[1]高建芳,杨桓,邹鹏,等.成年男性精子线粒体DNA拷贝数及完整性与精液参数的关联研究[J].第三军医大学学报,2017,39(12):1286-1291.
 Gao Jianfang,Yang Huan,Zou Peng,et al.Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults[J].J Third Mil Med Univ,2017,39(12):1286-1291.
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成年男性精子线粒体DNA拷贝数及完整性与精液参数的关联研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第12期
页码:
1286-1291
栏目:
公共卫生与预防医学
出版日期:
2017-06-30

文章信息/Info

Title:
Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults
作者:
高建芳杨桓邹鹏陈卿张国伟凌曦陈宏强敖琳曹佳
宁夏医科大学公共卫生与管理学院;第三军医大学军事预防医学院毒理学研究所
Author(s):
Gao Jianfang Yang Huan Zou Peng Chen Qing Zhang Guowei Ling Xi Chen Hongqiang Ao Lin Cao Jia

School of Public Health and Management, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004; Institute of Toxicology, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
精液线粒体DNA相关性分析
Keywords:
sperm quality mitochondrial DNA association analysis
分类号:
R321.1; R339.2; R394.2
文献标志码:
A
摘要:

目的        分析成年男性精子线粒体DNA(mitochondrial DNA,mtDNA)拷贝数及完整性与常规精液质量参数的关联,探讨精子mtDNA相关指标在反映男性生育力中的作用。方法        采集161例参加男性生殖健康调查的志愿者的精液样本。采用计算机辅助精子分析系统(computer-aided sperm analysis,CASA)进行精液参数分析,Diff-Quick试剂盒染色检测精子形态。实时荧光定量PCR测定精子mtDNA拷贝数,长链PCR检测mtDNA完整性。结果         mtDNA拷贝数与常规精液参数包括精液体积、精子密度、精子总数、前向运动率、精子活率和精子正常形态率均呈负相关 (r=-0.168、-0.452、-0.474、-0.189、-0.306、-0.235,P均<0.05)。除精子前向运动率外,mtDNA完整性与其余常规精液参数均呈正相关 (r=0.193、0.379、0.480、0.325、0.282,P均<0.05)。采用多元线性回归分析调整混杂因素后,mtDNA相关指标与精子密度、精子总数、精子活率和正常形态率之间的相关性仍然存在。结论        mtDNA拷贝数和mtDNA完整性与常规精液质量参数之间存在关联,mtDNA相关指标可能是反映精子质量的生物标志物。

Abstract:

Objective      To analyze the association of sperm mitochondrial DNA (mtDNA) copy number and mtDNA integrity with semen quality parameters and investigate the role of the sperm mtDNA-related indicators in assessing fertility in male adults. Methods       We collected semen samples from 161 male adult volunteers in a male reproductive health survey. The routine semen parameters were examined using a computer-aided sperm analysis system (CASA), and Diff-Quick kit was used to evaluate the sperm morphology. Quantitative long polymerase chain reaction (L-PCR) and realtime fluorescence quantitative PCR (Q-PCR) were used to assess the mtDNA integrity and detect mtDNA copy number in the spermatozoa. Results      We found inverse correlations of sperm mtDNA copy number with the semen volume, sperm concentration, total sperm count, progressive motile sperm count, total motility and the rate of normal sperm morphology (r=-0.168,-0.452,-0.474,-0.189,-0.306,-0.235,all P<0.05). Sperm mtDNA integrity was positively correlated with the semen volume, sperm concentration, total sperm count, total motility and the rate of normal sperm morphology (r=0.193,0.379,0.480,0.325,0.282,all P<0.05) except for progressive motile sperm count. Multiple linear regression analysis showed significant correlations of mtDNA copy number and mtDNA integrity with the sperm concentration, total sperm count, sperm motility, and the rate of normal sperm morphology after adjustment for the confounders. Conclusion       Sperm mtDNA copy number and mtDNA integrity are significantly correlated with the routine semen quality parameters, suggesting that these two mtDNA-related indicators may serve as potential biomarkers for assessing sperm quality in male adults.

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更新日期/Last Update: 2017-06-27