[1]王轶,刘博深,傅丰文,等.特发性弱精子症精浆蛋白差异表达研究[J].第三军医大学学报,2018,40(09):823-829.
 WANG Yi,LIU Boshen,FU Fengwen,et al.Differential proteomics analysis of seminal plasma from idiopathic asthenospermia[J].J Third Mil Med Univ,2018,40(09):823-829.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第09期
页码:
823-829
栏目:
临床医学
出版日期:
2018-05-15

文章信息/Info

Title:
Differential proteomics analysis of seminal plasma from idiopathic asthenospermia
作者:
王轶刘博深傅丰文张昊昱靳风烁孙中义
陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)泌尿外科
Author(s):
WANG Yi LIU Boshen FU Fengwen ZHANG Haoyu JIN Fengshuo SUN Zhongyi

Department of Urology, Institute of Surgery Research, Third Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China

关键词:
弱精子症精浆差异蛋白质组等重同位素多标签相对定量蛋白质组学
Keywords:
asthenospermia differential proteomics of seminal plasma isobaric tags for relative and absolute quantitation
分类号:
R321.1; R394.3; R698.2
文献标志码:
A
摘要:

目的    探讨精浆蛋白在成年男性特发性弱精子症中的差异表达情况。方法    纳入2017年1-5月我院泌尿外科门诊特发性弱精子症患者15例作为试验组,年龄24~47岁。同时,纳入精液正常成年男性15例作为对照组,年龄21~44岁。用等重同位素多标签相对定量蛋白质组学(isobaric tags for relative and absolute quantitation,iTRAQ)的方法研究成年男性正常精浆蛋白以及特发性弱精子症患者的精浆蛋白表达情况。结果    共发现精浆中2 784个蛋白质。其中,与正常组相比,特发性弱精子症患者精浆中表达上调蛋白数为14个,精浆中表达下调蛋白数为79个(表达差异倍数>1.2倍,且P<0.05)。经斑点印迹技术验证蛋白INF2表达水平与iTRAQ结果一致。经GO(gene ontology)蛋白功能注释及富集分析,特发性弱精子症精浆中差异蛋白功能主要包括调节钙离子跨膜转运、胞浆内转运以及螯合、蝶呤钼辅因子合成及代谢、调节铜离子跨膜转运等。经KEGG蛋白通路注释及富集分析,特发性弱精子症精浆中差异蛋白主要富集于叶酸生物合成、金黄色葡萄球菌感染等通路。结论     特发性弱精子症患者精浆蛋白同正常成年男性精浆蛋白存在差异,其可能是引起特发性弱精子症的原因。

Abstract:

Objective     To explore the differential expression profile of the seminal plasma proteome in idiopathic asthenospermia. Methods    Fifteen patients (24~47 years old) with identified idiopathic asthenospermia admitted in our outpatient department from January to May 2017 were enrolled as the experimental group. Fifteen adult males (21~44 years old) with normal semen were enrolled as the control group. Isobaric tags for relative and absolute quantitation (iTRAQ) labeling was used to determine the expression of seminal plasma proteins in the 2 groups of males. Results    There were 2 784 seminal plasma proteins found in this study. Compared with the normal group, there were 14 proteins up-regulated and 79 down-regulated in seminal plasma of idiopathic asthenospermia patients (the difference was more than 1.2 times, P<0.05). Dot blotting confirmed that INF2 expression level was consistent with the result of iTRAQ. According to the Gene Ontology (GO) annotation and enrichment analysis, the differential proteins were mainly involved in the regulation of calcium ion transmembrane transport, calcium ion intracellular transport and calcium ion sequestering, molybdopterin cofactor metabolic/biosynthetic process, and copper ion transmenbrane transport. According to the results of KEGG protein pathway annotation and enrichment analysis, the differential proteins were mainly involved in folate biosynthesis, Staphylococcus aureus infection and other pathways. Conclusion    There are proteomic differences in seminal plasma between idiopathic asthenospermia and normal semen, which may be the cause of idiopathic asthenospermia.
 

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更新日期/Last Update: 2018-05-11