[1]唐佳佳,叶林.miRNA-671上调抑制胃癌细胞增殖与迁移[J].第三军医大学学报,2017,39(16):1643-1647.
 TANG Jiajia,YE Lin.Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells[J].J Third Mil Med Univ,2017,39(16):1643-1647.
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miRNA-671上调抑制胃癌细胞增殖与迁移(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第16期
页码:
1643-1647
栏目:
基础医学
出版日期:
2017-08-30

文章信息/Info

Title:
Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells
作者:
唐佳佳叶林
重庆医科大学附属第一医院重症医学科
Author(s):
TANG Jiajia YE Lin

Department of Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
miR-671胃癌增殖迁移
Keywords:
miR-671 gastric cancer proliferation migration
分类号:
R394.3;R730.23;R735.2
文献标志码:
A
摘要:

目的     探索miR-671在胃癌组织和细胞中的表达,并研究其对胃癌细胞增殖和迁移的影响。方法    通过qRT-PCR实验检测miR671在胃癌组织和细胞中的表达,通过CCK-8实验检测miR-671表达上调对MKN45胃癌细胞增殖,通过划痕和Transwell小室实验检测miR-671表达上调对MKN45胃癌细胞侵袭和迁移的影响。结果    与癌旁正常组织相比,胃癌组织中的miR-671表达明显下调(P<0.01);与正常人胃黏膜上皮细胞相比,胃癌细胞中的miR-671表达也明显下调(P<0.01); miR-671表达上调明显抑制MKN45胃癌细胞增殖、迁移和侵袭(P<0.01)。结论     miR-671可以抑制胃癌细胞的增殖、迁移和侵袭,在胃癌中发挥抑癌基因功能。

Abstract:

Objective      To investigate the expression of miR-671 in gastric cancer tissues and cell lines, and determine its effects on the proliferation and invasion of gastric cancer cells. Methods     The expression of miR-671 was detected in gastric cancer tissues and para-carcerous tissues from 8 patients and cell lines SGC7901, MKN28 and MKN45, and human gastric mucosal epithelial cell line GES-1 by quantitative real-time PCR (qRT-PCR). CCK-8 assay was used to measure the cell proliferation after miR-671 up-regulation. The effect of miR-671 on cell migration and invasion was detected by wound healing assay and Transwell chamber assay. Results     The expression level of miR-671 was significantly decreased in gastric cancer tissues than the adjacent normal tissues (P<0.01). The expression levels of miR671 were significantly decreased in the gastric cancer cell lines compared with GES-1 cells (P<0.01). Up-regulation of miR-671 significantly inhibited the proliferation, migration and invasion in gastric cancer cells (P<0.01). Conclusion     miR-671 inhibits proliferation, migration and invasion of gastric cancer cells, and may serve as a tumor suppressor.

参考文献/References:

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更新日期/Last Update: 2017-08-23