[1]魏莱,王喆,梁后杰.6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义[J].第三军医大学学报,2017,39(10):983-989.
 Wei Lai,Wang Zhe,Liang Houjie.Expression of 6-phosphofructokinase-2 in colorectal cancer and its clinical significance[J].J Third Mil Med Univ,2017,39(10):983-989.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第10期
页码:
983-989
栏目:
基础医学
出版日期:
2017-05-30

文章信息/Info

Title:
Expression of 6-phosphofructokinase-2 in colorectal cancer and its clinical significance
作者:
魏莱王喆梁后杰
第三军医大学西南医院肿瘤科
Author(s):
Wei Lai Wang Zhe Liang Houjie

Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

关键词:
结直肠癌组织芯片6-磷酸果糖-2-激酶/果糖-26-二磷酸酶缺氧诱导因子-1
Keywords:
colorectal cancer tissue microarray 6-phosphofructokinase-2 HIF-1&alpha
分类号:
R345.57; R730.23; R735.35
文献标志码:
A
摘要:

目的检测6-磷酸果糖-2-激酶(6-phosphofructokinase-2,PFKFB)在结直肠癌及癌旁组织中的表达,并探讨其临床意义。方法应用组织芯片结合免疫组化技术检测本院90例结直肠癌组织标本和相应90例癌旁组织中PFKFB1、PFKFB2、PFKFB3、PFKFB4及缺氧诱导因子-1(HIF1-α)的表达。结果PFKFB1、PFKFB2、PFKFB3、PFKFB4和HIF1-α在90例结直肠癌组织中高表达例数分别为57、59、59、51和60,表达均显著高于相应癌旁组织(P<0.05)。PFKFB3在结直肠癌组织中的高表达与肿瘤的大小、临床分期、淋巴结转移及预后等密切相关(P<0.05),高表达组比低表达组预后差(P<0.01),临床分期高的结直肠癌患者中,PFKFB3的高表达提示了临床预后差(P<0-01),但在临床分期低的患者中不存在这种差异(P>0.05)。PFKFB3的表达同HIF-1α的表达呈正相关(r=0.627,P<0.01)。而PFKFB1、PFKFB2和PFKFB4的表达与结直肠癌的肿瘤大小、临床分期、淋巴结转移及预后等无显著相关性(P>0.05)。结论PFKFB家族在结直肠癌中高表达,且结直肠癌患者中PFKFB3高表达者比低表达者预后差,并且其作用同HIF1-α的表达密切相关。

Abstract:

ObjectiveTo investigate the expression level of 6-phosphofructokinase-2 (PFKFB) in human colorectal cancer and explore its clinical significance.  MethodsTissue microarray combined with immunohistochemistry was used to detect the expression levels of PFKFB1, PFKFB2, PFKFB3, PFKFB4 and hypoxia-inducible factor-1(HIF1-α) in 90 colorectal cancer tissue samples and 90 paired adjacent tissue samples.  ResultsHigh expression levels of PFKFB1, PFKFB2, PFKFB3, PFKFB4 and HIF1-α were detected in 57, 59, 59, 51 and 60 of the colorectal cancer tissues, respectively, and their expression levels were significantly higher than in the paired adjacent tissues (P<0.05). The expression level of PFKFB3 in the colorectal cancer tissue was closely correlated with the tumor size, clinical stage, lymph node metastasis and prognosis (P<0.05). The patients with a high PFKFB3 expression level had poorer prognosis than those with a low PFKFB3 expression (P<0.01). A high expression of PFKFB3 was associated with a poor prognosis in the patients in an advanced stage (P<0.01) but not in those in an early stage (P>0.05). The expression level of PFKFB3 was positively correlated with HIF-1α (r=0.627, P<0.01). The expression levels of PFKFB1, PFKFB2 and PFKFB4 showed no obvious correlations with the tumor size, clinical stage, lymph node metastasis, or prognosis of the patients (P>0.05).  ConclusionThe PFKFB family is highly expressed in colorectal cancers. A high expression of PFKFB3, which is positively correlated with HIF-1α expression, is associated with a poor prognosis of the patients, suggesting the potential role of PFKFB3 in tumorigenesis and progression of colorectal cancer.

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更新日期/Last Update: 2017-05-22