[1]向荣凤,戴青,周艳,等.HPLC-MS/MS测定大鼠血浆中人参皂苷Rg3的浓度[J].第三军医大学学报,2018,40(12):1073-1078.
 XIANG Rongfeng,DAI Qing,ZHOU Yan,et al.Determination of ginsenoside Rg3 in rat plasma using highperformance liquid chromatographytandem mass spectrometry [J].J Third Mil Med Univ,2018,40(12):1073-1078.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第12期
页码:
1073-1078
栏目:
基础医学
出版日期:
2018-06-30

文章信息/Info

Title:
Determination of ginsenoside Rg3 in rat plasma using highperformance liquid chromatographytandem mass spectrometry
 
作者:
向荣凤戴青周艳喻明洁来小丹
陆军军医大学(第三军医大学)第一附属医院药剂科
Author(s):
XIANG Rongfeng DAI Qing ZHOU Yan YU Mingjie LAI Xiaodan

Department of Pharmacy, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
人参皂苷Rg3血浆高效液相色谱串联质谱药代动力学
Keywords:
ginsenoside Rg3 plasma high-performance liquid chromatography-tandem mass spectrometry pharmacokinetics  
分类号:
R285.5;R965.2;R969.1
文献标志码:
A
摘要:

目的建立高效液相色谱串联质谱(HPLCMS/MS)法测定大鼠血浆中人参皂苷Rg3的浓度,用于大鼠体内人参皂苷Rg3的药代动力学研究。方法血浆样品经乙酸乙酯液液提取。色谱柱为Inertsil ODS3(2.1 mm×50 mm,5 μm),流动相:乙腈:10 mmol/L乙酸铵水溶液(含0.1%甲酸,90∶10);流速:0.4 mL/min;ESI离子源,负离子模式监测。6只大鼠灌胃给予人参皂苷Rg3 20 mg/kg后按预定时间点眼眶采血。采用DAS 3.3.1软件统计其药代动力学参数。结果血浆中人参皂苷Rg3的线性范围为2~400 ng/mL,日内、日间精密度及基质效应RSD均小于15%,主要的药代动力学参数:AUC0t为(821.659±170.125)ng·h/mL,AUC0∞为(912.468±190.653)ng·h/mL,Cmax为(138.803±28.997)ng/mL,t1/2为(2.803±0.263)h。结论建立的方法快速、灵敏、准确。适用于大鼠血浆中人参皂苷Rg3浓度的测定。

Abstract:

ObjectiveTo establish a high-performance liquid chromatographytandem mass spectrometry (HPLCMS/MS) system for determination of ginsenoside Rg3 content in rat plasma. MethodsVenous blood samples were collected from the orbit of 6 rats at different time points after intragastric administration of 20 mg/kg ginsenosides Rg3. Plasma was isolated and the samples were extracted with ethyl acetate, followed by chromatographic separation using a C18 column (2.1 mm×50 mm, 5μm) with acetonitrileammonium acetate (10 mmol/L, containing 0.1% formic acid) (90∶10) as the mobile phase at a flow rate 0.4 mL/min. The detection was accomplished with the electrospray ionization source operated in the negative ionization mode. The pharmacokinetic parameters were computed using DAS 3.3.1 pharmacokinetic software. ResultsThe linear calibration curves of ginsenoside Rg3 in rat plasma were obtained within the concentration range of 2~400 ng/mL. The inter-and intra-batch RSDs and the matrix effect of ginsenoside Rg3 detection were all below 15%. Analysis of the pharmacokinetic parameters showed that in rats, ginsenoside Rg3 had an AUC0-t of 821.659±170.125 ng·h/mL, an AUC0-∞ of 912.468±190.653 ng·h/mL, a Cmax of 138.803±28.997 ng/mL, and a t1/2 of 2.803±0.263 h. ConclusionThis HPLC-MS/MS system allows rapid, sensitive and accurate determination of ginsenoside Rg3 in rat plasma.

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更新日期/Last Update: 2018-07-03