[1]水峥嵘,唐雪元.GP73通过p53信号通路调节乳腺癌细胞MCF-水峥嵘,唐雪元7的增殖和凋亡[J].第三军医大学学报,2018,40(01):64-69.
 SHUI Zhengrong,TANG Xueyuan.GP73 regulates proliferation and apoptosis of breast cancer MCF-7 via p53 signaling pathway[J].J Third Mil Med Univ,2018,40(01):64-69.
点击复制

GP73通过p53信号通路调节乳腺癌细胞MCF-水峥嵘,唐雪元7的增殖和凋亡(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第01期
页码:
64-69
栏目:
基础医学
出版日期:
2018-01-15

文章信息/Info

Title:
GP73 regulates proliferation and apoptosis of breast cancer MCF-7 via p53 signaling pathway
作者:
水峥嵘唐雪元
中南大学湘雅三医院肿瘤科
Author(s):
SHUI Zhengrong TANG Xueyuan

Department of Oncology, the Third Xiangya Hospital, Central South University, 410013, Changsha, Hunan Province, China

关键词:
Gp73p53乳腺癌细胞增殖凋亡
Keywords:
Golgi protein 73 p53 breast cancer cells proliferation apoptosis
分类号:
R394.3; R730.23; R737.9
文献标志码:
A
摘要:

目的     探讨高尔基蛋白73(GP73)对乳腺癌细胞MCF-7的增殖、凋亡的影响及相关机制。方法     利用干扰及过表达GP73质粒转染乳腺癌细胞MCF-7,并利用CCK-8检测处理后细胞的增殖能力,利用流式细胞术方法检测处理组细胞的凋亡水平,利用qRT-PCR和Western印迹法测定GP73和p53 mRNA和蛋白的表达。采用qRT-PCR测定20例临床乳腺癌和癌旁组织中GP73和p53的mRNA水平,并分析乳腺癌组织中GP73和p53的mRNA水平的相关性。结果     过表达GP73后MCF-7较对照组增殖能力上升(P<0.05),凋亡水平下降(P<0.05);干扰GP73后MCF-7增殖能力下降(P<0.05),凋亡率升高 。同时过表达GP73和p53的乳腺癌细胞较单独过表达GP73的MCF-7细胞的增殖能力下降,凋亡水平上升。乳腺癌组织中GP73和p53 mRNA表达也呈负相关(r=-0.528,P<0.01)。结论     GP73可能通过p53信号通路调节乳腺癌细胞MCF-7的增殖和凋亡。

Abstract:

Objective     To determine the impact of Golgi protein 73 (GP73) on the proliferation and apoptosis of breast cancer MCF-7 cells and investigate its related mechanism. Methods    After MCF-7 cells were separately transfected with GP73 shRNA and overexpression plasmids, CCK-8 assay and flow cytometry were employed to detect the proliferation and apoptosis, respectively in the transfected cells. The expression of GP73 and p53 at mRNA and protein levels was determined by qRT-PCR and Western blotting. The mRNA expression of above 2 molecules was detected in 20 breast cancer tissues and paired paracancerous tissues with qRT-PCR. The correlation of GP73 and p53 mRNA levels in breast cancer tissue were analyzed. Results     GP73 overexpression enhanced the proliferation and suppressed the apoptosis of MCF-7 cells, while GP73 down-regulation inhibited the proliferation and improved apoptosis of MCF-7 cells. Further, co-overexpression of GP73 and p53 resulted in reduced proliferation and increased apoptosis when compared with the MCF-7 cells overexpressed with GP7 or p53 alone. A negative correlation was observed between the mRNA expression levels of GP73 and p53 in breast cancer tissues. Conclusion     GP73 may regulate proliferation and apoptosis of breast cancer MCF-7 cells via p53 signaling pathway.

参考文献/References:

[1]KLADNEY R D, BULLA G A, GUO L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection[J]. Gene, 2000, 249(1-2): 53-65. DOI:10.1016/s0378-1119(00)00136-0.
[2]BLOCK T M, COMUNALE M A, LOWMAN M, et al. Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans[J]. Proc Natl Acad Sci USA, 2005, 102(3): 779-784. DOI:10.1073/pnas.0408928102.
[3]JIN D, TAO J, LI D, et al. Golgi protein 73 activation of MMP-13 promotes hepatocellular carcinoma cell invasion[J]. Oncotarget, 2015, 6(32): 33523-33533. DOI:10.18632/oncotarget.5590.
[4]王翠, 魏从文, 邹德勇, 等. 高尔基蛋白73参与炎症反应的分子机制研究[J]. 军事医学, 2016, 40(4): 304-307. DOI:10.7644/j.issn.1674-9960.2016.04.009.
WANG C, WEI C W, ZOU D Y, et al. Molecular mechanism of Golgi protein 73 in inflammation [J]. Mil  Med Sci, 2016, 40(4): 304-307. DOI:10.7644/j.issn.1674-9960.2016.04.009.
[5]JIANG K, LI W, SHANG S, et al. Aberrant expression of Golgi protein 73 is indicative of a poor outcome in hepatocellular carcinoma[J]. Oncol Rep, 2016, 35(4): 2141-2150. DOI:10.3892/or.2016.4601.
[6]ZHANG A, CAO B. Generation and characterization of an antiGP73 monoclonal antibody for immunoblotting and sandwich ELISA[J]. J Biomed Res, 2012, 26(6): 467-473. DOI:10.7555/JBR.26.20120057.
[7]LIANG H, BLOCK T M, WANG M, et al. Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73[J]. Cancer Biomark, 2012, 11(4): 161-171. DOI:10.3233/CBM-2012-00276.
[8]BAO Y X, CAO Q, YANG Y, et al. Expression and prognostic significance of golgiglycoprotein73 (GP73) with epithelialmesenchymal transition (EMT) related molecules in hepatocellular carcinoma (HCC)[J]. Diagn Pathol, 2013, 8: 197. DOI:10.1186/1746-1596-8-197.
[9]CHEN X, WANG Y, TAO J, et al. mTORC1 up-regulates GP73 to promote proliferation and migration of hepatocellular carcinoma cells and growth of xenograft tumors in mice[J]. Gastroenterology, 2015, 149(3): 741-752. DOI:10.1053/j.gastro.2015.05.005.
[10]邝中淑, 曾兴, 王萍, 等. SASH1基因通过p53信号通路调节乳腺癌细胞增殖[J]. 中华乳腺病杂志:电子版, 2016, 10(5):269-275. DOI:10.3877/cma.j.issn.1674-0807.2016.05.004.
KUANG Z S, ZENG X, WANG P, et al. SASH1 regulates proliferation of breast cancer cells via p53 signaling pathway [J]. Chin J Breast Dis (Electr Vers), 2016, 10(5):269-275. DOI:10.3877/cma.j.issn.1674-0807.2016.05.004.
[11]HENRY H, THOMAS A, SHEN Y, et al. Regulation of the mitochondrial checkpoint in p53-mediated apoptosis confers resistance to cell death[J]. Oncogene, 2002, 21(5): 748-760. DOI:10.1038/sj.onc.1205125.

相似文献/References:

[1]娄四龙,霍钢,郑履平,等.PTTG、bFGF和P53的表达与垂体腺瘤侵袭性的关系[J].第三军医大学学报,2007,29(18):1790.
 LOU Si-long,HUO Gang,ZHENG Lu-ping,et al.Relationship of PTTG, bFGF, P53 expressions with invasiveness of pituitary adenoma[J].J Third Mil Med Univ,2007,29(01):1790.
[2]何云燕,夏云.p53水平对端粒酶逆转录酶表达的调节作用[J].第三军医大学学报,2006,28(04):345.
[3]李开龙,何娅妮,赵玲,等.甘草酸18α体上调p53的表达保护肾脏缺血再灌注损伤的实验研究[J].第三军医大学学报,2008,30(22):2078.
 LI Kai-long,HE Ya-ni,ZHAO Ling,et al.Glycyrrhizin-18α protects against renal ischemia-reperfusion injury by upregulating p53 expression[J].J Third Mil Med Univ,2008,30(01):2078.
[4]王燕,郭乔楠,章波,等.组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控[J].第三军医大学学报,2007,29(04):300.
 WANG Yan,GUO Qiao-nan,ZHANG Bo,et al.Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6[J].J Third Mil Med Univ,2007,29(01):300.
[5]谢家印,王东,杨镇洲,等.化疗耐药相关基因在弥漫性大B细胞淋巴瘤中的表达及其临床意义[J].第三军医大学学报,2006,28(24):2478.
[6]宋华培,黄跃生,党永明,等.PI3K/Akt信号途径抑制烧伤后大鼠缺血缺氧心肌细胞凋亡[J].第三军医大学学报,2009,31(01):52.
 SONG Hua-pei,HUANG Yue-sheng,DANG Yong-ming,et al.PI3K/Akt signal pathway inhibits ischemia and hypoxia-induced myocardial apoptosis in rats[J].J Third Mil Med Univ,2009,31(01):52.
[7]刘文斌,刘晋袆,周紫垣,等.大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达[J].第三军医大学学报,2009,31(07):577.
 LIU Wen-bin,LIU Jin-yi,ZHOU Zi-yuan,et al.Dynamic expression of cell cycle regulation related proteins during carcinogenesis of lung squamous cell carcinoma in rats[J].J Third Mil Med Univ,2009,31(01):577.
[8]赵世明,杨锦建,贾占奎,等.Plk1和P53蛋白在肾上腺皮质肿瘤中的表达及临床意义[J].第三军医大学学报,2011,33(20):2209.
[9]丁雅明,方廖琼,周兰,等.不同孕期小鼠羊水对小鼠肝癌细胞H22增殖和凋亡的影响[J].第三军医大学学报,2008,30(12):1136.
 DING Ya-ming,FANG Liao-qiong,ZHOU Lan,et al.Effects of mouse amniotic fluid collected on different gestational days on proliferation and apoptosis of H22 cells in vitro[J].J Third Mil Med Univ,2008,30(01):1136.
[10]谢家印,王东,牟江洪,等.外周T细胞淋巴瘤APE1和P53蛋白联合表达的临床意义[J].第三军医大学学报,2009,31(06):510.
 XIE Jia-yin,WANG Dong,MOU Jiang-hong,et al.Coexpression and clinical significance of APE1 and P53 in peripheral T-cell lymphomas: report of 178 cases[J].J Third Mil Med Univ,2009,31(01):510.

更新日期/Last Update: 2018-01-12