[1]柏萍娟,王如波,曾菲,等.DFMG调节血管新生对APOE-/-小鼠动脉粥样硬化斑块稳定性的影响[J].陆军军医大学学报(原第三军医大学学报),2018,40(17):1554-1560.
 BO Pingjuan,WANG Rubo,ZENG Fei,et al.DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis[J].J Amry Med Univ (J Third Mil Med Univ),2018,40(17):1554-1560.
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DFMG调节血管新生对APOE-/-小鼠动脉粥样硬化斑块稳定性的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第17期
页码:
1554-1560
栏目:
基础医学
出版日期:
2018-09-15

文章信息/Info

Title:
DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis
作者:
柏萍娟王如波曾菲向丽萍向雪萍符晓华张勇
湖南师范大学医学院心血管疾病研究室
Author(s):
BO Pingjuan WANG Rubo ZENG Fei XIANG Liping XIANG Xueping FU Xiaohua ZHANG Yong

Laboratory of Cardiovascular Diseases, Medical College of Hunan Normal University, Changsha, Hunan Province, 410006, China

关键词:
7-二氟亚甲基54&prime二甲烷氧基异黄酮动脉粥样化血管新生TLR4斑块稳定
Keywords:
7-difluoromethoxy-54&primedimethoxygenistein atherosclerosis angiogenesis TLR4 plaque stability
分类号:
R-332; R543.5; R966
文献标志码:
A
摘要:

目的    探讨7-二氟亚甲基-5,4′-二甲烷氧基异黄酮(7-difluoromethoxy-5,4′dimethoxygenistein,DFMG)对载脂蛋白E基因敲除(apolipoprotein E knockout,ApoE-/-)动脉粥样硬化模型小鼠血管新生的影响,及其对动脉粥样硬化斑块稳定性的作用。 方法    将20只动脉粥样硬化模型ApoE-/-小鼠分为4组(每组5只):模型组、溶剂组、DFMG组和洛伐他汀组, 在高脂饲养的同时,DFMG组添加DFMG 10 mg/(kg·d),洛伐他汀组添加洛伐他汀5 mg/(kg·d),溶剂组添加DMSO 10 mg/(kg·d);另取5只C57BL/6小鼠普通饮食喂养作为空白组。喂养16周后,取血清检测小鼠血脂,胸主动脉大体标本油红O染色,组织HE染色检测脂质斑块、Masson染色检测斑块稳定性、免疫组化观察血管新生情况以及Western blot检测TLR4蛋白表达情况。 结果    DFMG降低主动脉粥样斑块与血管腔内径比值(P<0.05),降低血浆血脂LDL、VLDL、TG、CHOL水平(P<0.05),减少胸主动脉油红O染色脂质斑块面积(P<0.05),增加斑块胶原纤维含量(P<0.05),降低胸主动脉VEGF 、vWF和TLR4蛋白表达(P<0.05)。结论    DFMG可抑制动脉粥样硬化ApoE-/-小鼠血管新生,维持小鼠动脉粥样硬化斑块稳定性。

Abstract:

Objective    To investigate the effect of 7-difluoromethoxy-5,4′-dimethoxygenistein (DFMG) on the angiogenesis and plaque stability in the atherosclerosis model of the Apolipoprotein E knockout mice (ApoE-/-).  Methods    A total of 20 ApoE-/- mice were randomly divided into 4 groups (n=5): model group, solvent (DMSO) group, DFMG group, and lovastatin group. And 5 C57BL/6 mice were assigned to the blank control group. The blank control group was fed with normal diet and other 4 groups were fed with highfat diet to induce atherosclerosis model. DFMG of 10 mg/(kg·d) was given to the mice of the DFMG group, and lovastatin 5 mg/(kg·d) to those of the lovastatin group. After 16 weeks, lipid content in the mice serum was measured by automatic biochemical analyzer. The lipid deposition in the gross thoracic aorta was evaluated by oil red O staining. The plaque stability in the thoracic aorta was measured by Masson staining. The angiogenesis was measured by the immunohistochemical staining with VEGF and vWF antibodies. The protein levels of TLR4 in the thoracic aorta were detected by Western blotting. Results    DFMG decreased the ratio of atherosclerotic plaque to intraluminal diameter (P<0.05), reduced the levels of plasma lipids, including LDL, VLDL, TG and CHOL (P<0.05), and attenuated the amount of lipid red stains in the thoracic aorta (P<0.05), increased collagen in the plaque (P<0.05) and lowered the expression of VEGF, vWF and TLR4 in the thoracic aorta (P<0.05). Conclusion    DFMG may inhibit the angiogenesis and maintain the stability of atherosclerotic plaque in the atherosclerotic ApoE-/- mice.

更新日期/Last Update: 2018-09-19