[1]王文凯,曾昕,左睿,等.小鼠股骨骨缺损诱导膜模型的构建与评估[J].第三军医大学学报,2018,40(09):805-809.
 WANG Wenkai,ZENG Xin,ZUO Rui,et al.Establishment and evaluation of mouse model of induced membrane by femur bone defect[J].J Third Mil Med Univ,2018,40(09):805-809.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第09期
页码:
805-809
栏目:
基础医学
出版日期:
2018-05-15

文章信息/Info

Title:
Establishment and evaluation of mouse model of induced membrane by femur bone defect
作者:
王文凯曾昕左睿孙超王雁秋柯松李杰周跃
重庆,陆军军医大学(第三军医大学)第二附属医院骨科;重庆市药品技术审评认证中心
Author(s):
WANG Wenkai ZENG Xin ZUO Rui SUN Chao WANG Yanqiu KE Song LI Jie ZHOU Yue

Department of Orthopaedics, Second Affiliated Hospital, Amy Military University(Third Military Medical University),Chongqing, 400037; Food and Drug Administration, Chongqing, 400014, China

关键词:
股骨骨缺损诱导膜Masqulet技术小鼠动物模型
Keywords:
femur defect induced membrane Masquelet technique mice animal model
分类号:
R-332; R322.71; R681.05
文献标志码:
A
摘要:

目的    建立标准化成年小鼠股骨骨缺损诱导膜模型。方法    取18只C57BL/6品系的小鼠,6只测量小鼠股骨解剖参数,12只小鼠制备长度为3 mm的股骨干中段骨缺损。应用骨水泥髓内钉固定股骨,术后1周X线片验证内固定效果;术后4周获取骨水泥表面诱导膜组织,运用组织学染色观察诱导膜组织和正常骨膜组织的差异。结果    术后1周X线片证实1只小鼠出现内固定失效;术后4周,HE染色证实11只小鼠骨水泥周围均有典型诱导膜形成,建模成功率91.7%。结论    通过PMMA骨水泥髓内钉系统成功制备了成年小鼠股骨骨缺损诱导膜模型,4周后诱导膜形成良好,组织学证实其为小鼠诱导膜组织。

Abstract:

Objective    To establish a simple, reproductive and standardized induced membrane model by femur bone defect in mice. Methods    Eighteen C57BL/6 mice were used in this study. Six mice were employed for anatomic parameters of the femur. Segmental defect of 3 mm in size were inflicted in the right femur of the rest 12 mice. An intramedullary nail with cement spacer was placed in the defect to offer the stability of femur. The fixation effect was evaluated at 1 week postoperatively by X-ray filming. In 4 weeks after operation, the induced membrane surrounding cement spacer was collected, and HE staining was used to observe the pathological differences between the induced membrane and normal periosteum. Results    X-ray film demonstrated internal fixation fail was observed in 1 mouse. Induced membrane was formed around the cement spacer in the other 11 mice. HE staining confirmed the characteristics of induced membrane. Satisfactory models were created in 91.7% experimental mice. Conclusion     An adult mouse model of induced membrane is successfully established by femur defect followed by intramedullary nail fixation with cement spacer system. Mature induced membrane could be obtained at 4 week after surgery in mice. This model of induced membrane is of high repeatability and confirmed by histology.

参考文献/References:

[1]OLESEN U K, ECKARDT H, BOSEMARK P, et al. The masquelet technique of induced membrane for healing of bone defects. a review of 8 cases[J]. Injury, 2015, 46(Suppl 8): S44-S47. DOI:10.1016/S00201383(15)300541.
[2]GIANNOUDIS P V, FAOUR O, GOFF T, et al. Masquelet technique for the treatment of bone defects: tipstricks and future directions[J]. Injury, 2011, 42(6): 591-598. DOI:10. 1016/j.injury.2011.03.036.
[3]MASQUELET A C, FITOUSSI F, BEGUE T, et al. Reconstruction of the long bones by the induced membrane and spongy autograft[J]. Ann Chir Plast Esthet, 2000, 45(3): 346-353.
[4]APARD T, BIGORRE N, CRONIER P, et al. Twostage reconstruction of posttraumatic segmental tibia bone loss with nailing[J]. Orthop Traumatol Surg Res, 2010, 96(5): 549-553. DOI:10.1016/j.otsr.2010.02.010.
[5]PELISSIER P, MASQUELET A C, BAREILLE R, et al. Induced membranes secrete growth factors including vascular and osteoinductive factors and could stimulate bone regeneration[J]. J Orthop Res, 2004, 22(1): 73-79. DOI:10.1016/S07360266(03)001657.
[6]HULSARTBILLSTRM G, BERGMAN K, ANDERSSON B, et al. A unicortical femoral defect model in the rat: evaluation using injectable hyaluronan hydrogel as a carrier for bone morphogenetic protein2[J]. J Tissue Eng Regen Med, 2015, 9(7): 799-807. DOI:10.1002/term.1655.
[7]GRUBER H E, ODE G, HOELSCHER G, et al. Osteogenic, stem cell and molecular characterisation of the human induced membrane from extremity bone defects[J]. Bone Joint Res, 2016, 5(4): 106-115. DOI:10.1302/20463758.54.2000483.
[8]HENRICH D, SEEBACH C, NAU C, et al. Establishment and characterization of the Masquelet induced membrane technique in a rat femur criticalsized defect model[J]. J Tissue Eng Regen Med, 2016, 10(10): E382-E396. DOI:10.1002/term.1826.
[9]DIMITRIOU R, MATALIOTAKIS G I, ANGOULES A G, et al. Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review [J]. Injury,2011,42(Suppl 2): S3-S15. DOI:10.1016/j.injury.2011.06.015.
[10]MORELLI I, DRAGO L, GEORGE D A, et al. Masquelet technique: myth or reality? a systematic review and metaanalysis[J]. Injury, 2016, 47(Suppl 6): S68-S76. DOI: 10.1016/S00201383(16)308427.
[11]MOGHADDAM A, ZIETZSCHMANN S, BRUCKNER T, et al. Treatment of atrophic tibia nonunions according to ‘diamond concept’: results of one and twostep treatment[J]. Injury, 2015, 46(Suppl 4): S39-S50. DOI:10.1016/S00201383(15)300176.
[12]GRUBER H E, RILEY F E, HOELSCHER G L, et al. Osteogenic and chondrogenic potential of biomembrane cells from the PMMAsegmental defect rat model[J]. J Orthop Res, 2012, 30(8): 1198-1212. DOI:10.1002/jor.22047.
[13]BOSEMARK P, PERDIKOURI C, PELKONEN M, et al. The masquelet induced membrane technique with BMP and a synthetic scaffold can heal a rat femoral critical size defect[J]. J Orthop Res, 2015, 33(4): 488-495. DOI:10.1002/jor.22815.
[14]MANASSERO M, DECAMBRON A, HUU THONG B T, et al. Establishment of a segmental femoral criticalsize defect model in mice stabilized by plate osteosynthesis[J]. J Vis Exp,2016(116): 52940.DOI:10.3791/52940.
[15]WANG X H,WEI F D ,LUO F, et al. Induction of granulation tissue for the secretion of growth factors and the promotion of bone defect repair[J]. J Orthop Surg Res,2015,10:147.DOI: 10.1186/s1301801502874.
[16]GRUBER H E, GETTYS F K, MONTIJO H E, et al. Genomewide molecular and biologic characterization of biomembrane formation adjacent to a methacrylate spacer in the rat femoral segmental defect model[J]. J Orthop Trauma, 2013, 27(5): 290-297. DOI:10.1097/BOT.0b013e318 2691288.
[17]MONTIJO H E,KELLAM J F,GETTYS F K,et al.Utilization of the AO LockingRatNail in a novel rat femur critical defect model[J].J Invest Surg,2012,25(6):381-386.DOI:10.3109/ 08941939.2012.655370.
[18]靳慧勇,侯天勇,罗飞,等.小鼠股骨临界骨缺损模型的构建及评估[J].第三军医大学学报,2011,33(22):2331-2334.
JIN H Y,HOU T Y,LUO F,et al.Establishment and evaluation of mouse femur critical bone defect model[J].J Third Mil Med Univ,2011,33(22):2331-2334.

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 Yu Shengpeng,Fu Jingsu,Li Wei,et al.Clinical observation of Masquelet technique in treatment ofosteomyelitis bone defects of long bones: report of 25 cases[J].J Third Mil Med Univ,2014,36(09):1630.

更新日期/Last Update: 2018-05-11