[1]周衍,范俊,蒋叶华,等.时间分辨荧光微球免疫层析技术定量检测人血清胃蛋白酶原Ⅰ的实验研究 [J].第三军医大学学报,2017,39(18):1803-1807.
 ZHOU Yan,FAN Jun,JIANG Yehua,et al.Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography[J].J Third Mil Med Univ,2017,39(18):1803-1807.
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时间分辨荧光微球免疫层析技术定量检测人血清胃蛋白酶原Ⅰ的实验研究
 
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第18期
页码:
1803-1807
栏目:
基础医学
出版日期:
2017-09-30

文章信息/Info

Title:
Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography
作者:
周衍范俊蒋叶华张珏张艺傅利新黄飚
江苏省原子医学研究所;无锡市江原实业技贸总公司
Author(s):
ZHOU Yan FAN Jun JIANG Yehua ZHANG Jue ZHANG Yi FU Lixin HUANG Biao

Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu Province, 214063; Wuxi Jiangyuan Industrial Technology and Trade Corporation, Wuxi, Jiangsu Province, 214063, China

关键词:
胃蛋白酶原Ⅰ时间分辨荧光纳米微球免疫层析
Keywords:
pepsinogen I time-resolved fluorescence nanoparticles immunochromatography
分类号:
R345.69;R446.112;R446.6
文献标志码:
A
摘要:

目的     研制快速定量检测人血清胃蛋白酶原Ⅰ(pepsinogensⅠ,PGⅠ)试纸,并评价试纸性能。方法     以包裹有时间分辨荧光的纳米微球作为标记示踪物,运用免疫层析试纸技术,研制PGⅠ检测试纸;考察试纸的检测线性、灵敏度、精密度、与对比试剂的符合率。结果    试纸定量检测的线性良好,方程为Y=0.027 9+0.004 8X,r=0.997 8;灵敏度为0.27 μg/L;批内CV小于10%,批间CV 11.03% ;与市售PGⅠ试剂平行检测489份血清样本,有良好的相关性(YTRFIA=0.856 6Xstrip+7.545 6,R2=0.989 1,P<0.01)。结论     PGⅠ时间分辨荧光纳米微球免疫层析试纸操作简单、快速、经济、可定量,满足临床检测血清PGⅠ的需求。

Abstract:

Objective      To develop a fast quantitative detection strip of pepsinogen Ⅰ (PGⅠ). Methods      With the time-resolved fluorescent nanoparticles as the marker tracer, PGⅠ strip based on doubleantibody sandwich immunochromatography was developed. The obtained strip was evaluated for its linearity, sensitivity, accuracy, and consistency with commercially available products (489 serum samples). Results      The strip showed good linearity, and the linear equation was Y=0.027 9+0.004 8X, r=0.997 8. The sensitivity of the PGⅠ strip was 0.27 μg/L. The coefficient of variation (CV) values of intra-batch was less than 10%, and that of inter-batch was 11.03%. Compared with the commercially available product (timeresolved fluorescence immunoassay) , our PGⅠ strip showed the high consistency (YTRFIA=0.856 6Xstrip+7.545 6,R2=0.989 1, P<0.01). Conclusion     Our developed time-resolved fluorescent nanoparticle immunochromatographic strip for PGⅠ detection is rapid, low cost, easy to operate, and quantitative, and can meet the needs of the clinical practice.

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更新日期/Last Update: 2017-09-29