[1]罗文英,刘建军,丁 瑜,等.黏液型与非黏液型铜绿假单胞菌的比较蛋白组学分析[J].第三军医大学学报,2017,39(13):1345-1351.
 LUO Wenying,LIU Jianjun,DING Yu,et al.Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains[J].J Third Mil Med Univ,2017,39(13):1345-1351.
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黏液型与非黏液型铜绿假单胞菌的比较蛋白组学分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第13期
页码:
1345-1351
栏目:
基础医学
出版日期:
2017-07-15

文章信息/Info

Title:
Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains
作者:
罗文英刘建军丁  瑜杨拉维任晓虎
广东医科大学附属医院检验科;深圳市疾病预防控制中心毒理实验室;广东医科大学附属医院临床研究中心
Author(s):
LUO Wenying LIU Jianjun DING Yu YANG Lawei REN Xiaohu

Department of Clinical Laboratory, Center for Clinical Research, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong Province, 524001; Laboratory of Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong Province, 518055, China

关键词:
黏液型铜绿假单胞菌生物标志物蛋白质组学
Keywords:
mucoid Pseudomonas aeruginosa biomarkers iTRAQ
分类号:
R372; R378.991; R394.33
文献标志码:
A
摘要:

目的         探讨黏液型与非黏液型铜绿假单胞菌(Pseudomonas aeruginosa,P.a)的差异蛋白谱,以发现黏液型P.a潜在的生物标志物。方法       采用来自于临床囊性纤维化患者分离的3株黏液型P.a菌株,脉冲场凝胶电泳以确定它们的同源性;采用同位素相对和绝对定量标记 (isobaric tags for relative and absolute quantitation,iTRAQ)结合二维液相色谱串联质谱(twodimensional liquid chromatographytandem mass spectrometry,2D LC-MS/MS)技术分析3株黏液型与对照组1株非黏液型P.a的差异蛋白谱,并采用RTqPCR方法对5个共同差异蛋白进行验证。结果         3株黏液型P.a菌株不具有同源性,iTRAQ结果没有明显差异。3组共有219个上调和101个下调差异蛋白。pa2815、pa2018、dnak、pa5046和acef基因转录水平与 iTRAQ结果一致。结论       不同黏液表型P.a的iTRAQ结果没有明显差异,219个上调和101个下调差异蛋白可以作为研究黏液型与非黏液型P.a致病机制的候选生物标志物。

Abstract:

Objective        To investigate the differences in protein profiling between mucoid and non-mucoid Pseudomonas aeruginosa (P.a), in order to find the potential biological markers of mucoid P.a. Methods         Three strains of mucoid P.a were isolated from the sputum of cystic fibrosis patients, and then pulsed field gel electrophoresis was used to determine their homology. Isobaric tags for relative and absolute quantitation (iTRAQ) labeling strategy combined with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) was used to investigate the differential protein abundances between the 3 mucous and 1 non-mucoid P.a strains. Differential expression of 5 common differential genes was verified by using RT-qPCR. ResultsThere was no homology in the 3 mucoid P.a strains. No significant differences were found in the iTRAQ results of them. There were 219 up-regulated and 101 down-regulated proteins identified among the 3 strains. The transcriptional levels of pa2815, pa2018, dnak, pa5046 and acef genes were consistent with the results of iTRAQ. Conclusion       There are no significant differences in the iTRAQ results of different mucous P.a strains. The obtained 219 up-regulated and 101 down-regulated differentially expressed proteins can be used as candidate biomarkers for the study of the pathogenesis of mucoid and non-mucoid P.a.

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更新日期/Last Update: 2017-07-13