[1]陈璟,杨金锁,罗宝玉,等.Notch1调控小鼠脑出血后海马神经发生与神经功能恢复[J].第三军医大学学报,2017,39(24):2385-2389.
 Department of Neurology,Baoji Gaoxin People&rsquo,s Hospital,et al.Notch1 regulates hippocampal neurogenesis and recovery of neurological function in mice after intracerebral hemorrhage[J].J Third Mil Med Univ,2017,39(24):2385-2389.
点击复制

Notch1调控小鼠脑出血后海马神经发生与神经功能恢复(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第24期
页码:
2385-2389
栏目:
基础医学
出版日期:
2017-12-30

文章信息/Info

Title:
Notch1 regulates hippocampal neurogenesis and recovery of neurological function in mice after intracerebral hemorrhage
作者:
陈璟杨金锁罗宝玉郝涛陈静非刘娟  
宝鸡高新人民医院神经内科,第三军医大学新桥医院神经内科
Author(s):
Department of Neurology Baoji Gaoxin People’s Hospital Baoji Shaanxi Province 721004; Department of Neurology Xinqiao Hospital Third Military Medical University Chongqing 400037 China

Department of Neurology, Baoji Gaoxin People’s Hospital, Baoji, Shaanxi Province, 721004; Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
脑出血神经干细胞水平分裂Notch1
Keywords:
intracerebral hemorrhage neural stem cells horizontal division Notch1
分类号:
R338.26;R363.22;R743.34
文献标志码:
A
摘要:

目的    探讨Notch1在小鼠脑出血(intracerebral hemorrhage,ICH)后对海马神经干细胞(neural stem cells,NSCs)分裂方式影响中的作用以及对小鼠ICH后远期神经功能恢复的影响。方法     利用免疫荧光、Western blot、转轮实验和平衡木实验等方法检测小鼠脑出血后海马NSCs的分裂方式情况、Notch1蛋白表达情况和神经功能恢复情况。结果     与Sham组比较,ICH导致小鼠海马NSCs水平分裂的比例明显降低(P<0.01),同时发现小鼠海马中Notch1的表达也明显降低(P<0.05)。促进ICH小鼠海马中Notch1的表达能够有效地增加小鼠海马NSCs水平分裂的比例(P<0.01),维持NSCs的数量,同时能够有效地增加ICH后小鼠转轮实验掉落的潜伏期以及小鼠海马中DCX阳性细胞数(P<0.01),维持神经发生的持续性,另外还能够减少ICH后小鼠平衡木行走实验腿的滑落次数(P<0.01)。结论     Notch1信号可以调控脑出血后小鼠海马NSCs的分裂方式以及促进远期神经功能恢复。

Abstract:

Objective     To investigate the roles of Notch1 in division pattern of hippocampal neural stem cells (NSCs) in mice after intracerebral hemorrhage (ICH), and its effects on long-term recovery of neurological function. Methods      Immunofluorescence assay, Western blotting, and wheel rotation test and balance beam test were used to detect the division pattern of hippocampal NSCs after ICH, Notch1 protein expression and neurological function recovery, respectively. Results      Compared with the sham group, the rate of horizontal divisions pattern of hippocampal NSCs was decreased significantly in the ICH group (P<0.01). Meanwhile, the expression of Notch1 in the hippocampus was also reduced obviously (P<0.01). While, up-regulation of Notch1 through its activator treatment increased the ratio of horizontal divisions pattern of NSCs and the count of DCX-positive cells in the hippocampus of ICH mice (P<0.01), maintained the number of NSCs, and effectively prolonged the time on rotarod in wheel rotation test (P<0.01). What’s more, the treatment maintained hippocampal neurogenesis and decreased the times of foot faults in balance beam test (P<0.01). Conclusion      Notch1 signal mediates the division pattern of the hippocampal NSCs in mice after ICH and promotes long-term neurological recovery.

参考文献/References:

[1]ZHOU M, WANG H, ZHU J, et al. Causespecific mortality for 240 causes in China during 19902013: a systematic subnational analysis for the Global Burden of Disease Study 2013[J]. Lancet, 2016, 387(10015): 251-272. DOI: 10.1016/ S01406736(15)005516.
[2]MOSKOWITZ M A, LO E H, Iadecola C. The science of stroke: mechanisms in search of treatments[J]. Neuron, 2010, 67(2): 181-198. DOI: 10.1016/j.neuron.2010.07.002.
[3]LEI C, WU B, CAO T, et al. Activation of the highmobility group box 1 proteinreceptor for advanced glycation endproducts signaling pathway in rats during neurogenesis after intracerebral hemorrhage[J]. Stroke, 2014, 46(2): 500-506. DOI: 10.1161/strokeaha.114.006825.
[4]ZHAO Y, WEI Z Z, ZHANG J Y, et al. GSK3β inhibition induced neuroprotection, regeneration, and functional recovery after intracerebral hemorrhagic stroke[J]. Cell Transplant, 2017, 26(3): 395-407. DOI:10.3727/096368916X694364.
[5]NOCTOR S C, MARTNEZCERDEO V, KRIEGSTEIN A R. Distinct behaviors of neural stem and progenitor cells underlie cortical neurogenesis[J]. J Comp Neurol, 2008, 508(1): 28-44. DOI: 10.1002/cne.21669.
[6]WANG Y C, ZHOU Y, FANG H, et al. Tolllike receptor 2/4 heterodimer mediates inflammatory injury in intracerebral hemorrhage[J]. Ann Neurol, 2014, 75(6): 876-889. DOI: 10.1002/ana.24159.
[7]FENN A M, GENSEL J C, HUANG Y, et al. Immune activation promotes depression 1 month after diffuse brain injury: a role for primed microglia[J]. Biol Psychiatry, 2014, 76(7): 575-584. DOI: 10.1016/j.biopsych.2013.10.014.
[8]LIU L, ZHANG Q, CAI Y, et al. Resveratrol counteracts lipopolysaccharideinduced depressivelike behaviors via enhanced hippocampal neurogenesis[J]. Oncotarget,2016,7(35): 56045-56059. DOI: 10.18632/oncotarget.11178.
[9]SLATER J L, LANDMAN KA, HUGHES B D, et al. Cell lineage tree models of neurogenesis[J]. J Theor Biol,2009,256(2): 164-179. DOI: 10.1016/j.jtbi.2008.09.034.
[10]ARTAVANISTSAKONAS S, RAND M D, LAKE R J. Notch signaling: cell fate control and signal integration in development[J]. Science, 1999, 284(5415): 770-776.
[11]ZHANG K, ZHAO T, HUANG X, et al. Notch1 mediates postnatal neurogenesis in hippocampus enhanced by intermittent hypoxia[J]. Neurobiol Dis, 2014,64: 66-78. DOI: 10.1016/ j.nbd.2013.12.010.
[12]NTELIOPOULOS G, GORDON M Y. Protein segregation between dividing hematopoietic progenitor cells in the determination of the symmetry/asymmetry of cell division[J]. Stem Cells Dev, 2012, 21(14): 2565-2580. DOI: 10.1089/scd.2011.0467.
[13]SUN F, MAO X, XIE L, et al. Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia[J]. Aging Cell, 2013, 12(6): 978-987. DOI: 10.1111/acel.12134.
[14]WANG S, YUAN Y, XIA W, et al. Neuronal apoptosis and synaptic density in the dentate gyrus of ischemic rats’ response to chronic mild stress and the effects of Notch signaling[J]. PLoS ONE, 2012, 7(8): e42828. DOI: 10.1371/journal. pone. 0042828.
[15]KIM K, PARK H W, MOON H E, et al. The effect of human umbilical cord bloodderived mesenchymal stem cells in a collagenaseinduced intracerebral hemorrhage rat mondel[J]. Exp Neurobiol, 2015, 24(2): 146-155. DOI: 10.5607/en.2015.24.2.146.
[16]CHEN J, LEAK R K, YANG G Y. Perspective for stroke and brain injury research: mechanisms and potential therapeutic targets[J]. CNS Neurosci Ther, 2015, 21(4): 301-303. DOI: 10.1111/cns.12392.
[17] QIAO L Y, HUANG F J, ZHAO M, et al. A twoyear followup study of cotransplantation with neural stem/progenitor cells and mesenchymal stromal cells in ischemic stroke patients[J]. Cell Transplant, 2014, 23(Suppl 1): S65-S72. DOI: 10.3727/096368914X684961.

相似文献/References:

[1]陈锦华,张治元,尹昌林,等.Mash1在室管膜前下区神经干细胞向GABA能神经元分化中的作用[J].第三军医大学学报,2007,29(10):863.
 CHEN Jin-hua,ZHANG Zhi-yuan,YIN Chang-lin,et al.Role of Mash1 in differentiation of SVZa neural stem cells to GABA neurons[J].J Third Mil Med Univ,2007,29(24):863.
[2]李志方,唐军,李露斯,等.C17.2神经干细胞移植Aβ1-40损伤大鼠海马后的分化及对学习记忆的改善[J].第三军医大学学报,2008,30(07):595.
 LI Zhi-fang,TANG Jun,LI Lu-si,et al.Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats[J].J Third Mil Med Univ,2008,30(24):595.
[3]黄河清,唐军,周振华,等.西酞普兰对血管性痴呆大鼠海马DG神经发生和认知障碍影响的初步研究[J].第三军医大学学报,2008,30(10):946.
 HUANG He-qing,TANG Jun,ZHOU Zhen-hua,et al.Effects of Citalopram on hippocampal neurogenesis and cognitive functions in rats of transient cerebral ischemia[J].J Third Mil Med Univ,2008,30(24):946.
[4]储卫华,詹梦熊,朱刚,等.EDS对新生SD大鼠海马神经干细胞增殖分化的作用[J].第三军医大学学报,2008,30(19):1787.
 CHU Wei-hua,ZHAN Meng-xiong,ZHU Gang,et al.Effects of ecdysterone on proliferation and differentiation of rat neural stem cells in vitro[J].J Third Mil Med Univ,2008,30(24):1787.
[5]陈锦华,杨辉,尹昌林,等.BMP2在SVZa神经干细胞向GABA能神经元分化中的调控作用[J].第三军医大学学报,2007,29(08):699.
 CHEN Jin-hua,YANG Hui,YIN Chang-lin,et al.Role of BMP2 in differentiation of neural stem cells from anterior subventricular zone into GABAergic neurons[J].J Third Mil Med Univ,2007,29(24):699.
[6]刘学强,杨辉,何家全,等.hTERT转染神经干细胞端粒酶活性及hTERT mRNA检测[J].第三军医大学学报,2006,28(12):1292.
[7]陈兴书,姚忠祥,刘建军,等.Id2在成年大鼠喙侧神经干细胞迁移流通道的表达[J].第三军医大学学报,2005,27(10):945.
[8]宋业纯,杨辉,刘仕勇,等.高密度Oligo基因芯片筛选小鼠嗅球发育相关基因的研究[J].第三军医大学学报,2005,27(09):841.
[9]惠玲,刘建军,姚忠祥,等.DAT1基因在大鼠神经干细胞分化中作用的初步研究[J].第三军医大学学报,2005,27(07):588.
[10]黄磊,储卫华,袁继超,等.丙戊酸钠通过p21调控大鼠神经干细胞的增殖[J].第三军医大学学报,2013,35(06):487.
 Huang Lei,Chu Weihua,Yuan Jichao,et al.Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway[J].J Third Mil Med Univ,2013,35(24):487.

更新日期/Last Update: 2017-12-26