[1]金浩龙,陈志谨,熊昆,等.萋-尼染色导致的总挥发性有机物污染及其控制方法[J].第三军医大学学报,2017,39(14):1481-1485.
 JIN Haolong,CHEN Zhijin,XIONG Kun,et al.Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures[J].J Third Mil Med Univ,2017,39(14):1481-1485.
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萋-尼染色导致的总挥发性有机物污染及其控制方法(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第14期
页码:
1481-1485
栏目:
临床医学
出版日期:
2017-07-30

文章信息/Info

Title:
Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures
作者:
金浩龙陈志谨熊昆金晓琳
第三军医大学新桥医院感染控制科;400038 重庆,第三军医大学基础医学部微生物学教研室,重庆市微生物工程实验室
Author(s):
JIN Haolong CHEN Zhijin XIONG Kun JIN Xiaolin

Department of Infection Control, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037; Department of Microbiology, Chongqing  Laboratory of Microbiological Engineering, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China

关键词:
萋-尼染色TVOC污染冷染色法
Keywords:
Ziehl-Neelsen stainingtotal volatile organic compoundscold staining
分类号:
R136.32;R446
文献标志码:
A
摘要:

目的建立一种改良抗酸染色冷染方法,用以控制实验室总挥发性有机物(total volatile organic compound,TVOC)污染。方法采用TVOC检测仪定量测定实验室内空气中的苯系物和TVOC污染程度;以卡介苗(bacillecalmetteguerin,BCG)为染色标本,配制含有1% Triton X100标本稀释液和初染液,建立不用加温助染的抗酸染色冷染法替代传统抗酸染色法。结果传统萋尼抗酸染色时,室内TVOC污染在12 min时间点即高达1 014 ppb(约1 mg/m3),成倍超过国家室内卫生标准(GB503252010)规定值(500 ppb, 即0.5 mg/m3);同样条件下,用改良冷染法染色期间,室内TVOC量有所增加,但在试验期间最高值为387 ppb, 保持在国家室内卫生标准容许限度以下;利用Triton X100建立的改良抗酸冷染法具有抗酸染色特性,其对抗酸杆菌的可检测度为3×10-6~6×10-5 CFU/mL。结论抗酸染色冷染法可用于替代传统萋尼抗酸染色,从而控制实验室的TVOC污染问题。

Abstract:

ObjectiveTo detect total volatile organic compound (TVOC) pollution in the laboratory during ZiehlNeelsen acidfast staining and establish an improved method for controlling TVOC pollution. MethodsTVOC concentration in the room air of the laboratory was determined using RAE portabletype instrument during the performance of routine acidfast staining of Bacille CalmetteGuerin (BCG). A dilution solution and a carbolfuchsin solution containing 1% Triton X100 were prepared for use in the modified cold acidfast staining without heating, and the TVOC pollution level caused by the modified method and the routine acidfast staining method was compared. ResultsAt 12 min after the start of routine acidfast staining, the concentration of TVOC in the room air of the laboratory reached the highest level of 1 014 ppb (about 1 mg/m3), which was 2 folds of the permissible exposure limit (500 ppb, 0.5 mg/m3) in the National Standard (GB503252010). During the performance of the modified cold acidfast staining, TVOC  concentration in the laboratory increased only slightly and remained under the permissible exposure limit. The detection sensitivity of BCG ranged from   3×10-6  to 6×10-5 CFU using the modified cold staining method. ConclusionThe modified acidfast cold staining method can be used as an alternative to the conventional acidfast staining for controlling TVOC pollution in the laboratory.

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更新日期/Last Update: 2017-07-24