[1]汪君君,谢薇佳,袁帅,等.miRNA基因单核苷酸多态性与非小细胞肺癌易感性的关联研究[J].第三军医大学学报,2017,39(09):904-912.
 Wang Junjun,Xie Weijia,Yuan Shuai,et al.Genetic association of single nucleotide polymorphisms in miRNAs with NSCLC susceptibility[J].J Third Mil Med Univ,2017,39(09):904-912.
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miRNA基因单核苷酸多态性与非小细胞肺癌易感性的关联研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第09期
页码:
904-912
栏目:
临床医学
出版日期:
2017-05-15

文章信息/Info

Title:
Genetic association of single nucleotide polymorphisms in miRNAs with NSCLC susceptibility
作者:
汪君君谢薇佳袁帅刘庆云向颖吴龙邬娜李成英余祖滨白莉李亚斐
第三军医大学军事预防医学院军队流行病学教研室;第三军医大学新桥医院:胸外科,全军呼吸内科研究所,全军呼吸病研究重点实验室
Author(s):
Wang Junjun Xie Weijia Yuan Shuai Liu Qinyun Xiang Ying Wu Long Wu Na Li Chengying Yu Zubin Bai Li Li Yafei

Department of Epidemiology, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038; Department of Thoracic Surgery, Institute of Respiratory Diseases, Key Laboratory of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
非小细胞肺癌微小RNA单核苷酸多态性 遗传易感性
Keywords:
non-small cell lung cancer miRNA single nucleotide polymorphisms genetic predisposition to disease
分类号:
R181.33;R394.5;R734.2
文献标志码:
A
摘要:

目的       探讨微小RNA(microRNA,miRNA)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与非小细胞肺癌(non-small cell lung cancer,NSCLC)发生风险的关系。 方法     通过生物信息学方法筛选出13个位于miRNA基因序列上的潜在功能性的SNPs位点。采用病例-对照研究设计,纳入新发NSCLC患者626例,对照为736例同期门诊体检人群。提取研究对象外周血细胞基因组DNA,采用SNPscanTM对筛选的13个SNPs位点进行基因分型,多因素Logistic回归分析SNPs与肺癌发生风险的关联性和关联强度。 结果      发现3个SNPs,即rs62085660(位于miRNA AC145343.1)、rs11597888(位于miRNA AL391839.1)和rs16867808(位于miRNA AL021918.2)与NSCLC发生风险显著相关,但此种关联只限于特定的亚组人群。rs62085660(C/G)位点与肺鳞癌的发生风险相关(加性模型,校正OR=0.79,95%CI=0.62~1.00,P=0.049;显性模型,校正OR=0.72,95%CI=0.53~0.98,P=0.035)。rs11597888(G/A)和rs16867808(T/C)位点只在吸烟人群中与肺癌易感性相关(rs11597888:显性模型,校正OR=1.42,95%CI=1.03~1.96,P=0.033;rs16867808:加性模型,校正OR=0.58,95%CI=0.40~0.84,P=0.004,显性模型,校正OR=0.53,95%CI=0.35~0.81,P=0.004)。 结论        rs62085660位点与肺鳞癌发生风险相关,rs11597888位点和rs16867808位点与吸烟相关性肺癌发生风险相关。

Abstract:

Objective       To explore the association between single nucleotide polymorphisms (SNPs) in miRNA and the risk of non-small cell lung cancer (NSCLC). Methods      A total of 13 candidate SNPs of different miRNA genes were identified by bioinformatics analysis. A case-control study was performed in 626 patients with NSCLC and 736 controls. Genomic DNA was extracted from peripheral blood cells, and the SNPs were genotyped by SNPscanTM system. A multivariate logistic regression analysis was applied to evaluate the association between NSCLC risk and each selected SNP in miRNA level. Results      Of the 13 SNPs, 3 (rs62085660, rs11597888, and rs16867808) showed significant association with NSCLC risk, and this association was limited to specific subgroup of subjects. The SNP rs62085660 (C/G) showed a significant association with the risk of lung squamous carcinoma (additive model: ORadjusted=0.79, 95%CI=0.62~1.00, P=0.049; dominant model: ORadjusted=0.72, 95%CI=0.53~0.98, P=0.035). In smokers, rs11597888 (G/A) and rs16867808 (T/C) were significantly associated with NSCLC risk (rs11597888: dominant model, ORadjusted=1.42, 95%CI=1.03~1.96, P=0.033; rs16867808: additive model, ORadjusted=0.58, 95%CI=0.40~0.84, P=0.004; dominant model, ORadjusted=0.53, 95%CI=0.35~0.81, P=0.004). Conclusion      rs62085660 SNP may contribute to genetic susceptibility to lung squamous carcinomas, and rs16867808 SNP to genetic susceptibility to NSCLC in smoking population.

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更新日期/Last Update: 2017-05-08