[1]林夏,朱元鑫,付航玮,等.Hsa_circ_0000711在人肝癌细胞系中的功能研究[J].第三军医大学学报,2018,40(09):788-794.
 LIN Xia,ZHU Yuanxin,FU Hangwei,et al.Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines[J].J Third Mil Med Univ,2018,40(09):788-794.
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Hsa_circ_0000711在人肝癌细胞系中的功能研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第09期
页码:
788-794
栏目:
基础医学
出版日期:
2018-05-15

文章信息/Info

Title:
Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines
作者:
林夏朱元鑫付航玮李光耀陈平
陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)肝胆外科
Author(s):
LIN Xia ZHU Yuanxin FU Hangwei LI Guangyao CHEN Ping

Department of Hepatobiliary Surgery, Institute of Surgery Research, Third Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China
 

关键词:
肝癌hsa_circ_0000711细胞周期细胞凋亡细胞增殖
Keywords:
hepatocellular carcinoma Hsa_circ_0000711 cell cycle cell apoptosis cell proliferation
分类号:
R394.3; R730.23; R735.7
文献标志码:
A
摘要:

目的    研究环状RNA hsa_circ_0000711在人肝癌细胞系中的表达以及对肝癌细胞生物学功能的影响。方法   利用RT-qPCR检测环状RNA hsa_circ_0000711在肝癌细胞系的表达;通过小干扰RNA特异性敲低hsa_circ_0000711的表达;通过CCK-8实验检测肝癌细胞系的增殖情况;利用流式细胞技术检测细胞周期及细胞凋亡。Western blot检测下调hsa_circ_0000711后Cyclin D1、CDK4、cleavaed Caspase 3及Bcl-2蛋白的表达水平。结果    Hsa_circ_0000711在肝癌细胞HepG2和SMMC-7721中明显高表达(P<0.05)。与对照组相比,hsa_circ_0000711干扰组的肝癌细胞增殖能力显著降低(P<0.05),处于G1期细胞增多(P<0.05),且凋亡细胞显著增加(P<0.05)。干扰hsa_circ_0000711的表达后,肝癌细胞中Cyclin D1、CDK4和Bcl-2的蛋白表达量显著降低(P<0.05),cleavaed Caspase 3蛋白表达量显著升高(P<0.05)。结论     Hsa_circ_0000711可能通过调控细胞周期及凋亡影响肝癌细胞的增殖。

Abstract:

Objective    To explore the expression profile of circular RNA (circRNA), hsa_circ_0000711, in hepatocellular carcinoma (HCC) cell lines and determine the effect of hsa_circ_0000711 on the biological behaviors of HCC cells. Methods    The expression level of Hsa_circ_0000711 were detected by real-time polymerase chain reaction assay (RT-qPCR) in HCC cell lines HepG2 and SMMC-7721 and human live cell line L02. Small RNA interference was used to knock down the expression of hsa_circ_0000711. Then CCK-8 assay was employed to detect the proliferation, and flow cytometry for cell cycle and apoptosis. The protein levels of Cyclin D1, CDK4, cleaved Caspase 3 and Bcl-2 were measured by Western blotting. Results    Hsa_circ_0000711 was more strongly expressed in the HepG2 and SMMC-7721 cells than the L02 cells (P<0.05). Down-regulation of hsa_circ_0000711 significantly suppressed the growth rate of HepG2 and SMMC-7721 cells (P<0.05), arrested the cells at cycle G1 (P<0.05), and  promoted cell apoptosis (P<0.05). After hsa_circ_0000711 interference, the protein expression levels of Cyclin D1, CDK4 and Bcl-2 were decreased notably (P<0.05), but that of cleaved Caspase 3 was increased (P<0.05). Conclusion    Hsa_circ_0000711 may affect HCC cell proliferation by regulating cell cycle and cell apoptosis.

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更新日期/Last Update: 2018-05-11