[1]唐佳佳,叶林.下调STYK1基因表达可抑制结直肠癌细胞生长与侵袭[J].第三军医大学学报,2017,39(17):1734-1738.
 TANG Jiajia,YE Lin.STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro[J].J Third Mil Med Univ,2017,39(17):1734-1738.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第17期
页码:
1734-1738
栏目:
基础医学
出版日期:
2017-09-15

文章信息/Info

Title:
STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro
作者:
唐佳佳叶林
重庆医科大学附属第一医院重症医学科
Author(s):
TANG Jiajia YE Lin

Department of Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
miR-671直肠癌增殖迁移
Keywords:
serine/threonine/tyrosine kinase 1 colorectal cancer proliferation invasion
分类号:
R394.3; R730.23; R735.3
文献标志码:
A
摘要:

目的     探讨STYK1基因下调对结直肠癌细胞HCT-15增殖、凋亡和迁移以及MEK/ERK和PI3K/AKT信号通路的影响。方法     采用基因干扰技术抑制结直肠癌细胞HCT-15中STYK1的表达,Western blot检测抑制效果,CCK-8检测STYK1表达下调对HC-T15细胞增殖的影响,流式细胞仪检测STYK1表达下调对HCT15细胞凋亡的影响,Transwell小室实验检测STYK1表达下调对HCT-15细胞侵袭的影响,通过Western blot检测STYK1表达下调对HCT15细胞MEK/ERK和PI3K/AKT信号通路活性的影响。结果      与干扰对照组相比,STYK1表达下调明显抑制HCT-15细胞增殖活性(P<0.01)和侵袭能力(P<0.01),并诱导细胞凋亡(P<0.01);同时,STYK1表达下调明显抑制HCT-15细胞ERK1/2和AKT蛋白的磷酸水平。结论     STYK1基因干扰可以抑制结直肠癌细胞的增殖和侵袭,并诱导凋亡,其机制可能与抑制MEK/ERK和PI3K/AKT信号通路活性有关。

Abstract:

Objective     To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation, apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15. Methods     The expression of STYK1 protein was down-regulated by RNA interference technique in HCT-15 cells. Then, the STYK1 protein expression was detected by Western blotting. CCK-8 assay was used to measure the effect of STYK1 downregulation on the proliferation of the cells, flow cytometry was employed to detect the cell apoptosis, and Transwell chamber assay was adopted for cell invasion ability. The expression levels of pERK1/2 and p-AKT were detected by Western blotting. Results   Compared with the siRNA NC group, STYK1 down-regulation significantly inhibited the proliferation (P<0.01) and invasion (P<0.01), induced cell apoptosis (P<0.01), and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (P<0.01). Conclusion     STYK1 downregulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells, which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.

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更新日期/Last Update: 2017-09-04