[1]向娟,益磋,高顺记,等.诊断超声VFLASH技术增强大鼠急性心肌缺血左心射血功能的实验研究[J].第三军医大学学报,2018,40(09):774-779.
 XIANG Juan,YI Cuo,GAO Shunji,et al.Diagnostic ultrasound VFLASH improves left ventricular ejection function in rats with acute myocardial ischemia[J].J Third Mil Med Univ,2018,40(09):774-779.
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诊断超声VFLASH技术增强大鼠急性心肌缺血左心射血功能的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第09期
页码:
774-779
栏目:
基础医学
出版日期:
2018-05-15

文章信息/Info

Title:
Diagnostic ultrasound VFLASH improves left ventricular ejection function in rats with acute myocardial ischemia
作者:
向娟益磋高顺记刘政
陆军军医大学(第三军医大学)第二附属医院超声科;430070 武汉,中国人民解放军武汉总医院超声科
Author(s):
XIANG Juan YI Cuo GAO Shunji LIU Zheng

Department of Ultrasonography, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037; Department of Ultrasonography, Wuhan General Hospital of PLA, Wuhan, Hubei Province, 430070, China

关键词:
急性心肌缺血诊断超声微泡血管效应大鼠
Keywords:
acute myocardial ischemia diagnostic ultrasound microbubble vascular effect rats
分类号:
R-332; R445.1; R542.2
文献标志码:
A
摘要:

目的    研究诊断超声(diagnostic ultrasound,DUS)VFLASH技术对大鼠急性心肌缺血模型左室射血功能的治疗改善作用。方法    6~8周龄健康雄性SD大鼠70只,用随机数字表法分为5组:高机械指数组(HMI,MI=1.4,n=11)、低机械指数组(LMI,MI=0.7,n=17)、HMI联合微泡(MB)组(HMB,n=15)、LMI联合微泡组(LMB,n=16)和对照组(n=11)。结扎冠状动脉前降支建立大鼠急性心肌缺血模型后,分别于24、72、96 h按照分组实施超声治疗,28 d行心脏射血分数(EF)超声检测。超声治疗参数为:频率4 MHz,脉冲重复频率20 Hz,脉冲宽度在MI=1.4时为5个周期、MI=0.7时为18个周期,脉冲持续时间1.2 s,脉冲间隔时间2 s,持续时间1 200 s。记录各组鼠的生存状态,以28 d为终止目标,比较各组大鼠存活率。术后第28天比较各组鼠左室射血功能(EF值),并取各组鼠心脏组织HE染色观察心肌组织变化。 结果    ①存活率:HMI组72%,LMI组47%,HMB组20%,LMB组50%,对照组82%,仅HMI组高于HMB组,差异具有统计学意义(P<0.05),其余各组之间差异无统计学意义(P>0.05)。②EF:HMI组(87.71±4.69)%,LMI组(78.04±7.35)%,HMB组(75.09±9.47)%,LMB组(76.43±6.83)%,对照组(64.97±9.37)%,单纯超声组(即HMI和LMI组)和LMB组的EF值均明显高于对照组,HMI组高于LMI组,差异均具有统计学意义(P<0.05),LMI与LMB之间差异无统计学意义(P>0.05)。③对照组心肌组织大片坏死、纤维化,各治疗组纤维化面积无明显差异。结论    超声辐照VFLASH技术能够有效改善急性心肌缺血大鼠的心功能,并且单纯高机械指数超声效果较好,更安全。

Abstract:

Objective    To investigate the improvement of cardiac function in acute myocardial ischemia rats by diagnostic ultrasound (DUS) combined with VFLASH (a software which can manipulate microbubble cavitation). Methods    A total of 70 healthy male SD rats (6~8 weeks old) were randomized into 5 groups: high mechanical index group (HMI, MI=1.4, n=11), low mechanical index group (LMI, MI=0.7, n=17), HMI combined with microbubble (MB) group (HMB, n=15), LMI combined with MB group (LMB, n=16), and control group (n=11). Acute myocardial ischemia model was established by ligation of anterior descending coronary artery. Ultrasound treatment was performed in corresponding groups in 24, 72 and 96 h after model establishment. The treatment parameters are as follows: frequency 4 MHz, pulse repeated frequency (PRF) 20 Hz, pulse length (PL) 5 cycles for MI=1.4 groups and 18 cycles for MI=0.7 groups, pulse time 1.2 s, pulse interval time 2 s, duration 1 200 s. The survival status of rats was recorded, and the survival rate was compared among the 5 groups at the end of experiment (28 d). At the 28th day, the rats underwent ultrasonography for cardiac ejection fraction (EF), and the rat heart tissue was collected for pathological changes by HE staining. Results    ① The survival rate was 72% in the HMI group, 20% in the LMB group, 50% in the LMB group, and 82% in the control. The rate of the HMI group was significantly higher than the HMB group (P<0.05), but no difference was seen in other groups (P>0.05). ② The average EF value was (87.71±4.69)% in the HMI group, (78.04±7.35)% in the LMI group, (75.09±9.47)% in the HMB group, (76.43±6.83)% in the LMB group, and (64.97±9.37)% in the control group. The values were significantly higher in the simple ultrasound groups (HMI and LMI groups) and LMB group than the control, and that of HMI higher than that of the LMI groups, with obvious differences (P<0.05). But there were no differences in the values between the LMI and LMB groups (P>0.05). ③ HE staining displayed that the myocardial tissues were necrotic and fibrotic in the control group, but there was no significant difference in fibrotic area in the treatment groups. Conclusion    Ultrasound irradiation in combination with VFLASH can effectively improve the heart function of acute myocardial ischemia rats, with better pure HMI and good safety.

参考文献/References:

[1]梁承院.急性心肌梗死患者接受介入治疗和溶栓治疗的效果对比[J]. 中外医学研究,2017,15(6):67-68. DOI: 10.14033/j.cnki.cfmr.2017.6.073.
LIANG C Y.Comparison of the effect of interventional therapy and thrombolytic therapy in patients with acute myocardial infarction[J].Chin Foreign Med Res, 2017,15(6):67-68. DOI: 10.14033/j.cnki.cfmr.2017.6.073.
[2]SHINDO T, ITO K, OGATA T, et al. Lowintensity pulsed ultrasound enhances angiogenesis and ameliorates left ventricular dysfunction in a mouse model of acute myocardial infarction[J]. Arterioscler Thromb Vasc Biol, 2016, 36(6):1220-1229.DOI: 10.1161/ATVBAHA.115.306477.
[3]HANAWA K, ITO K, AIZAWA K, et al. Lowintensity pulsed ultrasound induces angiogenesis and ameliorates left ventricular dysfunction in a porcine model of chronic myocardial ischemia[J]. PLoS ONE, 2014, 9(8):e104863. DOI:10.1371/journal.pone.0104863.
[4]乔学研, 陈重, 益磋, 等. 诊断超声联合微泡对兔VX2肿瘤的血流增强效应[J]. 临床超声医学杂志, 2017,19(4):217-221.DOI: 10.3969/j.issn.10086978.2017.04.001.
QIAO X Y, CHEN Z, YI C, et al.Vascular effect of rabbit VX2 tumor induced by diagnostic ultrasound with microbubbles[J]. J Clin Ultrasonic Med, 2017,19(4):217-221. DOI: 10.3969/j.issn.10086978.2017.04.001. 
[5]董小小, 高文宏, 王菲, 等. 低声压超声调控微泡空化对大鼠Walker256肿瘤血管效应的初步研究[J]. 第三军医大学学报, 2016,38(8): 811-816. DOI: 10.16016/j.10005404.201509158.
DONG X X, GAO W H, WANG F,et al.Vascular effect of rat Walker256 tumor induced by lowpressureamplitude ultrasound with microbubbles[J]. J Third Mil Med Univ, 2016,38(8): 811-816. DOI: 10.16016/j.10005404.201509158.
[6]赵利培, 杨廷桐, 刘玲玲, 等. 在自然通气状态下和机械通气状态下制作SD大鼠急性心肌梗死模型的效果对比[J]. 当代医药论丛, 2014,12(1): 103-104.DOI: 10.3969/j.issn.20957629.2014.01.106.
ZHAO L P, YANG T T, LIU L L, et al.Comparison of the effect of SD rat model of acute myocardial infarction under the condition of natural ventilation and mechanical ventilation[J]. Contemp Med Forum, 2014,12(1):103-104. DOI: 10.3969/j.issn.20957629.2014.01.106. 
[7]杨涛涛, 肖颖, 奚赛飞, 等.大鼠心肌梗死模型构建和评价方法的改良[J]. 中国比较医学杂志, 2014,24(2): 46-51. DOI:10.3969.j.issn.1671.7856.2014.002.011.
YANG T T, XIAO Y, XI S F, et al.Improvement of the methods of establishing and evaluating rat models of myocardial infarction[J].  Chin J Comparative Med, 2014,24(2): 46-51. DOI:10.3969.j.issn.1671.7856.2014.002.011.
[8]BAKER K G, ROBERTSON V J, DUCK F A. A review of therapeutic ultrasound: biophysical effects[J]. Phys Ther, 2001, 81(7): 1351-1358. DOI: 10.1097/JWH.0b013e 31820089ba.
[9]BELCIK J T, MOTT B H, XIE A,et al. Augmentation of limb perfusion and reversal of tissue ischemia produced by ultrasoundmediated microbubble cavitation[J]. Circ Cardiovasc Imaging, 2015, 8(4):e002979. DOI: 10.1161/CIRCIMAGING.114.002979.
[10]BELCIK J T,DAVIDSON B P,XIE A,et al. Augmentation of muscle blood flow by ultrasound cavitation is mediated by ATP and purinergic signaling[J]. Circulation, 2017, 135(13):1240-1252. DOI: 10.1161/CIRCULATIONAHA.116.024826.
[11]沈丹萍, 戈东辉, 陈显达, 等.bFGF通过促进心脏血管新生改善心肌梗死后小鼠心脏重构[J]. 中国病理生理杂志, 2018,34(1):47-51. DOI: 10.3969/j.issn.10004718.2018.01.008.
SHEN D P, GE D H, CHEN X D, et al.Effect of bFGF on promoting angiogenesis in infarct area and improving myocardial fibrosis in mouse myocardial infarction model[J]. Chin J Pathophysiol, 2018,34(1):47-51. DOI: 10.3969/j.issn.10004718.2018.01.008.
[12]XIE F,GAO S,WU J, et al. Diagnostic ultrasound induced inertial cavitation to noninvasively restore coronary and microvascular flow in acute myocardial infarction[J]. PLoS ONE, 2013, 8(7): e69780. DOI: 10.1371/journal.pone.0069780.
[13]卓莉莎, 高云华, 徐亚丽, 等. 大鼠心肌梗死模型的建立与评价[J].临床超声医学杂志, 2009,11(9): 577-580.DOI: 10.3969/j.issn.10086978.2009.09.001.
ZHUO L S, GAO Y H, XU Y L, et al.Establishment and evaluation of rat model of myocardial infarction[J]. J Ultrasound in Clin Med, 2009,11(9):577-580. DOI: 10.3969/j.issn.10086978.2009.09.001.
[14]沈智勇, 申锷, 胡兵. 低频超声的空化效应及其应用研究进展[J]. 中国医学影像学杂志, 2013(6): 468-470,474. DOI:10.3969/j.issn.10055185.2013.06.019.
SHEN Z Y, SHEN E, HU B. The cavitation effect of low frequency ultrasound and its application research progress[J].Chin J Med Imaging, 2013(6): 468-470,474. DOI: 10.3969/j.issn.10055185.2013.06.019.
[15]刘德英,刘政,李秋颖,等. 超声造影显像模式对实验兔心肌血管通透性的影响[J]. 临床超声医学杂志, 2006, 8(10):577-580. DOI: 10.3969/j.issn.10086978.2006.10.001.
LIU D Y, LIU Z, LI Q Y, et al.Impact of imaging modalities on myocardial permeability in contrast echocardiography[J]. J Ultrasound in Clin Med, 2006, 8(10):577-580. DOI: 10.3969/j.issn.10086978.2006.10.001.

更新日期/Last Update: 2018-05-11