[1]叶喜坤,秦玉,冉莉,等.白藜芦醇通过激活PKA-PLIN2通路促进HepG2细胞脂滴脂解[J].陆军军医大学学报(原第三军医大学学报),2017,39(24):2345-2351.
 YE Xikun,QIN Yu,RAN Li,et al.Resveratrol improves lipid droplet lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2017,39(24):2345-2351.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第24期
页码:
2345-2351
栏目:
基础医学
出版日期:
2017-12-30

文章信息/Info

Title:
Resveratrol improves lipid droplet lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells
作者:
叶喜坤秦玉冉莉周蕊易龙糜漫天
第三军医大学军事预防医学院营养与食品卫生学教研室,营养与食品安全研究中心,重庆市医学营养研究中心,重庆市营养与食品安全重点实验室
Author(s):
YE Xikun QIN Yu RAN Li ZHOU Rui YI Long MI Mantian

Department for Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Chongqing Key Laboratory of Nutrition and Food Safety, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
白藜芦醇HepG2细胞围脂滴蛋白2脂滴
Keywords:
resveratrol HepG2 cellsperilipin-2 lipid droplets
分类号:
R282.71;R285.1;R575.5
文献标志码:
A
摘要:

目的    明确围脂滴蛋白2(perilipinin-2,PLIN2)在白藜芦醇(resveratrol,RSV)改善脂肪变性HepG2细胞脂滴代谢中的作用机制。方法    0.2 mmol/L棕榈酸处理HepG2细胞24 h,建立肝细胞脂肪变性模型。使用0、10、20、40、80 μmol/L RSV 及转染PLIN2 siRNA或PKI过表达质粒后,再用40 μmol/L RSV干预24 h,油红O染色检测脂滴形成情况,酶法检测细胞内甘油三酯以及细胞外游离甘油含量,Western blot检测PLIN2蛋白表达,实时定量PCR检测PLIN2 mRNA表达。结果    RSV可以降低脂肪变性HepG2细胞脂滴蓄积程度,促进脂滴的脂解。RSV可以通过促进磷酸化增强PKA的活性,并呈浓度依赖性地上调PLIN2 mRNA和蛋白表达水平(P<0.05)。抑制PKA活性或下调PLIN2表达后,RSV促进脂肪变性HepG2细胞脂解的作用显著减弱(P<0.05)。结论     RSV通过激活PKA-PLIN2通路促进HepG2细胞脂滴脂解过程,从而可能改善非酒精性脂肪性肝病。

Abstract:

Objective     Elucidate the role of perilipinin-2 (PLIN2) in RSV improving lipid droplet metabolism in liver steatosis in HepG2 cells. Methods     HepG2 cells were incubated with 0.2 mmol/L of palmitic acid (PA) for 24 h to set up a hepatic steatosis model. The cells were then treated with or without a series of concentrations (10, 20, 40 and 80 μmol/L) of RSV, or transfected with PLIN2 siRNA or PKI overexpression plasmid, 40 μmol/L RSV was added for another 24 h. Hepatic lipid accumulation was assayed by Oil Red O staining. Contents of triglyceride in cells and free glycerin glycerol in culture medium were detected by Enzyme method. The protein and mRNA levels of PLIN2 were measured by Western blot assay and real-time quantitative PCR, respectively. Results     RSV reduced the lipid droplet accumulation and increased the lipolysis in PA-induced HepG2 cells. Additionally, RSV increased the activity of PKA by phosphorylation and dose-dependently raised the mRNA and protein levels of PLIN2(P<0.05). The promoting effect of RSV on the lipolysis was significantly reduced by the inhibition of PKA activity and the down-regulation of PLIN2 (P<0.05). Conclusion     RSV promotes lipid droplet lipolysis by activating PKA-PLIN2 pathway in HepG2 cells, and may attenuate non-alcoholic fatty liver disease.

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更新日期/Last Update: 2017-12-26