[1]陈侯磬,汪洋,刘扬,等.橄榄苦苷对破骨细胞的分化以及骨吸收功能的影响[J].第三军医大学学报,2017,39(14):1435-1439.
 CHEN Houqing,WANG Yang,LIU Yang,et al.Effect of oleuropein on osteoclast differentiation and bone resorption in vitro[J].J Third Mil Med Univ,2017,39(14):1435-1439.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第14期
页码:
1435-1439
栏目:
基础医学
出版日期:
2017-07-30

文章信息/Info

Title:
Effect of oleuropein on osteoclast differentiation and bone resorption in vitro
作者:
陈侯磬汪洋刘扬田冬冬袁威张然熙邓忠良
重庆医科大学附属第二医院骨科
Author(s):
CHEN Houqing WANG Yang LIU Yang TIAN Dongdong YUAN Wei ZHANG Ranxi DENG Zhongliang  

Department of Orthopaedics, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China

关键词:
橄榄苦苷RAW264.7细胞破骨细胞分化骨吸收
Keywords:
oleuropein RAW264.7 cells osteoclast differentiation bone resorption
分类号:
R282.71;R322.71;R329.28
文献标志码:
A
Abstract:

ObjectiveTo investigate the effect of oleuropein on the differentiation and bone resorption of cultured osteoclasts and explore the possible mechanism. MethodsBone marrow macrophages from BALB/c mice were cultured in the presence of 12.5, 25, or 50 μmol/L of oleuropein in a receptor activator of nuclear factor kappaB ligand (RANKL)induced osteoclast culture system, and the multinucleated cells were detected with tartrate resistant acid phosphatase (TRAP) staining. RAW264.7 cells were induced by RANKL in the presence of oleuropein at different concentrations, and the cell apoptosis was analyzed with flow cytometry. Osteoclastic resorption pits on the bone slices were observed with scanning electron microscopy, and the areas of osteoclastic bone resorption were determined using image processing software. The expressions of the osteoclastspecific protein cathepsin K(CTSK), nuclear factor of activated Tcells 1(NFATC1) and phosphorylated p38 were measured with Western blotting. ResultsRANKLinduced osteoclastogenesis was significantly inhibited by oleuropein in a dosedependent manner(P<005). The protein expression level of CTSK in RAW264.7 cells was downregulated by oleuropein(P<005), which produced no obvious effect on apoptosis of osteoclast cells(P>005). Oleuropein markedly decreased the area of bone resorption pits(P<005) and downregulated the protein expression of NFATC1 and phosphorylated p38 in the osteoclasts. ConclusionOleuropein inhibits RANKLinduced osteoclastogenesis and bone resorption in vitro possibly by downregulating the p38/MAPK signaling pathway.

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更新日期/Last Update: 2017-07-24