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 QIN Wanxiang,LUO Min,SHI Ying,et al.Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells[J].J Third Mil Med Univ,2017,39(22):2189-2194.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第22期
页码:
2189-2194
栏目:
基础医学
出版日期:
2017-11-30

文章信息/Info

Title:
Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells
作者:
覃万翔罗敏石英崔剑聂发传骆世芳贺桂琼汪克建
重庆医科大学神经科学研究中心;第三军医大学西南医院疼痛科
Author(s):
QIN Wanxiang LUO Min SHI Ying CUI Jian NIE Fachuan LUO Shifang HE Guiqiong WANG Kejian

Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016; Department of Pain Management, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

关键词:
双氢青蒿素脂多糖BV2小胶质细胞炎症因子核转录因子&kappaB
Keywords:
dihydroartemisinin lipopolysaccharide BV-2 microglial cells inflammatory cytokines NF-&kappaB pathway
分类号:
R282.71;R322.8;R364.5
文献标志码:
A
Abstract:

Objective      To investigate the inhibitory effect of dihydroartemisinin (DHA) on lipopolysaccharide (LPS)induced inflammatory response in BV-2 microglial cells. Methods     Inflammatory model was established by exposure of BV-2 cells to LPS for 24 h. Then after the cells were treated with different concentrations of DHA (0.5, 1, 2 and 4 μmol/L), cell viability was tested with CCK-8 assay. The cell morphology was observed under inverted phase contrast microscope after the inflammatory cell model under the treatment of a harmless concentration (1 μmol/L) of DHA, The mRNA expression of iNOS, IL-1β, IL-6 and TNF-α was measured by RT-PCR. The contents of TNFα, IL-1β and IL-6 in the supernatant were detected by ELISA. The expression of the nuclear NFκB (p65) , IκBα, and TLR4 were analyzed by Western blotting. Results     DHA showed no significant effect on cell viability when the concentration was lower than 2 μmol/L (P>0.05). DHA inhibited the cell morphological changes induced by LPS. DHA treatment resulted in the decreases in the mRNA expression levels of iNOS, IL-1β, IL-6 and TNF-α (P<0.01), the production of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, the protein levels of TLR4, intracytoplasmic IκBα and the nuclear translocation of NF-κB p65 (P<0.01). Conclusion     DHA inhibits the inflammatory responses, activation of NF-κB of BV-2 microglial cells induced by LPS, and thus suppresses the production of pro-inflammatory cytokines, via TLR4/NF-κB signaling pathway.

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更新日期/Last Update: 2017-11-28