[1]吴悠,殷开宇,李远,等.丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力[J].第三军医大学学报,2018,40(24):2236-2242.
 WU You,YIN Kaiyu,LI Yuan,et al.Propofol inhibits invasion and migration of breast cancer cells via down-regulating transforming growth factor-β1[J].J Third Mil Med Univ,2018,40(24):2236-2242.
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丙泊酚下调TGF-β1抑制乳腺癌细胞迁移和侵袭能力(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第24期
页码:
2236-2242
栏目:
基础医学
出版日期:
2018-12-30

文章信息/Info

Title:
Propofol inhibits invasion and migration of breast cancer cells via down-regulating transforming growth factor-β1
作者:
吴悠殷开宇李远彭明清
重庆医科大学附属永川医院:麻醉科,中心实验室
Author(s):
WU You YIN Kaiyu LI Yuan PENG Mingqing

Department of Anesthesiology, Central Laboratory, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, 402160, China

关键词:
丙泊酚乳腺癌细胞转化生长因子-&beta1肿瘤转移血管拟态形成
Keywords:
propofol breast cancer transforming growth factor-&beta1 metastasis vascular mimicry
分类号:
R73-37;R737.9;R971.2
文献标志码:
A
Abstract:

Objective    To investigate the inhibitory effect of propofol on the migration and invasion of breast cancer cells and the role of transforming growth factor-β1 (TGF-β1) in mediating this effect.  Methods    Cultured breast cancer MCF-7 cells were treated with DMSO, 25 μg/mL propofol, or 25 μg/mL propofol plus 10 ng/mL TGF-β1. Western blotting was used to detect the expression of TGF-β1, MMP-9 and E-cadherin in the cells, and Transwell migration assay, Matrigel invasion assay and vascular mimicry assay were performed to assess the changes in the cell migration, invasion and angiogenesis in response to the treatments.  Results    Compared with the blank control cells, the cells with propofol treatment alone showed significantly decreased expression levels of TGF-β1 (P<0.05) and MMP-9 (P<0.01) and increased expression level of Ecadherin (P<0.05). Compared with the control cells, propofol treatment significantly reduced the number of migrating cells in Transwell migration assay (163.3±8.4 vs 84.7±4.5), the number of invasive cells in Matrigel invasion assay (190.4±8.4 vs 130.7±4.3), and the number of neovascular lumens (108.0±7.6 vs 53.7±5.6) (P<0.01). Compared with the propofol-treated cells, the cells treated with both TGF-β1 and propofol exhibited a significant upregulation of the expression of TGF-β1 (P<0.05) and MMP-9 (P<0.01) and down-regulation of E-cadherin expression (P<0.001), with obviously increased numbers of migrating cells (100.7±4.9 vs 180.3±9.6), invasive cells (57.3±5.7 vs 172.0±10.3), and neovascular lumens (50.7±5.8 vs 107.3±8.6) (P<0.05). Conclusion    Propofol can inhibit the migration and invasion of breast cancer cells in vitro by downregulating TGF-β1.

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更新日期/Last Update: 2019-01-02