[1]程和平,曾梁楠,黄智蒙,等.980 nm连续激光在金属医疗器械表面高效灭菌的实验研究[J].第三军医大学学报,2017,39(09):878-883.
 Cheng Heping,Zeng Liangnan,Huang Zhimeng,et al.Sterilizing effect of 980 nm continuous laser on metal surface of medical equipment[J].J Third Mil Med Univ,2017,39(09):878-883.
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980 nm连续激光在金属医疗器械表面高效灭菌的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第09期
页码:
878-883
栏目:
基础医学
出版日期:
2017-05-15

文章信息/Info

Title:
Sterilizing effect of 980 nm continuous laser on metal surface of medical equipment
作者:
程和平曾梁楠黄智蒙陈琰琰陈图南冯华鲜继淑
黄山学院信息工程学院;工程物理研究院流体物理研究所;第三军医大学西南医院神经外科;西南医科大学第一附属医院神经外科
Author(s):
Cheng Heping Zeng Liangnan Huang Zhimeng Chen Yanyan Chen Tunan Feng Hua Xian Jishu

School of Information Engineering, Huangshan University, Huangshan, Anhui Province, 245041; Institute of Fluid Physics, Chinese Academy of Engineering Physics, Mianyang, Sichuan Province, 621900; Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Department of Neurosurgery, the First Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China

关键词:
红外激光灭菌反应动力学方程金属医疗器械
Keywords:
infrared laser sterilization kinetics equation metal medical equipment
分类号:
R187.4;R197.39
文献标志码:
A
摘要:

目的      探讨980 nm红外连续激光对金属医疗器械表面高效灭菌的功率和时间阈值关系。方法      采用人工污染实验法,以无菌钢制手术刀为载体,用金黄色葡萄球菌和大肠杆菌分别进行污染,经不同功率、对应不同辐照时间的激光消毒灭菌后用细菌培养基清洗刀片采样,采用增菌培养来检测红外激光对大肠杆菌和金黄色葡萄球菌的灭活情况。结果      980 nm激光在5.24 W时辐照29 s,6.87 W时辐照24 s,8.33 W时辐照14 s,10.11 W时辐照14 s都能彻底杀死金黄色葡萄球菌;980 nm激光在5.24 W时辐照22 s,6.87 W时辐照12 s,8.33 W时辐照11 s,10.11 W时辐照9 s都能彻底杀死大肠杆菌。大肠杆菌比金黄色葡萄球菌更易灭活。依据实验数据拟合,在同一激光功率下,两种细菌存活率与辐照时间的函数符合一阶反应动力学形式。结论      980 nm红外激光能够实现金属医疗器械表面的快速消毒灭菌,并且功率越高则时间越短、效果越好。

Abstract:

Objective      To investigate the relationship of power and exposure period of 980 nm continuous laser system on the surface sterilization for metal medical equipment. Methods      The sterile steel lancets were artificially contaminated with Staphylococcus aureus and Escherichia coli, respectively. These lancets were exposed and disinfected by 980 nm continuous laser at different powers for different time periods. The inactivation of the bacteria on each lancet was observed and measured by 8hour enrichment culture. Results      Staphylococcus aureus were thoroughly killed after the lancets were disinfected by the 980 nm laser at 5.24 W for 29 s, 6.87 W for 24 s, 8.33 W for 14 s, and 10.11 W for 14 s, respectively. The power and exposure period for Escherichia coli were 5.24 W for 22 s, 6.87 W for 12 s, 8.33 W for 11 s, and 10.11 W for 9 s, respectively. Escherichia coli was more likely to be inactivated than Staphylococcus aureus. According to the experimental data, the survival rate under same power of infrared laser as a function of irradiation time was fitted to the first-order kinetics equation. Conclusion     980 nm infrared laser can achieve rapid sterilization on metal surface of medical equipment in a very short time period. The larger power the laser is with, the shorter is the exposure period, and the better the sterilizing effect is.

参考文献/References:

[1]中华人民共和国卫生部. 医疗机构消毒技术规范:GB/T1.1-2009[S]. 北京: 中国标准出版社, 2012: 25-41.
The Ministry of Health of the People’s Republic of China. Regulation of disinfection technique in healthcare settings: GB/T1.1-2009[S]. Beijing: Standards Press of China, 2012: 25-41.
[2]黄秋华, 涂慧慧, 吴莉. 三种消毒液对硅胶气管套管消毒效果比较[J]. 中国消毒学杂志, 2012, 29(3): 192-193.
Huang Q H, Tu H H, Wu L. Comparison of disinfection efficacy of three liquid disinfectants on silicone tracheal cannula[J]. Chinese Journal of Disinfection, 2012, 29(3): 192-193.
[3]李钟响, 吴红映, 金美琴. 常见细菌对紫外线敏感性试验观察[J]. 中华医院感染学杂志, 2012, 22(1): 121-122.
Li Z X, Wu H Y, Jin M Q. Experimental observation of ultraviolet light sensitivity effect on common bacteria[J]. Chinese Journal of Nosocomiology, 2012, 22(1): 121-122.
[4]周逞丽. 快速生物监测法对高压蒸汽灭菌效果监测的评价[J]. 实用预防医学, 2008, 15(3): 885-886. DOI:10.3969/j.issn.1006-3110.2008.03.120.
Zhou C L. Effect Evaluation of a Rapid Biological Monitoring Method in High—Pressure Sterilization[J]. Practical Preventive Medicine, 2008, 15(3): 885-886. DOI:10.3969/j.issn.10063110.2008.03.120.
[5]Deppe H, Horch H H, Schr-dl V, et al. Effect of 308nm excimer laser light on peri-implantitisassociated bacteria: an in vitro investigation[J]. Lasers Med Sci, 2007, 22(4): 223-227. DOI:10.1007/s10103-007-0441-2.
[6]Mohaidat Q I. Laser-induced breakdown spectroscopy (LIBS): an innovative tool for studying bacteria[D]. Detroit:Graduate Schoolof Wayne State University, 2011.
[7]Ando Y, Aoki A, Watanabe H, et al. Bactericidal effect of erbium YAG laser on periodontopathic bacteria[J]. Lasers Surg Med, 1996, 19(2): 190-200. DOI:10.1002/(SICI)1096-9101(1996) 19:2<190::aid-lsm11>3.0.CO;2B.
[8]李若兰, 任煜光, 孙风阳. 脉冲Nd:YAG激光对几种细菌杀菌作用的实验研究[J]. 口腔医学, 2000, 20(1): 16-17. DOI:10.3969/j.issn.1003-9872.2000.01.008.
Li R L, Ren Y G, Sun F Y. Experimental study on bactericidal effects of pulsed nd:YAg laser[J]. Stomatology, 2000, 20(1): 16-17. DOI:10.3969/j.issn.1003-9872.2000.01.008.
[9]胡鹏, 陈发良. 重复频脉冲激光辐照金属材料热效应模拟分析[J]. 中国激光, 2016, 43(10): 1001006. DOI: 10.3788/cjl201643.1001006.
Hu P, Chen F L. Simulation of thermal effects in repetitive pulsed laser irradiation on metals[J]. Chinese Journal of Lasers, 2016, 43(10): 1001006. DOI: 10.3788/cjl201643.1001006.
[10]Ongeng D, Haberbeck L U, Mauriello G, et al. Modeling the Fate of Escherichia coli O157:H7 and Salmonella enterica in the Agricultural Environment: Current Perspective[J]. J Food Sci, 2014, 79(4): R421-R427. DOI:10.1111/1750-3841.12392.
[11]Hiatt C W. Kinetics of the inactivation of viruses [J]. Bacteriol Rev, 1964, 28 (2): 150-163.
[12]杭锋, 陈卫, 陈帅, 等. 微波加热对大肠杆菌与金黄色葡萄球菌蛋白质与核酸变性的影响[J].食品与机械, 2008, 24(5): 12-15.
Hang F, Chen W, Chen S, et al. The effect of microwave heating on the denaturation of E.coli and S.aureus intracelluar protein and nucleic acid[J]. Food and Machinery, 2008, 24(5):12-15.
[13]Woo I S, Rhee I K, Park H D. Differential Damage in Bacterial Cells by Microwave Radiation on the Basis of Cell Wall Structure[J]. Appl Environ Microbiol, 2000, 66(5): 2243-2247. DOI:10.1128/aem.66.5.2243-2247.2000.

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更新日期/Last Update: 2017-05-05