[1]顾业芸,周启程,公欣华,等.二氢山奈酚衍生物调控AMPK/PGC-1α通路抑制棕榈酸诱导的C2C12成肌分化细胞脂质沉积[J].第三军医大学学报,2017,39(16):1606-1611.
 GU Yeyun,ZHOU Qicheng,GONG Xinhua,et al.Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway[J].J Third Mil Med Univ,2017,39(16):1606-1611.
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二氢山奈酚衍生物调控AMPK/PGC-1α通路抑制棕榈酸诱导的C2C12成肌分化细胞脂质沉积(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第16期
页码:
1606-1611
栏目:
基础医学
出版日期:
2017-08-30

文章信息/Info

Title:
Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway
作者:
顾业芸周启程公欣华朱俊东糜漫天
第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室,重庆市医学营养研究中心 
Author(s):
GU Yeyun ZHOU Qicheng GONG Xinhua ZHU Jundong MI Mantian

Department of Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
二氢山奈酚骨骼肌脂质沉积磷酸腺苷活化蛋白激酶过氧化物酶体增殖活化受体&gamma共激活因子-1&alpha
Keywords:
dihydrokaempferol skeletal muscle: lipid deposition AMP-activated protein kinase peroxisome proliferator activator receptor gamma coactivator-1alpha
分类号:
R151.3; R329.26; R587
文献标志码:
A
摘要:

目的      探讨4种二氢山奈酚衍生物对C2C12成肌分化细胞(C2C12-MD细胞)脂质沉积的影响及相关分子机制。方法      小鼠C2C12成肌细胞分化为肌管后,分成正常对照组、棕榈酸(palmitic acid,PA)处理组,PA+二氢山奈酚衍生物(山奈酚、二氢杨梅素、杨梅素、槲皮素)干预处理组(n=3),采用油红O染色和甘油三酯(triglyceride, TG)测定法评价脂质沉积情况,2-NBDG荧光法检测葡萄糖摄取能力,Western blot检测AMPK、p-AMPK和过氧化物酶体增殖活化受体γ共激活因子-1α(peroxisome proliferator activator receptor gamma coactivator-1alpha,PGC-1α)蛋白水平。结果      PA处理的C2C12肌管有大量脂质沉积,TG含量明显增加,胰岛素刺激下的葡萄糖摄取能力显著下降,p-AMPK、PGC1α蛋白水平明显降低(P<0.05)。山奈酚干预对PA诱导的上述指标变化无明显影响,但另外3种衍生物干预均能显著抑制PA诱导的上述指标变化,其中二氢杨梅素效果更明显(P<0.05)。此外,AMPK抑制剂(compound C,CC)可明显逆转二氢杨梅素的抑制效应(P<0.05)。 结论      3种二氢山奈酚衍生物二氢杨梅素、杨梅素和槲皮素可通过调控AMPK/PGC-1α通路抑制棕榈酸诱导的C2C12-MD细胞脂质沉积,继而改善胰岛素抵抗。

Abstract:

Objective     To determine the effects of 4 derivatives from dihydrokaempferol on lipid deposition in C2C12 myotubes (C2C12-MD cells) and investigate the underlying mechanisms. Methods      Mouse C2C12 myoblasts were differentiated into myotubes and then treated by palmitic acid (PA) alone or in combination with 4 derivatives of dihydrokaempferol (kaempferol, dihydromyricetin, myricetin and quercetin) respectively. Lipid deposition was evaluated by oil red O staining and triglyceride (TG) content measurement. Glucose uptake was measured by using 2-NBDG fluorescent probe. The protein levels of AMP-activated protein kinase (AMPK), phosphorylated AMPK (p-AMPK) and peroxisome proliferator activator receptor gamma coactivator-1alpha (PGC-1α) were detected by Western blotting. Results    PA induced more lipid deposition, significantly increased TG content, and significantly decreased glucose uptake under insulin stimulation as well as the protein levels of pAMPK and PGC-1α in C2C12 myotubes (P<0.05). Kaempferol treatment had no significant effects on these changes induced by PA. However, the other 3 derivatives markedly inhibited these changes induced by PA, and dihydromyricetin showed a stronger inhibitory effect (P<0.05). Moreover, AMPK inhibitor compound C could significantly reverse the inhibitory effect of dihydromyricetin (P<0.05). Conclusion      Three derivatives of dihydrokaempferol (dihydromyricetin, myricetin and quercetin) inhibit PA-induced lipid deposition in C2C12-MD cells through AMPK/PGC-1α signaling pathway, and thereby improve insulin resistance.

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更新日期/Last Update: 2017-08-18