-55-infected individuals were collected for virus isolation via HEp-2 cells. The full-length DNA sequences of the isolated HAdV-55 strains were obtained with segmented PCR and sequencing. The whole genome sequences of HAdV-55 retrieved from the GenBank database were used for sequence alignment and phylogenetic tree construction using bioinformatics analysis software MEGA6.06. ResultsFive strains of HAdV-55, namely 24-1 strain, 24-2 strain, 1804001 strain, 1806003 strain, and DYN strain, were isolated from 3 outbreaks in the military forces stationed around Chengdu City. The whole genome sequences of these 5 HAdV-55 strains (GenBank accession numbers MH256653, MH256654, MH256655, MH256656, and MH256657, respectively) obtained by 12-segment fragmentation PCR sequencing showed only minor variations with mutations of 16 bases. Compared with the sequence of the first isolated HAdV-55 strain (QS-DLL) in China, these strains showed mutations at 30 bases. Phylogenetic tree analysis showed that the 5 newly isolated HAdV-55 strains were similar to the QSDLL strain and the Chongqing strain (CQ-2903), and their evolutionary distances were greater from the previously isolated Tibet strain (LS89/Tibet/2016) and Yunnan strain (KM03/YN/2016). ConclusionThe HAdV-55 strains prevalent in the 2018 outbreaks in the military forces around Chengdu are QS-DLL-like strains or mutant strains of CQ-2903.

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更新日期/Last Update: 2019-01-02