[1]孟涛,杨墨,高晔,等.吸入炭黑气溶胶致小鼠肺和外周血的炎性改变及相关性分析[J].第三军医大学学报,2018,40(18):1636-1643.
 MENG Tao,YANG Mo,GAO Ye,et al.Pulmonary and peripheral blood inflammatory responses to black carbon aerosol inhalation in rats and correlation analysis[J].J Third Mil Med Univ,2018,40(18):1636-1643.
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吸入炭黑气溶胶致小鼠肺和外周血的炎性改变及相关性分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第18期
页码:
1636-1643
栏目:
基础医学
出版日期:
2018-09-30

文章信息/Info

Title:
Pulmonary and peripheral blood inflammatory responses to black carbon aerosol inhalation in rats and correlation analysis
作者:
孟涛杨墨高晔叶萌段化伟戴宇飞郑玉新
山西大同大学医学院;中国疾病预防控制中心职业卫生与中毒控制所化学污染与健康安全重点实验室;广州市环境污染与健康风险评价重点实验室;青岛大学公共卫生学院
Author(s):
MENG Tao YANG Mo GAO Ye YE Meng DUAN Huawei DAI Yufei ZHENG Yuxin

Medical School, Shanxi Datong University, Datong, Shanxi Province, 037009; Key Laboratory of Chemical Safety and Health, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, 100050; Guangzhou Key Laboratory of Environmental Pollution and Health Risk Assessment, Guangzhou, Guangdong Province, 510080; School of Public Health, Qingdao University, Qingdao, Shandong Province, 266021, China

关键词:
炭黑纳米级颗粒物吸入染毒急性期反应蛋白细胞因子
Keywords:
black carbon nanoparticles inhalation exposure acute phase reactive proteins cytokines
分类号:
R-332;R135.2;R392.3
文献标志码:
A
摘要:

目的     研究反复吸入炭黑气溶胶致小鼠肺和外周血急性期反应蛋白、炎性细胞因子及免疫细胞的改变,并探讨吸入炭黑后肺和外周血炎性改变的相关性。方法    染毒组将小鼠全身暴露于15.16 mg/m3炭黑气溶胶中6 h/d,分别连续染毒7 d和14 d;恢复组于染毒14 d后恢复14 d,而对照组给予空气。末次染毒24 h或恢复14 d后,收集血液、肺泡灌洗液、肺组织匀浆后进行急性期蛋白、炎性细胞因子及免疫细胞检测。解剖取肺进行病理组织学及超微结构观察。结果与对照组相比,染毒7、14 d组肺组织匀浆、肺泡灌洗液及血清中CRP、SAA、IL-6、IL-8、TNF-α水平均显著高于对照组(P<0.05),肺泡灌洗液及外周血中嗜酸性粒细胞百分比显著升高(P<0.05),单核/巨噬细胞百分比显著降低(P<0.05),且这些炎症变化在恢复期并未得到改善。相关分析结果显示,肺组织和外周血的炎性水平存在较好的相关性,且相关系数r=0.665以上。病理检查可见染毒及恢复组肺组织呈灰黑色,镜下支气管壁及肺泡壁结构紊乱、肺间隔增厚、炎性细胞浸润,肺泡腔和肺间隔可见黑色颗粒和巨噬细胞。电镜结果显示,染毒及恢复组巨噬细胞中可见较多溶酶体,且在溶酶体内存在电子密度与炭黑颗粒一致的黑色颗粒。结论    反复吸入15.16 mg/m3炭黑气溶胶造成小鼠肺组织炭黑颗粒沉积和炎性损伤以及循环系统炎症反应,炭黑吸入后小鼠外周血中炎性生物标志物的变化可以准确地反映肺组织的炎性改变。

Abstract:

Objective    To investigate the changes in acute phase reactive proteins, inflammatory cytokines and immune cells in the lungs and peripheral blood in mice exposed to repeated inhalation of black carbon aerosols and explore the correlation of the inhalation and inflammation in the peripheral blood. MethodsAdult C57BL/6 mice were exposed to black carbon aerosols (15.16 mg/m3) in an inhalation chamber for 6 h per day for 7 or 14 consecutive days, and the mice in the recovery group were exposed for 14 d followed by recovery for another 14 d; the mice in the control group were exposed to filtered air for 6 h per day. At 24 h after the last exposure or after the recovery period, the mice were sacrificed, and the blood samples, bronchoalveolar lavage fluid (BALF) and lung tissues were sampled for analyzing acute phase reactive proteins, inflammatory cytokines and immune cells. The histopathology and ultrastructure of the lung tissue were observed with HE staining and transmission electron microscopy, respectively. ResultsExposure to black carbon aerosols for 7 and 14 d significantly increased the levels of C-reactive protein (CRP), amyloid A protein (SAA), interleukin-6 (IL-6), IL-8, and tumor necrosis factor-α (TNF-α) in the lung tissue homogenate, BALF and serum (P<0.05), and significantly increased eosinophil percentage (P<0.05) and lowered mononuclear cells/macrophage percentage (P<0.05) in the BALF and blood. These inflammatory changes were not significantly improved after the recovery period. Correlation analysis showed a strong correlation in the intensity of inflammatory responses between the lungs and the peripheral blood (correlation coefficients all above 0.665). Pathologically, the lung tissue appeared dark gray in the exposure and recovery groups, showing obvious pathologies in the alveoli and bronchioles (disarrangement of bronchial and alveolar walls, thickness of alveolar septum, and infiltration of inflammatory cells) and the presence of black carbon particles and macrophages; transmission electron microscopy revealed numerous lysosomes containing black carbon particles in the alveolar macrophages.  ConclusionRepeated inhalation of 15.16 mg/m3 black carbon aerosols results in carbon particle deposition, inflammatory injury of the lung tissue, and increased levels of systemic inflammatory responses. The changes in peripheral blood inflammatory biomarkers can accurately reflect the inflammatory levels in the lung tissues after the exposure.
 

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更新日期/Last Update: 2018-09-28